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Cyclodextrin graft compound rubber beads and its application in biotransformation

A cyclodextrin and graft technology, applied in the field of biocatalysis, can solve problems such as not yet visible

Active Publication Date: 2021-09-21
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] As a natural polymer carrier, chitosan contains -OH and -NH in the molecule 2 The two groups are easy to modify, and glutaraldehyde can be used as a cross-linking agent to graft cyclodextrin. At the same time, sodium alginate can use epichlorohydrin as a cross-linking agent to graft cyclodextrin to make grafting The branching rate has been effectively improved, but the technology of making two kinds of grafts into composite rubber beads as a solubilizer for the biotransformation of insoluble compounds and recycling cyclodextrin has not yet been seen

Method used

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  • Cyclodextrin graft compound rubber beads and its application in biotransformation
  • Cyclodextrin graft compound rubber beads and its application in biotransformation

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Embodiment 1: a kind of cyclodextrin grafted compound glue beads and its incortisone acetate C 1,2 Applications in dehydrogenation reactions

[0038] 1. Preparation of composite rubber beads:

[0039] To prepare chitosan-β-cyclodextrin grafts, 1 g of chitosan was dissolved in 50 mL of 0.1 mol / L HCl solution, added to 50 mL of β-CD aqueous solution (6 g of β-CD), and stirred at 25° C. for 1 hour. Then, 6 mL of 25% glutaraldehyde solution was gradually added within 30 minutes, and after reacting in a water-bath shaker at 70° C. for 6 hours, 35 mL of 1 mol / L NaOH aqueous solution was added to the reaction solution to precipitate the product. The precipitate was rinsed thoroughly with water and acetone, and dried to constant weight.

[0040] To prepare sodium alginate-β-cyclodextrin graft, add sodium alginate (1g) and β-cyclodextrin with a molar ratio of 1:0.7 into a 100mL Erlenmeyer flask, add about 50mL of distilled water, and place in a water bath at 60°C Stir electri...

Embodiment 2

[0065] Embodiment 2: a kind of cyclodextrin grafted compound glue beads and its incortisone acetate C 1,2 Applications in dehydrogenation reactions

[0066] Except the following content, other is the same as embodiment 1.

[0067] Preparation of composite beads:

[0068] Prepare chitosan-HP-β-CD graft, 1g chitosan is dissolved in 50mL0.1mol / LHCl, solution is added in 50mLHP-β-CD aqueous solution (6.5g HP-β-CD), and at 25 ℃ Stir for 1 hour. Then, 5mL of 25% glutaraldehyde solution was gradually added within 30 minutes, and the reaction was reacted in a water-bath shaker at 50°C for 8 hours, and 35mL of 1mol / L NaOH aqueous solution was added to the reaction solution to precipitate the product. The precipitate was rinsed thoroughly with water and acetone, and dried to constant weight.

[0069] To prepare sodium alginate-HP-β-CD grafts, add sodium alginate (1g) and HP-β-CD with a molar ratio of 1:1.5 into a 100mL Erlenmeyer flask, add about 50mL of distilled water, and place i...

Embodiment 3

[0086] Embodiment 3: a kind of cyclodextrin grafted compound glue beads and its incortisone acetate C 1,2 Applications in dehydrogenation reactions

[0087] Except the following content, other is the same as embodiment 1.

[0088] Preparation of composite beads:

[0089]Prepare chitosan-RM-β-cyclodextrin graft, 1g chitosan is dissolved in 50mL0.1mol / L HCl, the solution is added in 50mL RM-β-CD aqueous solution (6g RM-β-CD), and Stir at 25°C for 1 hour. Then, 6mL of 25% glutaraldehyde solution was gradually added within 30 minutes, and the reaction was reacted in a water-bath shaker at 90°C for 2 hours, and 35mL of 1mol / L NaOH aqueous solution was added to the reaction solution to precipitate the product. The precipitate was rinsed thoroughly with water and acetone, and dried to constant weight.

[0090] To prepare sodium alginate-RM-β-cyclodextrin graft, add sodium alginate (1g) and RM-β-CD with a molar ratio of 1:1.5 into a 100mL Erlenmeyer flask, add about 50mL of distil...

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Abstract

The invention belongs to the technical field of biocatalysis, and in particular relates to a cyclodextrin graft composite rubber bead and its application in biotransformation. The invention provides a composite rubber bead with high cyclodextrin immobilization capacity and many times of recycling. The grafts are mixed in proportion and dropped into calcium chloride solution to form balls, which effectively increases the immobilization capacity of cyclodextrin, and the grafting rate of cyclodextrin of composite rubber beads is higher than that of sodium alginate cyclodextrin Branches are increased by 60-70%, 20%-40% higher than chitosan cyclodextrin grafts; the reaction time is shortened, and the conversion rate of steroid substrates is still above 90% after 10 cycles, achieving the improvement of steroid While improving the catalytic reaction efficiency of the body, it increases the utilization rate of the cyclodextrin and reduces the production cost, and has good application value and promotion prospect.

Description

Technical field: [0001] The invention belongs to the technical field of biocatalysis, and in particular relates to a cyclodextrin graft composite rubber bead, its preparation and its application in biotransformation. Background technique: [0002] As the second largest class of drugs after antibiotics, steroids have the function of regulating the metabolism and physiological effects of various substances in the living body. Compared with the traditional chemical synthesis method, the method of microbial transformation can form a variety of active intermediates of steroidal drugs with high purity. As a solubilizer, cyclodextrin can increase the solubility of hydrophobic steroids, and its special cavity structure can include steroid substrates, thereby improving the bioavailability and yield of steroid compounds. However, the high price of cyclodextrin restricts its wide application in the field of biocatalysis. By immobilizing cyclodextrin on a polymer carrier through graft...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/12C08L87/00C08G81/00C12N11/10C12P33/00
CPCC08G81/00C08J3/12C08J2387/00C08J2487/00C12N11/10C12P33/00
Inventor 申雁冰王敏牛璐璐骆健美夏梦雷耿宇涵郭俊超
Owner TIANJIN UNIV OF SCI & TECH