Method for fermenting production of feed enzyme preparation by immobilized cell technology

A technology for immobilizing cells and feed enzyme preparations, applied in microorganism-based methods, biochemical equipment and methods, immobilization on/in organic carriers, etc., can solve the problems of low production efficiency, high production cost, and production technology. complex problems, to achieve the effect of improving production efficiency, prolonging service life, and easy mass transfer
CN109554302AActive Publication Date: 2019-04-02佳纬生物技术有限公司 +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
佳纬生物技术有限公司
Publication Date
2019-04-02

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Abstract

The invention discloses a method for fermenting production of a feed enzyme preparation by an immobilized cell technology, wherein the method comprises the following steps: S1, activation and enlargedculture of strains; S2, preparation of immobilized cells; S3, electrification culture of the immobilized cells; S4, fermentation enzyme-production culture; S5, separation and purification of an enzyme preparation; and S6, recycling. The immobilized cell technology is used for immobilizing aspergillus niger, trichoderma koningii, bacillus subtilis and bacillus natto together in carrier granules, and mixed fermentation is carried out to produce the feed enzyme preparation; with mutually beneficial symbiosis among the strains, the variety of produced enzymes is abundant. Compared with a method of single fermentation to produce various enzymes and then compounding, the method can simplify the production process, shorten the production cycle and greatly improve the production efficiency. The prepared immobilized cell particles have the advantages of good stability and easy separation and recovery, can be recycled for enzyme production, greatly reduce production costs, and have good application prospects in the field of feed enzyme preparation production.
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Description

technical field

[0001] The invention belongs to the field of feed additive production, and in particular relates to a method for fermenting and producing feed enzyme preparation using immobilized cell technology. Background technique

[0002] Feed enzyme preparation is a feed additive with enzyme as the main functional factor. The source of enzyme includes plants, animals and microorganisms, etc., which can be obtained by extraction or fermentation. Studies have shown that feed enzyme preparations are non-toxic, harmless, and non-residual to animals, and will not have negative effects on animals. First of all, the essence of the enzyme preparation is protein, which will be decomposed into amino acids and polypeptides after entering the animal's digestive tract and will not remain after being absorbed by the body; secondly, the enzyme preparation added to the feed belongs to exogenous enzymes. There are some differences from the endogenous enzymes secreted by animals themsel...

Claims

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