Differentially expressed NACs transcription factor in populus tomentosa under cadmium stress condition as well as obtaining method thereof

A transcription factor and differential expression technology, applied in the field of bioinformatics, can solve problems such as affecting crop yield and quality, unclear gene expression regulation mechanism, and plant damage.

Active Publication Date: 2019-04-09
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a non-essential metal element for plant growth and development, cadmium may cause harm to plants when the content is low, such as reducing the activity of enzymes and photosynthetic intensity in plants, causing metabolic disorders, changing the permeability of cell membranes, hindering root growth, and inhibiting its growth. Absorption of water and nutrients, affecting crop yield and quality
But so far, the regulatory mechanisms of many gene expressions under heavy metal cadmium stress are still unclear.

Method used

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  • Differentially expressed NACs transcription factor in populus tomentosa under cadmium stress condition as well as obtaining method thereof
  • Differentially expressed NACs transcription factor in populus tomentosa under cadmium stress condition as well as obtaining method thereof
  • Differentially expressed NACs transcription factor in populus tomentosa under cadmium stress condition as well as obtaining method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Obtaining candidate NACs transcription factors differentially expressed in Populus tomentosa under cadmium stress

[0060] The excellent clone plant "1316" [P.tomentosa Carr.(clone'1316')] of Populus tomentosa in that year was used as the experimental material. °47′). Select the young shoots of the excellent clone plants that were born in January to graft in January, and select plants with good growth and basically the same in July of that year to process, that is, to use six-month-old seedlings for experimentation. First the seedlings are taken out from the soil, washed with water and carefully cleaned up the roots, then put into an aqueous solution containing 20 mg / L of cadmium chloride for processing, and after 6 hours, take leaf samples, each sample contains three biological repetitions, after sampling Immediately freeze in liquid nitrogen.

[0061] The total RNA of leaves was extracted with RNAplant Plus Plant Total RNA Extraction Reagent, and the operation steps...

Embodiment 2

[0067] Obtain SNP data of differentially expressed candidate NACs transcription factors

[0068] Genome sequences of 435 Populus tomentosa populations were determined by genome resequencing technology. The genome sequence obtained by sequencing was compared with the genes in the P. tomentosa germplasm resource bank to obtain the genome SNP data of P. tomentosa treated with cadmium stress. Then screen the SNP sites with a minimum allele frequency (Minor allele frequency, MAF) above 5% and missing data within 20%, and finally obtain high-quality SNP data.

[0069] Based on the population resequencing data, 20 differentially expressed candidate NACs transcription factors were mapped to the corresponding P. tomentosa genes by multiple alignment method, and SNP data were extracted. A total of 889 SNP sites from 20 NACs transcription factors were obtained.

Embodiment 3

[0071] The 435 Populus tomentosa natural populations adopted in Example 2 were tested for phenotypic traits, including the following 10 population phenotype data related to growth and photosynthesis: tree height (Height, H), diameter at breast height (Diameter atbreast height, D) and Stem volume (V), net photosynthetic rate (Net photosythetic rate, Pn), stomatal conductance (Sromatal conductance, SC), transpiration rate (Transpiration rate, Tr), intercellular CO 2 Concentration (IntercellularCO 2 concentration, Ci), malondialdehyde content (Malondialdehyde Content, MDA) and total protein content (Total Protein Content, P).

[0072] The diameter at breast height, tree height, and volume were measured by the growth traits measurement tool, and the net photosynthetic rate, stomatal conductance, transpiration rate, and intercellular CO were measured by the LI6400 photosynthetic measuring instrument. 2 Concentration of four photosynthetic traits, using kits to determine malondiald...

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Abstract

The invention belongs to the technical field of bioinformation and provides a differentially expressed NACs transcription factor in populus tomentosa under the cadmium stress condition as well as an obtaining method thereof. The differentially expressed NACs transcription factor in the populus tomentosa under the cadmium stress condition is obtained by a high-throughput sequencing technology and abioinformatic analysis method. The differentially expressed NACs transcription factors in the populus tomentosa under the cadmium stress condition are obtained for the first time through transcriptome analysis and association analysis combined strategy, the transcription factors can influence the growth and photosynthesis of the populus tomentosa under the cadmium stress condition, and importantreference value and theoretical significance in treating and repairing heavy metal pollution as well as improving the stress resistance of plants are achieved.

Description

technical field [0001] The invention belongs to the technical field of bioinformatics, and in particular relates to a NACs transcription factor in Populus tomentosa under the condition of cadmium stress and an acquisition method thereof. Background technique [0002] Soil is the material basis for human survival, and it is also the earliest agricultural production resource developed and utilized by human beings. It is closely related to many problems such as food, resources and the environment that human beings face all over the world today. Cadmium is one of the heavy metals that cause the largest area of ​​soil pollution and the most serious harm. As a non-essential metal element for plant growth and development, cadmium may cause harm to plants when the content is low, such as reducing the activity of enzymes and photosynthetic intensity in plants, causing metabolic disorders, changing the permeability of cell membranes, hindering root growth, and inhibiting its growth. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B25/00G16B30/00
Inventor 张德强刘鑫陈盼飞
Owner BEIJING FORESTRY UNIVERSITY
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