Green production process of glutamic acid
A green production, glutamic acid technology, applied in the green production process of glutamic acid, can solve the problems of low nutritional value of hydrolyzate, difficult to control the fermentation process, fermentation instability and other problems, to reduce high pigment content and less impurities , The effect of reducing industrial costs
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Embodiment 1
[0032] An amino acid fermentation medium, which includes a fermentation medium A and a fermentation medium B, the fermentation medium A and the fermentation medium B are used separately, and the amino acid is glutamic acid;
[0033] The fermentation medium B is to add inositol and glycerol on the basis of the fermentation medium A;
[0034] The fermentation medium A is: glucose 50g / L, Na 2 HPO 4 12H 2 O 2g / L, KCl 1g / L, MgSO 4 ·7H 2 O1g / L, MnSO 4 ·H 2 O 3mg / L, FeSO 4 ·7H 2 O 3mg / L, V B1 10mg / L, 1mg / L fulvic acid, 7μg / L biotin, 60ml / L enzymatic hydrolyzate, sterilized at 115°C for 15min;
[0035] The fermentation medium B is: glucose 50g / L, Na 2 HPO 4 12H 2 O 2g / L, KCl 1g / L, MgSO 4 ·7H2 O1g / L, MnSO 4 ·H 2 O 3mg / L, FeSO 4 ·7H 2 O 3mg / L, V B1 10mg / L, 1mg / L fulvic acid, 7μg / L biotin, 60ml / L cell enzymolysis solution, 100mg / L inositol, 500mg / L glycerin, sterilized at 115°C for 15min;
[0036] The preparation method of the thallus enzymatic solution is as follows: ...
Embodiment 2
[0039] The green production technique of glutamic acid, it comprises the steps:
[0040] 1) Brevibacterium flavum GDK-9 (sourced from Tianjin University of Science and Technology, the source of the strain can also refer to "Study on the variable temperature control process of L-glutamic acid fermentation, Tianjin Chemical Industry, 2010") was inoculated with 12% seed solution (OD 600nm 10) Connect to a 50L automatic fermenter equipped with 30L fermentation medium A for fermentation and cultivation, the fermentation temperature is 38°C, the ventilation ratio is 1:0.7, the stirring speed is 500r / min, and the dissolved oxygen is maintained at 20%. Coupled with the ceramic membrane, when the fermentation reaches 24 hours, the fermentation liquid A in the fermenter is separated through the ceramic membrane to obtain the filtrate A and the concentrated bacteria, the filtrate A is discharged, the concentrated bacteria are returned to the fermenter, and at the same time, the fermenter ...
Embodiment 3
[0044] Influence of hydrolysis process on bacterial proteolysis components:
[0045] Set up a control group,
[0046] Matched group 1: do not adopt high-speed shearing machine to process, all the other are the same as embodiment 1;
[0047] Control group 2: Hydrochloric acid with a concentration of 5 mol / L was used for hydrolysis for 6 hours.
[0048] The wall breaking rate, protein content and total free amino acid content are shown in Table 1:
[0049] group Example 1 Control group 1 Control group 2 Broken rate% 97.2 83.4 68.7 Protein content mg / g (dried bacteria) 226.1 285.9 268.5 Total free amino acid content mg / g (dried bacteria) 371.5 256.3 187.4
[0050] Visible by table 1, utilize high-speed shearing machine to shear and process 120s, can greatly improve cell wall breaking rate, thereby improve hydrolysis efficiency; The present invention adopts dilute hydrochloric acid to carry out pretreatment, and used acid concentration ...
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