Nano-bioprobe for detecting pathogenic bacteria and preparation method thereof

A nano-biological probe, pathogenic bacteria technology, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve problems such as false positives and complex equipment, and achieve high sensitivity, environmental friendliness, and good application prospects. Effect

Active Publication Date: 2019-03-29
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above method also has problems such as complex equipment, false positives, etc., and requires operations such as pre-enrichment of bacteria, which cannot fully meet the needs of the market.

Method used

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  • Nano-bioprobe for detecting pathogenic bacteria and preparation method thereof
  • Nano-bioprobe for detecting pathogenic bacteria and preparation method thereof
  • Nano-bioprobe for detecting pathogenic bacteria and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] This embodiment provides a nanobiological probe for detecting Staphylococcus aureus, which consists of a magnetic core-shell nanostructure coupled with a specific antibody against Staphylococcus aureus, wherein the magnetic core-shell nanostructure is made of ferric oxide particles The core and the silicon dioxide layer embedded with platinum particles are the shell layer, which is negatively charged as a whole and has the activity of simulating peroxidase. Its preparation method comprises the following steps:

[0041] (1) Disperse iron ferric oxide nanoparticles (1mg / mL, 10mL) dispersed in cyclohexane in 10mL polyethylene glycol octylphenyl ether (Triton X-100) / n-hexanol / water by mechanical stirring In the mixed solution (Triton X-100, n-hexanol, water molar ratio is 1:2:6) to form a reverse microemulsion system, then add 200 μL of ammonia water (28% by mass fraction) and 200 μL of ethyl orthosilicate to make positive Ethyl silicate is hydrolyzed in an alkaline enviro...

Embodiment 2

[0049] This embodiment provides a nano-biological probe for detecting Staphylococcus aureus, the difference from Example 1 is that in step (3), it is positively charged ferric oxide-silicon dioxide particles (1mg / mL, 2mL ) and negatively charged platinum nanoparticles (1 mg / mL, 2 mL) were mixed for 14 h.

[0050] image 3 For the Fe prepared in this embodiment 3 o 4 TEM image of @Si-Pt. As can be seen from the figure, the Fe prepared in this example 3 o 4 The particle size of @Si-Pt is relatively uniform, and there are more platinum particles on the surface, but some of them are aggregated, which makes the overall particle shape no longer uniform, which is not conducive to the later application in biological systems.

Embodiment 3

[0052] This embodiment provides a nano-biological probe for detecting Staphylococcus aureus, the difference from Example 1 is: polyethylene glycol octylphenyl ether (Triton X-100) / n-hexanol / water in step (1) The molar ratio of Triton X-100, n-hexanol and water in the mixed solution is 1:2:3.

[0053] Figure 4 For the Fe prepared in this embodiment 3 o 4 TEM image of @Si-Pt. It can be seen from the figure that the reverse microemulsion system formed in this example is not ideal, resulting in poor coating effect of silica and formation of multiple cores.

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Abstract

The invention provides a nano-bioprobe for detecting pathogenic bacteria and a preparation method thereof. The nano-bioprobe is composed, in a coupling manner, a magnetic core-shell nanostructure anda specific recognition molecule targeted to the pathogenic bacteria; the magnetic core-shell nanostructure is prepared with a Fe3O4 particle being a core and a SiO2 layer, in which platinum nanoparticles are embedded, being a shell layer, the whole nanostructure carries negative electricity and has mimic peroxidase activity. The nano-bioprobe has the mimic peroxidase activity, so that existence ofthe pathogenic bacteria is quickly detected through a colorimetric method. The preparation method is simple in process, has good operability and is environment-friendly, wherein the nano-bioprobe canquickly detect the pathogenic bacteria and has excellent application prospect.

Description

technical field [0001] The invention relates to the field of preparation of novel nanomaterials, in particular to a nanobiological probe for detecting pathogenic bacteria and a preparation method thereof. Background technique [0002] Pathogens are microorganisms that can cause diseases, also known as pathogenic microorganisms, including bacteria, viruses, spirochetes, rickettsia, chlamydia, mycoplasma, fungi, and actinomycetes. [0003] Pathogenic bacteria not only seriously damage human health, but also have a major impact on the economy, and have become one of the most prominent public health problems in the world. The traditional method to detect pathogenic bacteria (such as Escherichia coli, Staphylococcus aureus, etc.) first needs to collect samples for enrichment culture, so that the number of detected bacteria can reach a detectable level in the sample, and then can be observed according to the morphological characteristics and a series of The identification of phys...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18G01N33/569G01N33/535C12R1/445C12R1/19
CPCG01N33/535G01N33/569G01N33/56911G01N33/56938C12Q1/18G01N2333/31G01N2333/245Y02A50/30
Inventor 杨蓉韩秋森王新环刘学良王琛
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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