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Preparation method of biological nano-ferrous sulfide

A technology of ferrous sulfide and bio-nano, applied in the biological field, can solve the problems of biological activity, difficult to effectively control the synthesis rate, poor catalytic performance of nano-materials, etc., and achieve the effect of controllability

Inactive Publication Date: 2019-04-02
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the main problem of these biosynthesized nano-ferrous sulfides is that the catalytic performance of the synthesized nano-materials is poor, and both ferric iron and thiosulfate exist in the form of ions during the synthesis process, and the synthesis of nano-ferrous sulfide is only limited by Influenced by biological metabolism and biological activity, it is difficult to effectively control the synthesis rate

Method used

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  • Preparation method of biological nano-ferrous sulfide

Examples

Experimental program
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Effect test

Embodiment 1

[0029] (1) Strain used: Shewanella oneidensis MR-1, purchased from ATCC American Type Culture Collection, strain number ATCC700550;

[0030] (2) Cultivation of Shewanella: Add Shewanella to 200mL of LB medium (containing yeast extract 5g / L, tryptone 10g / L and sodium chloride 5g / L, pH=7.0) cultured at a temperature of 30°C and a shaking speed of 200 rpm for 12 hours to obtain a bacterial liquid;

[0031] (3) Construction of phosphate-ferric iron slow release system: Na 2 HPO 4 12H 2 O 17.8g / L, KH 2 PO 4 3g / L, NaCl 0.5g / L, NH 4 Cl 0.5g / L, 10mM sodium lactate, 0.1mM CaCl 2 , 1mM MgSO 4 , 1mM ferric chloride;

[0032] (4) Control of hydrogen sulfide release rate: add sodium thiosulfate to the slow release system of phosphate-ferric iron, and control the final concentration of sodium thiosulfate at 0.5mM;

[0033] (5) Control of the concentration of Shewanella: Take out the cultivated Shewanella, and according to the concentration conversion, add the bacteria slime to the...

Embodiment 2

[0041] It is basically the same as Example 1, but with the following changes: add sodium thiosulfate to the slow release system of phosphate-ferric iron at a final concentration of 1 mM, and synthesize the second type of nano-ferrous sulfide, which is designated as Type II.

Embodiment 3

[0043] It is basically the same as Example 1, but with the following changes: adding sodium thiosulfate to the slow release system of phosphate-ferric iron with a final concentration of 5mM to synthesize the third type of nano ferrous sulfide, which is designated as type three.

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Abstract

The invention belongs to the field of biological nano-materials, and specifically relates to a preparation method of biological nano-ferrous sulfide. The preparation method comprises the specific steps: firstly, a phosphate-ferric sustained release system is constructed; secondly, shewanella oneidensis MR-1 is inoculated into an LB culture medium, subjected to activation culture, and then inoculated into the phosphate-ferric sustained release system under aseptic and anaerobic conditions, and when the OD600 value of the final concentration of the shewanella oneidensis MR-1 in the system is 0.1-2, inoculation with the shewanella oneidensis MR-1 stops; and then sodium thiosulfate mother liquor is added, and the biological nano ferrous sulfide is obtained by culturing, centrifuging and washing. The novel phosphate-ferric sustained release system is constructed to realize the quantitative control of ferric iron. A series of nano-ferrous sulfide with different catalytic properties can be controllably bio-synthesized by adjusting the adding amount of the sulfur source in synthesis, the catalytic performance is effectively regulated, and a new way is opened up for regulation of the catalytic performance of the bio-synthesized nano-material.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of bionano ferrous sulfide. Background technique [0002] In recent years, with the acceleration of industrialization and urbanization, there are more and more toxic and harmful substances in groundwater and soil, which has become the primary problem of the environment and public health. Nanoscale ferrous sulfide has attracted attention from the environmental field due to its unique properties, such as small particle size, large specific surface area, efficient surface reduction reaction, and underground soil transport capacity. At present, nano-ferrous sulfide has been widely used in the treatment of heavy metals, chlorinated organic compounds (COCs), arsenic, selenium and other pollutants in groundwater and soil. The traditional chemical synthesis methods of nano-ferrous sulfide mainly include high-temperature direct synthesis and liquid phase synt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12R1/01
CPCC12P3/00
Inventor 程千文雍阳春俞洋洋
Owner JIANGSU UNIV
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