A method for efficiently removing butyl xanthate and heavy metal cr(vi) in flotation wastewater by biological method
A technology for flotation wastewater and butyl xanthate, which is applied in the field of biological treatment of environmental pollutants, can solve the problems of unseen and unseen Shewanellaoneidensis MR-1 bioremediation of butyl xanthate, heavy metal Cr complex pollution, etc. The method is simple and important Environmental benefits and social value, the effect of good application prospects
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Embodiment 1
[0038] Embodiment 1: a kind of biological method efficiently removes the method for butyl xanthate and heavy metal Cr(VI) in flotation wastewater, comprising the steps:
[0039] (1) Strain activation culture:
[0040] Dissolve 0.005 g of purchased Shewanella oneidensis MR-1 freeze-dried product with 2 mL of LB medium (the composition of LB medium: 10 g of peptone, 5 g of yeast extract, and 10 g of sodium chloride dissolved in 1 L of distilled water, the same below) for activation, After obtaining the activated bacterial liquid, add 1.5 mL of the activated bacterial liquid to a conical flask containing 150 mL of LB medium, place it in a constant temperature shaker at 30°C and 150 rpm, and cultivate it until the logarithmic growth phase, and then centrifuge at 8000 r / min 5min to get the wet bacteria, and then wash twice with 10mL, 20mM sterilized HEPES solution, then disperse the washed wet bacteria in 10mL sterilized inorganic salt medium to obtain a suspension, and place the s...
Embodiment 2
[0045] Embodiment 2: a kind of biological method efficiently removes the method for butyl xanthate and heavy metal Cr(VI) in flotation wastewater, comprising the steps:
[0046] Change the initial concentration of butyl potassium xanthate and Cr(Ⅵ) in the step (2) of Example 1, and keep other operations and dosages unchanged, and investigate the effect of Shewanella oneidensis MR-1 on different concentrations of butyl potassium xanthate and Cr(Ⅵ). The removal effect, the specific results are as follows:
[0047] 1. When the initial concentrations of butyl potassium xanthate and Cr(Ⅵ) were 10 mg / L and 10 mg / L respectively, the removal rate of butyl potassium xanthate was calculated to be 100% by taking samples at 48 hours; after continuing to cultivate for 3 days, sampling and analysis And the reduction rate of Cr(Ⅵ) was calculated to be 100%.
[0048] 2. When the initial concentrations of butyl potassium xanthate and Cr(Ⅵ) were 20 mg / L and 10 mg / L respectively, the removal ra...
Embodiment 3
[0054] Embodiment 3: a kind of biological method efficiently removes the method for butyl xanthate and heavy metal Cr(VI) in flotation wastewater, comprising the steps:
[0055] Take 150mL of flotation wastewater from a tailings pond of a lead-zinc mine in Huangshi into a 250mL blue-mouth bottle, first adjust the pH of the wastewater to 7 with dilute hydrochloric acid, and then add an appropriate amount of KH 2 PO 4 、K 2 HPO 4 , NH 4 Cl, Na 2 SO 4 , MgSO 4 ·7H 2 O and HEPES make their respective concentrations 2.72g / L, 4.26g / L, 0.53g / L, 0.113g / L, 0.113g / L (referring to MgSO 4 ), 7.14g / L, then add the suspension liquid prepared in Example 1 step (1), so that the bacterial density OD 600 = 0.3, sealed with gauze and placed in a constant temperature shaker at 30°C and 150rpm for 48 hours to obtain a degradation solution, sampled 10 mL at the 48th hour to analyze the concentration of butyl xanthate in the supernatant, and calculate the removal of butyl potassium xanthate ...
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