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Application of SMXP-58 bacterial strain in aspect of degrading cellulose in tobacco leaves

A SMXP-58, cellulose technology, applied in the application, tobacco, bacteria and other directions, can solve the problem of irreversible influence on the quality of cigarette products, and achieve the effect of promoting the quality improvement of cigarette raw materials, shortening the processing period, and degrading the cellulose content well. Effect

Active Publication Date: 2019-03-29
CHINA TOBACCO HENAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in actual use, due to the special quality requirements of cigarette products, the quality of final cigarette products will often be irreversibly affected after cellulase is used to degrade cellulose

Method used

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  • Application of SMXP-58 bacterial strain in aspect of degrading cellulose in tobacco leaves
  • Application of SMXP-58 bacterial strain in aspect of degrading cellulose in tobacco leaves
  • Application of SMXP-58 bacterial strain in aspect of degrading cellulose in tobacco leaves

Examples

Experimental program
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Effect test

Embodiment 1

[0022] In order to use the SMXP-58 strain to degrade cellulose in tobacco leaves, it is first necessary to prepare the strain into an easy-to-use bacterial agent, and the specific preparation process is as follows.

[0023] (1) Activated strains: put the SMXP-58 strain on the LB plate, and activate it in a biochemical climate chamber at 37°C for 1 day;

[0024] (2) Preparation of seed liquid: Scrape the bacteria cultured in step (1) in the ultra-clean workbench, inoculate in a 18×180 test tube containing 5 mL of liquid LB medium, and place on a shaker at 30°C and 150 rpm Overnight culture;

[0025] (3) Expansion culture: Transfer the seed solution in step (2) to a sterile 250mL Erlenmeyer flask containing 100mL liquid LB medium at the ratio of 1 mL bacterial suspension / 100 ml LB medium (1% volume ratio) medium, 30°C, 150 rpm, cultured to OD 600 = about 2.0;

[0026] (4) Preparation of bacterial agent: Centrifuge the bacterial solution in step (3) at 3600 rpm for 10 minutes,...

Embodiment 2

[0028] When utilizing the bacterial agent prepared in the embodiment 1 to process tobacco leaves, the concrete steps are:

[0029] Take the tobacco leaves after extracting the tobacco stems, divide them into sheets, and then evenly divide them into multiple portions, each portion is 100 g;

[0030] According to the tobacco leaf mass ratio of 2%, the microbial inoculant agent prepared in Example 1 is evenly sprayed on the surface of the treated tobacco leaf with a sprayer, and then the moisture content of the tobacco leaf is adjusted to 25%; Treat for 20 minutes to inactivate the bacteria and the cellulase produced by them.

[0031] In order to determine the temperature and humidity conditions and time of the optimal effect of the bacterial agent, the inventors took the fermentation time, fermentation temperature, and fermentation humidity as influencing factors respectively, and carried out a three-factor level design. The specific design is as follows:

[0032] Table 1 Facto...

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Abstract

The invention belongs to the technical field of cigarette raw material production and processing, and particularly relates to application of the specific bacillus subtilis SMXP-58 bacterial strain inthe aspect of degrading cellulose in tobacco leaves. During application, the bacillus subtilis SMXP-58 bacterial strains are prepared into a microbial agent containing living bacteria, the microbial agent is mixed with the tobacco leaves, then, fermentation processing is performed for degrading cellulose in tobacco leaves. The invention belongs to development of the novel application of existing known strains. The bacterial strain can well lower the content of pectin in tobacco stems, the content of cellulose in tobacco leaves can be well lowered within the short period of time, the cellulosedegrading rate can reach about 30%, and the technical effect of well lowering the content of cellulose in tobacco leaves is achieved. Detection results further show that the cellulose is finally degraded into saccharide ingredients, after degradation processing, the total sugar in tobacco leaves and the content of reducing sugar are obviously improved, the sensory quality of tobacco leaves is stable or even improved, and the stability of the final cigarette product quality is ensured.

Description

technical field [0001] The invention belongs to the technical field of cigarette raw material production and processing, and specifically relates to a patent application for the application of a specific strain of Bacillus subtilis SMXP-58 in degrading cellulose in tobacco leaves. Background technique [0002] Cellulose is the main structural component of tobacco leaves, together with hemicellulose, lignin and other substances, it forms the tobacco cell wall, determines the integrity of tobacco leaves, and its content is closely related to the quality of tobacco. [0003] Studies have shown that cellulose has a positive effect on the combustibility of tobacco leaves, which can increase the firepower of tobacco leaves; but when the cellulose content is too high, it has more negative effects on the quality of tobacco leaves, mainly as follows: too high a cellulose content can easily cause Tobacco leaf tissue is broken, which affects the processing plasticity; on the other hand...

Claims

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Application Information

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IPC IPC(8): A24B15/20C12N1/20C12R1/125
CPCA24B15/20C12N1/20
Inventor 杨宗灿张婷婷冯颖杰张展李家美申洪涛彭玉富张耀广
Owner CHINA TOBACCO HENAN IND
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