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Construction and application of PEI-AuNPs-ZnPP (II) high-sensitivity H2O2 detection electrochemical sensor

A technology of protoporphyrin zinc and H2O2, applied in the direction of material electrochemical variables, scientific instruments, nanotechnology, etc., can solve the problems of long analysis time, high detection cost, low detection limit, etc., and achieve good chemical stability and biological The effect of good compatibility and low detection limit

Active Publication Date: 2019-04-12
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of these methods have disadvantages such as high detection cost, complicated operation, long analysis time, and low detection limit.

Method used

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  • Construction and application of PEI-AuNPs-ZnPP (II) high-sensitivity H2O2 detection electrochemical sensor
  • Construction and application of PEI-AuNPs-ZnPP (II) high-sensitivity H2O2 detection electrochemical sensor
  • Construction and application of PEI-AuNPs-ZnPP (II) high-sensitivity H2O2 detection electrochemical sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1: Synthesis of nano-gold-protoporphyrin zinc (II) (PEI-AuNPs-ZnPP) biomimetic enzyme:

[0048] (1) Weigh 2.06g of polyethyleneimine (PEI) and 340μL of 2% chloroauric acid (HAuCl 4 ) solution, then add 1960mL ultrapure water, heat up to 80°C intermittently while stirring, keep it warm, observe the color change of the solution, when the solution turns light ruby ​​red, stop heating, continue stirring to room temperature, and obtain nano-gold (PEI-AuNPs) solution, stored at 4°C in the dark for future use;

[0049] (2) dissolving zinc protoporphyrin (II) (ZnPP) in N,N-dimethylformamide (DMF) to prepare a ZnPP solution with a concentration of 0.1 mmol / L;

[0050] (3) Mix 4.8mL ZnPP solution with 4mL nano-gold solution, and stir the mixture on a magnetic stirrer for 1h;

[0051] (4) After stirring, transfer the mixture to a centrifuge tube, and centrifuge at 14000 rpm for 80 min at 4°C;

[0052] (5) After centrifugation, discard the supernatant, then add 1.0 mL...

Embodiment 2

[0065] Embodiment 2: the preparation of electrochemical sensor

[0066] (1) Electrode (GCE) cleaning:

[0067] On the polishing plate, use alumina polishing powder with a particle size of 0.3 μm to polish the glassy carbon electrode to the mirror surface, and then insert the glassy carbon electrode into 5 mmol / L potassium ferricyanide solution for linear cyclic voltammetry scanning test (scanning range: - 0.2V~0.6V, scan rate: 0.05V / s, sampling interval 1mV; static time: 5s, cathode current is positive, and the redox peak potential difference of the glassy carbon electrode should be less than 80mV when the electrode treatment is considered qualified. If it is not qualified , then repeat the above operation). Put the qualified glassy carbon electrode into an ultrasonic cleaner, wash it with ultrapure water, absolute ethanol, and ultrapure water for 1 min, and finally dry it with nitrogen.

[0068] (2) Electrode modification:

[0069] Add 4-6 μL of nano-gold-protoporphyrin zi...

Embodiment 3

[0070] Embodiment 3: Feasibility detection of electrochemical sensor

[0071] 5 μL of gold nanoparticles (PEI-AuNPs), zinc protoporphyrin (II) (ZnPP), gold nanoparticles-zinc protoporphyrin (II) (PEI-AuNPs-ZnPP) were respectively modified on the surface of the electrode (the method was the same as in Example 2 ), and the bare electrode, vacuum-dry for 30min, add 10μL 0.5mol / L H 2 o 2 , measured by differential pulse voltammetry (scanning range: 0V ~ -1V), the results are as follows Figure 4 , the current signal of nano-gold-protoporphyrin zinc (Ⅱ) is the strongest, which proves that the method is feasible.

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Abstract

The invention discloses construction and application of a PEI-AuNPs-ZnPP (II) high-sensitivity H2O2 detection electrochemical sensor, and particularly relates to synthesis of PEI-AuNPs-ZnPP (II) and detection of PEI-AuNPs-ZnPP (II) to H2O2 in human serum. AuNPs and ZnPP can be synthesized through the characteristic of amido bond connection, the AuNPs and ZnPP are modified on the surface of a glassy carbon electrode, the H2O2 concentration is detected through an electrochemical method, the results show that under the optimal conditions, the concentration of H2O2 is positively correlated with the current signal with the certain range, the linear equation is I([mu]A)=0.20412c (pmol / L)+1.93557, the correlation coefficient is 0.99534, and the detection limit is 8.540*10<-13>M; and the stabilityof the electrochemical sensor is good and is embodied in that the current signal of the sensor after three days is 96.8% of an initial value, meanwhile, the sensor also shows the high recovery rate (97.57 to 101.43%) in H2O2 measuring in actual sample human serum, and can be used for detecting the H2O2 concentration, and a foundation is laid for later application to detection of the concentrationof glucose in the medical field.

Description

technical field [0001] The invention belongs to the field of construction and application of electrochemical sensors, in particular to the synthesis of nano-gold-protoporphyrin zinc (II) (PEI-AuNPs-ZnPP) and its effect on H in human serum. 2 o 2 The detection belongs to the technical field of bioanalysis. Background technique [0002] Hydrogen peroxide (H 2 o 2 ) as an important biomarker, widely used in food production, environmental monitoring, drug synthesis and clinical testing and other fields, hydrogen peroxide is a toxic by-product produced in the process of human metabolism, which can cause great damage to the body, the study found h 2 o 2 Overdose can cause Alzheimer's, Parkinson's and other central nervous system disorders; therefore H 2 o 2 Sensitive detection of content is of great significance in the fields of physiology, pathology and environment. At present, there are many methods for the determination of hydrogen peroxide content, such as colorimetry,...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/416G01N27/48B82Y30/00
CPCB82Y30/00G01N27/308G01N27/4163G01N27/48
Inventor 杨怀霞刘艳菊李晓飞李金鸽李曼曼郭文峰赵可欣
Owner HENAN UNIV OF CHINESE MEDICINE
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