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A kind of method for preparing lycopene

A technology of lycopene and seeds, which is applied in the field of preparing lycopene by using B. trispora, can solve problems such as difficulty in industrial production, damage to bacteria, increased cost, etc., to improve the distribution of mycelia and improve the quality of mycelia. The degree of dispersion and the effect of improving joining efficiency

Active Publication Date: 2021-09-21
CABIO BIOTECH WUHAN CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The usual measure to increase dissolved oxygen is to increase blowing or forced oxygen ventilation, but this method consumes a lot of energy, increases costs, and makes it difficult for industrial production
There are also oxygen carriers n-dodecane added to the fermentation broth, but it will cause damage to the bacteria
There are also many reports of adding precursor substances, but the effect is not very satisfactory, and it will increase the cost

Method used

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  • A kind of method for preparing lycopene
  • A kind of method for preparing lycopene

Examples

Experimental program
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Effect test

Embodiment 1

[0026] 1) Slant culture: prepare PDA slant medium (glucose 20g / L, agar powder 25g / L, peeled potatoes 200g / L; cut potatoes into 1cm cubes, add deionized water and boil for 30 minutes, filter with four layers of gauze after cooling , take the filtered supernatant and add glucose and agar powder). Take the spore suspensions of B. trispora positive bacteria and B. trispora negative bacteria, spread them on the PDA slant medium respectively, and cultivate them in a constant temperature incubator at 25°C for 5-7 days;

[0027] 2) Seed culture: Use an inoculation spatula to scoop up a shovel of positive bacteria and negative bacteria from the slant of the positive and negative strains of B. trispora respectively, and inoculate them into 1000ml Erlenmeyer flasks containing 150ml of seed medium respectively, at 25°C , and cultivated for 48 hours under the condition of 180 revs / min, to obtain the positive bacteria seed liquid of the three-spora B. trispora and the negative bacteria seed...

Embodiment 2

[0036] 1) Slant culture: prepare PDA slant medium (glucose 20g / L, agar powder 25g / L, peeled potatoes 200g / L; cut potatoes into 1cm cubes, add deionized water and boil for 30 minutes, filter with four layers of gauze after cooling , take the filtered supernatant and add glucose and agar powder). Take the spore suspensions of B. trispora positive bacteria and B. trispora negative bacteria, spread them on the PDA slant medium respectively, and cultivate them in a constant temperature incubator at 25°C for 5-7 days;

[0037] 2) Seed culture: Shovel a shovel of positive bacteria and negative bacteria from the slant surface of the positive bacteria and negative bacteria strains of B. trispora respectively, and inoculate them into 1000ml triangular flasks containing 150ml seed culture medium respectively. Cultivate for 48 hours under the condition of 25°C and 180 rev / min, to obtain positive bacteria seed liquid of B. trispora and negative bacteria seed liquid of B. trispora. The see...

Embodiment approach 1

[0044] Scheme 1 of this embodiment: Grinding the positive and negative seed liquids of B. trispora with a colloid mill respectively, with a grinding tooth gap of 0.01 mm, 2000 rpm, and 4 times of circular grinding, and grinding the B. trispora after grinding The positive and negative seed liquids are mixed again for fermentation, and the yield of lycopene in the fermented liquid is measured to be 7.21g / L, which is 51.15% higher than that of the prior art.

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Abstract

The present invention relates to a method for preparing lycopene. The positive bacteria of B. trispora and the negative bacteria of B. trispora at the seed stage are treated by physical means respectively, and then mixed in a certain proportion for fermentation culture; the bacteria obtained after fermentation are collected and extracted from the bacteria. Lycopene. In the improved process of the invention, before mixing and fermenting the positive and negative bacteria of B. trispora, it is treated by physical means, and by reducing the entanglement between the mycelia in the bacteria body, the degree of dispersion of the mycelium is improved, and the degree of distribution of the mycelium is improved, In order to achieve the purpose of improving the joining efficiency and improving the fermentation yield.

Description

technical field [0001] The invention relates to a method for preparing lycopene, in particular to a method for preparing lycopene by using B. trispora. Background technique [0002] Lycopene is a fat-soluble carotenoid that can efficiently quench singlet oxygen and scavenge free radicals. Its antioxidant activity is the strongest among carotenoids. It has a certain effect on delaying aging and so on, and it is a functional natural pigment with great development value. [0003] The production of lycopene raw materials mainly includes plant extraction, chemical synthesis and microbial fermentation. The main raw material required by the plant extraction method is tomato. Tomato planting is limited by climate, place of origin, transportation and other conditions, and the extraction process is cumbersome and lengthy, and the cost is expensive, which cannot meet the needs of industrial production. Although the cost of chemical synthesis is low, its impact on the environment is r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P5/02C12N1/14C12N1/00C12R1/645
CPCC12N1/005C12N1/14C12P5/007
Inventor 李翔宇余超陆姝欢杨艳红汪志明
Owner CABIO BIOTECH WUHAN CO LTD
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