Preparation method of probiotics additive applied for livestock breeding

A technology of probiotics and additives, which is applied in the field of preparation of probiotics additives, can solve the problems of payment and processing costs, cumbersome processing procedures, etc., and achieve the effects of reducing feed-to-meat ratio, improving breeding efficiency, and increasing quantity

Pending Publication Date: 2019-04-05
JIANGSU WECARE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most of the fermented products of probiotic enterprises are obtained by expanding the seeds of the strains, fermenting, centrifuging and collecting the bacteria, and then freeze-drying or spray-drying to obtain bacte

Method used

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  • Preparation method of probiotics additive applied for livestock breeding
  • Preparation method of probiotics additive applied for livestock breeding
  • Preparation method of probiotics additive applied for livestock breeding

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0031] Experimental example 1 Collection of fermentation supernatant

[0032] Although the fermentation method of each probiotic is different, the subsequent processing method of the fermentation supernatant used in animal husbandry is not much different. Therefore, this embodiment only takes Lactobacillus plantarum as an example for further explanation and provides a probiotic The specific steps of fermentation and collection of fermentation supernatant are as follows:

[0033] S1. Take a glycerol tube of Lactobacillus plantarum strain, and inoculate it into MRS medium (a test tube with a liquid volume of 9ml), at 35-37°C, anaerobic static culture for 18-24h, named System 1.

[0034] S2. Take system 1 and inoculate it into MRS medium (200-400ml filling amount) with an inoculum amount of 1-2%, incubate anaerobic statically at 35-37°C for 14-18h, and name it system 2.

[0035] S3. Take system 2 and inoculate it in MRS medium (10-20L) with an inoculum amount of 1-2%, at 35-37°C, anaerob...

Example Embodiment

[0038] Experimental example 2 Treatment of fermentation supernatant

[0039] This example uses the fermentation supernatant obtained in Example 1 to further provide a comparative test method for processing the fermentation supernatant and finally preparing it as a probiotic additive, which is specifically as follows:

[0040] 1. The choice of pH

[0041] Researchers in the field should understand that microorganisms are not easy to grow under lower pH conditions. However, for different solutions, due to different components, the optimal pH for inhibiting the growth of microorganisms is not the same. For this reason, in this example, the fermentation supernatant was placed at room temperature for a certain period of time under different pH conditions to detect the mold yeast and the total number of bacteria in the solution. Under different pH values, the mold yeast was 50 CFU. / ml (refer to GB 478915-2010 for counting method, the same below), the total number of bacteria is 1×10 3 Th...

Example Embodiment

[0073] Experiment 3 Application of probiotic fermentation supernatant

[0074] 1. Experimental animals

[0075] Experimental animals: According to the principles of the same genetic background, similar body weight, and the same litter type, the "Du×Chang×Large" ternary hybrid pigs weighing about 16-18kg are selected.

[0076] 2. Test materials

[0077] 1. Probiotic fermentation supernatant

[0078] The probiotic fermentation supernatant prepared by the methods of Examples 1 and 2 was selected.

[0079] 2. Test diet

[0080] The diet formula includes corn 55.24%, barley 7%, expanded soybean 6.23%, dehulled soybean meal 20.68%, secondary meal 6%, cotton meal 4%, soybean oil 0.5%, salt 0.35%, vitamins and trace elements .

[0081] In the diet of the control group, the diet and water were mixed and fed in a ratio of 1:2 to 4; in the diet of the test group, the diet and the probiotic fermentation supernatant were also mixed in a ratio of 1:2 to 4 , Feeding.

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Abstract

The invention relates to the field of livestock breeding feed additives, in particular to a preparation method of a probiotics additive applied for livestock breeding. The method comprises the steps of conducing pH value adjustment on probiotics fermentation supernate, adding a stabilizer, adding an antioxidant, adding prebiotics and conducting sterilization treatment, wherein the probiotics fermentation supernate is a whole fermentation post-treatment solution which comprises fermentation supernatant of probiotics and the probiotics. According to the preparation method of the probiotics additive applied for livestock breeding, the probiotics additive applied for livestock breeding is stable in storage, has an obvious effect of promoting the growth performance of hogs when applied to feed,and is not only environmentally friendly, but also economical.

Description

technical field [0001] The invention relates to the field of animal husbandry feed additives, in particular to a preparation method of a probiotic additive applied to animal husbandry. Background technique [0002] As a kind of intestinal microorganisms, probiotics can regulate the balance of intestinal flora, inhibit harmful bacteria, improve host immunity, and promote digestion and absorption for the host. Its mechanism of action is mainly due to the beneficial regulatory effect of the metabolites produced during its growth, such as lactic acid, bacteriocin, and exopolysaccharides, on the host. [0003] Most of the fermented products of probiotic enterprises are obtained by expanding the seeds of the strains, fermenting, centrifuging and collecting the bacteria, and then freeze-drying or spray-drying to obtain bacterial powder. The supernatant after centrifugation is usually directly used as waste water. Discharge, the processing process is not only cumbersome, but also r...

Claims

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Application Information

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IPC IPC(8): A23K50/30A23K10/12A23K20/105A23K20/20A23K20/121A23K20/126A23K20/163A23K10/18
CPCA23K10/12A23K10/18A23K20/105A23K20/121A23K20/126A23K20/163A23K20/20A23K50/30A23V2400/133A23V2400/137A23V2400/145A23V2400/147A23V2400/143A23V2400/151A23V2400/125A23V2400/123A23V2400/113A23V2400/165A23V2400/181A23V2400/173A23V2400/175A23V2400/169A23V2400/513A23V2400/517A23V2400/519A23V2400/529A23V2400/533
Inventor 方曙光严涛朱建国陈珂可夏九学
Owner JIANGSU WECARE BIOTECHNOLOGY CO LTD
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