Unlock instant, AI-driven research and patent intelligence for your innovation.

Microbial composite preparation immobilized by graphene oxide nanocomposite material, and preparing method and application thereof in coking wastewater

A nano-composite material and composite preparation technology, applied in the field of microorganisms, can solve the problems of low recovery rate, high COD value of coking wastewater, poor degradation efficiency, etc., and achieve the effect of high removal rate, good removal effect and good adsorption.

Active Publication Date: 2019-03-29
SHANXI UNIV
View PDF12 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the deficiencies of the existing technology, solve the technical problem of high COD value of coking wastewater, and overcome the defects of poor degradation efficiency and low recovery rate in the prior art, the present invention provides a microbial composite preparation immobilized by graphene oxide nanocomposites , preparation method and its application in coking wastewater

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A kind of biocomposite preparation that graphene oxide nanocomposite material is fixed, is prepared according to the following steps:

[0029] S1. Preparation of liquid fermentation medium: Weigh raw materials: 3.0g beef extract, 10.0g peptone, 5.0g sodium chloride, 0.7g potassium chloride, 0.03g MnSO 4 ·H 2 0, 1.0gNH 4 NO 3 , Dissolve the weighed raw materials in 1000mL distilled water, adjust the pH value of the mixed solution to 7.2, and press 205.8kPa pressure (ie 2.1kg / cm 2 ) for 30 minutes of sterilization;

[0030] Preparation of solid slant and plate medium: Weigh raw materials: 3.0g~5.0g beef extract, 10.0g peptone, 5.0g~10.0g sodium chloride, 17.0g~18.0g agar, dissolve the weighed raw materials in 1000mL distilled water , adjust the pH value of the mixed solution to 7.0~7.2, and sterilize at 205.8kPa pressure for 30min in an environment of 121°C;

[0031] S2. Preparation of wet bacteria: First, take out the Citrobacter and Pseudomonas strains stored at -2...

Embodiment 2

[0043] A kind of biocomposite preparation that graphene oxide nanocomposite material is fixed, is prepared according to the following steps:

[0044] S1. Preparation of liquid fermentation medium: Weigh raw materials: 3.0g beef extract, 10.0g peptone, 5.0g sodium chloride, 0.7g potassium chloride, 0.03g MnSO 4 ·H 2 0, 1.0gNH 4 NO 3 , Dissolve the weighed raw materials in 1000mL distilled water, adjust the pH value of the mixed solution to 7.2, and sterilize at 205.8kPa pressure for 30min in an environment of 121°C;

[0045] Preparation of solid slant and plate medium: Weigh raw materials: 3.0g~5.0g beef extract, 10.0g peptone, 5.0g~10.0g sodium chloride, 17.0g~18.0g agar, dissolve the weighed raw materials in 1000mL distilled water , adjust the pH value of the mixed solution to 7.0~7.2, and sterilize at 205.8kPa pressure for 30min in an environment of 121°C;

[0046] S2. Preparation of wet bacteria: First, take out the Citrobacter and Pseudomonas strains stored at -20°C re...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Removal rateaaaaaaaaaa
Removal rateaaaaaaaaaa
Login to View More

Abstract

The invention relates to a microbial composite preparation immobilized by a graphene oxide nanocomposite material, and a preparing method and an application thereof in coking wastewater, belongs to the microbial technical field, and overcomes the defects of high COD value, poor degradation efficiency and low recovery rate of coking wastewater in the prior art. The preparation method comprises thesteps: firstly, a graphene oxide nanocomposite material is prepared; and secondly, the immobilized biological composite preparation is prepared. The graphene oxide nanocomposite material is used for immobilizing citrobacter and pseudomonas to obtain the microbial composite preparation. The immobilized microorganism has higher COD removal rate and better removal effect for coking wastewater effluent. The immobilized citrobacter and pseudomonas can be recycled for at least five times, and good adsorption and desorption effects are maintained. According to the microbial composite preparation immobilized by the prepared graphene oxide nanocomposite material, the effluent of a secondary sedimentation tank of a coking wastewater treatment plant is studied, and the COD removal rate after the reaction is 68%-78%.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a microbial composite preparation immobilized by a graphene oxide nanocomposite material, a preparation method and its application in coking wastewater. Background technique [0002] As we all know, coking wastewater is composed of complex organic pollutants, which are stable in nature, some of which are toxic and carcinogenic, and usually have a high COD value. Therefore, coking wastewater is a typical difficult and toxic wastewater. Immobilized microbial technology treats difficult-to-remove toxic and harmful organic pollutant wastewater, which has the advantages of high treatment efficiency and no secondary pollution, so it has good application and research prospects. [0003] The treatment of coking wastewater has been widely concerned by experts in the field of sewage treatment at home and abroad, but no breakthrough has been made after decades of research...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12N11/14C02F3/34C12R1/38C12R1/01
CPCC12N1/20C12N11/14C02F3/34
Inventor 李勇庞聪乐梁文婷董川
Owner SHANXI UNIV