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A kind of salt-tolerant and growth-promoting compound bacterial agent and its preparation method and application

A composite bacterial agent and composite bacterial technology, applied in biochemical equipment and methods, methods based on microorganisms, applications, etc., can solve the problems of poor effect and single function, and achieve cost reduction, fresh weight increase, and large specific surface area Effect

Active Publication Date: 2020-07-17
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to overcome the defects of single action and poor effect of the existing microbial bacterial agents, the present invention provides a salt-tolerant growth-promoting compound bacterial agent, which can effectively improve soil salinity through the synergistic effect of biochar and salt-tolerant growth-promoting compound bacteria. Alkalinization can also significantly improve crop quality and nutrition in saline-alkali soil

Method used

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  • A kind of salt-tolerant and growth-promoting compound bacterial agent and its preparation method and application
  • A kind of salt-tolerant and growth-promoting compound bacterial agent and its preparation method and application
  • A kind of salt-tolerant and growth-promoting compound bacterial agent and its preparation method and application

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preparation example Construction

[0045] Specifically, the present invention provides a method for preparing biochar capable of obtaining the characteristics of large specific surface area, large pore diameter and large pore volume, comprising the following steps:

[0046] The agricultural waste is pyrolyzed at a constant temperature of 300-600°C under the condition of anaerobic, and crushed to obtain biochar.

[0047] In the present invention, the agricultural waste includes but not limited to one or more of straw, jujube wood and grape branches. The invention adopts discarded agricultural wastes as raw materials for biochar preparation, which is more environmentally friendly and realizes resource recycling. In the present invention, the agricultural waste is preferably crushed before constant temperature pyrolysis; preferably, the agricultural waste is crushed into debris with a maximum diameter of 2-3 cm, so as to fully react.

[0048] The invention directly carbonizes the agricultural waste through the co...

Embodiment 1

[0064] (1) Use beef extract (5g / L), peptone (10g / L), sodium chloride (5g / L) liquid medium to cultivate three kinds of single bacterial liquid, SRPG-396 bacterial liquid 20mL, SRPG-70 bacterial liquid 10mL , SRPG-4 bacterial solution 20mL, culture temperature 30℃, pH value 7.0, ferment for 24h, the concentration of viable bacteria of a single salt-tolerant strain is 1×10 9 CFU / mL. Mix the above-mentioned fermented bacterial liquids to obtain a salt-tolerant and growth-promoting compound bacterial liquid. 9 CFU / mL.

[0065] Cut 250g of cotton stalks into pieces of 2-3cm, put them into a sealed iron can, and put them into N for 10 minutes 2 to exhaust O 2 , and then reach the set temperature of 450° C., constant temperature pyrolysis for 2 hours, after cooling down, pulverize with a pulverizer, and the particle size of the biochar is 100 mesh. The specific surface area of ​​the obtained cotton straw biochar is 1.5101 m 2 / g, the average pore diameter is Single point pore v...

Embodiment 2

[0070] (1) Use beef extract (5g / L), peptone (10g / L), sodium chloride (5g / L) liquid medium to cultivate three kinds of single bacterial liquid, SRPG-396 bacterial liquid 30mL, SRPG-70 bacterial liquid 20mL , SRPG-4 bacterial solution 40mL, culture temperature 30 ℃, pH value 7.0, fermented for 24h, the concentration of viable bacteria of a single salt-tolerant strain was 1×10 9 CFU / mL. Mix the above fermented bacterial liquids to obtain a salt-tolerant and growth-promoting compound bacterial liquid. 9 CFU / mL.

[0071] Cut jujube 250g into 2-3cm, put it into a sealed iron can, and put it into N for 10 minutes 2 to exhaust O 2 , and then reach the set temperature of 400° C., constant temperature pyrolysis for 2 hours, after cooling down, pulverize with a pulverizer, and the particle size of the biochar is 100 mesh. The specific surface area of ​​the obtained jujube wood biochar is 1.4232m2 / g, and the average pore diameter is Single point pore volume is 0.006985cm 3 / g.

[...

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Abstract

The present invention provides a salt-tolerant growth-promoting composite bacterium agent, a preparation method and an application thereof, and relates to the technical field of composite bacterium agents. The salt-tolerant growth-promoting composite bacterium agent comprises biochar and salt-tolerant growth-promoting composite bacteria loaded in the biochar; a total number of viable bacteria of the salt-tolerant growth-promoting composite bacterium agent is larger than or equal to 8x10<8> CFU / g; and the salt-tolerant growth-promoting composite bacteria comprise pseudomonas putida SRPG-396, enterobacter cloacae SRPG-70 and raoultella planticola SRPG-4. The biochar can be used as a salt-tolerant growth-promoting composite bacterium carrier to improve survival rate of the salt-tolerant growth-promoting composite bacteria in saline-alkali soil, besides, the biochar and salt-tolerant growth-promoting composite bacteria all have functions of improving the saline-alkaline soil and promotingplant growth, have synergistic interaction after compounding, can significantly improve an improving effect of the saline-alkali soil and quality and nutrients of crops growing in the saline-alkali soil, and have good environmental and social benefits. The prepared salt-tolerant growth-promoting composite bacterium agent has a long storage time.

Description

technical field [0001] The invention relates to the technical field of composite bacterial agents, in particular to a salt-tolerant growth-promoting composite bacterial agent and its preparation method and application. Background technique [0002] Continuous cropping, excessive fertilization, and improper irrigation in agricultural production cause problems such as soil salinization, quality degradation, and micro-ecological damage, especially in arid and semi-arid areas. Yield decline, and excessive application of chemical fertilizers also causes melons to be unsweet and fruits are not fragrant, which seriously restricts modern agricultural production and sustainable economic development. [0003] In recent years, studies have shown that microbial agents can significantly promote the growth of crops and alleviate the harm of salt stress through direct or indirect effects. However, free living microbial cells are the most sensitive to environmental changes, and are extreme...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N11/14C05G3/80C05G3/00C12R1/40C12R1/01
CPCC05D9/00C05G3/00C05G3/80C12N1/20C12N11/14C05F11/08
Inventor 武占省魏萌萌陶思远王文飞
Owner SHIHEZI UNIVERSITY
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