Composite cellulase system and its application in the production of starch fuel ethanol

A technology of compound cellulase and compound cellulose, which is applied in the direction of biofuels, enzymes, enzymes, etc., can solve the problems of the influence of yeast growth and fermentation, high viscosity of sugar solution, and high solid content, and achieve the reduction of total sugar and viscosity, The effect of ethanol yield increase and efficient conversion

Active Publication Date: 2022-05-27
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the production process of corn fuel ethanol, corn cellulose has not been effectively utilized, and corn starch has not been fully degraded. In the later stage, problems such as high viscosity of sugar solution and high solid content appear, so the growth and fermentation of yeast are seriously affected.

Method used

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  • Composite cellulase system and its application in the production of starch fuel ethanol
  • Composite cellulase system and its application in the production of starch fuel ethanol
  • Composite cellulase system and its application in the production of starch fuel ethanol

Examples

Experimental program
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Effect test

Embodiment 1

[0042] The present invention provides a complex cellulase line prepared by the following method, the method comprising the following steps:

[0043] The aspergillus niger converted to feruliformin gene of juniperoidyces source and the dehydrogenase gene of juniperpenoidyces derived from penicillium is inoculated in fermentation medium II. fermentation culture was obtained by fermentation culture I., the fermentation culture liquid I. is a feruliformase containing a juniperpene source and a dehydrogenase transforming enzyme line of juniperceous penicillis, and a β - glucosidase of aspergillus niger source;

[0044] Trichoderma reticulate is inoculated in 3% of the inoculation amount in fermentation medium I. fermentation culture to obtain fermentation culture medium II., the fermentation culture liquid II. is a cellulase containing Trichoderma reedi source; and

[0045] The fermentation culture solution I. was compounded (well mixed) according to the volume ratio 1:10 to obtain a c...

Embodiment 2

[0052] The present invention provides a complex cellulase line prepared by the following method, the method comprising the following steps:

[0053] The aspergillus niger is inoculated with 4% of the inoculation amount in the fermentation medium II. fermentation culture was obtained by fermentation culture I., the fermentation culture liquid I. is a ferulita containing a juniperpene source and a dehydrogenase of a juniperpenis source of aspergillus niger, and a β - glucosidase of aspergillus niger;

[0054] Trichoderma reticulate according to the amount of inoculation 4% in the fermentation medium I. fermentation culture to obtain fermentation culture ii., the fermentation culture liquid II. is a cellulase containing Trichoderma reedi derived from; and

[0055] The fermentation culture solution I. was compounded with the fermentation culture medium II. according to the volume ratio of 1:8.5 (well mixed) to obtain a complex cellulase line.

[0056] Wherein, the fermentation medium ...

Embodiment 3

[0062] The present invention provides a complex cellulase line prepared by the following method, the method comprising the following steps:

[0063]The aspergillus niger is inoculated with 5% of the inoculation amount in the fermentation medium II. fermentation culture to obtain fermentation culture liquid I., the fermentation culture liquid I. is a ferulita containing a juniper penicillium source and a dehydrogenase of the juniperpenis source of aspergillus niger, and a β - glucosidase of aspergillus niger;

[0064] Trichoderma reticulate according to the amount of inoculation 5% in the fermentation medium I. fermentation culture to obtain fermentation culture ii., the fermentation culture liquid II. is a cellulase containing Trichoderma reedi derived from; and

[0065] The fermentation culture solution I. was compounded with the fermentation culture medium II. according to the volume ratio 1:7 (well mixed) to obtain a complex cellulase line.

[0066] Wherein, the fermentation me...

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Abstract

The invention discloses a composite cellulase system prepared by the following method. The method comprises the following steps: transforming the ferulic esterase gene derived from Penicillium juniperus and the dehydrogenase gene derived from Penicillium juniperus Aspergillus niger was fermented to obtain fermentation broth I; Trichoderma reesei was fermented to obtain fermentation broth II; and fermentation broth I and fermentation broth II were compounded according to a certain volume ratio to obtain a composite cellulase system. The composite cellulase system can effectively improve the Trichoderma reesei enzyme system, and then effectively increase the rate of degradation of crystalline cellulose by the Trichoderma reesei enzyme system. The present invention also provides the application of a compound cellulase system. In the process of synchronous saccharification and fermentation of yeast to produce starch fuel ethanol, the compound cellulase is added to reduce the viscosity of corn mash on the one hand and remove the remaining cellulose And further hydrolysis of starch to realize the efficient conversion from corn to fuel ethanol, thus bringing significant economic benefits to the ethanol fermentation industry.

Description

Technical field [0001] The present invention relates to the field of ethanol fermentation production technology, in particular to a complex cellulase system and its application in the production of starch fuel ethanol. Background [0002] Ethanol is an important bulk chemical product with a wide range of uses, commonly used as fuel, solvent and disinfectant, and also used to produce other compounds. Ethanol is also currently the world's largest fermentation product, China's emerging industry development plan points out that by 2020, the annual utilization of bio-liquid fuels will reach 12 million tons. Although the production process of starch ethanol fermentation is relatively mature, there are still problems in its production process. Corn cellulose in the corn fuel ethanol production process is not effectively utilized, corn starch is not fully degraded, and problems such as large viscosity of sugar liquid and many solids appear in the later stage, so the growth and fermentati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/63C12P7/10C12N9/42
CPCC12N9/2437C12P7/10Y02E50/10
Inventor 高乐张东远陈树林
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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