Alcohol-free large-tonifying beverage extracted through composite enzymatic hydrolysis and preparing method thereof

A technology of compound enzymatic hydrolysis and extraction method, which is applied in the direction of food ingredients containing natural extracts, food ingredients, and the functions of food ingredients, etc., to achieve the effect of high extraction rate, high extraction rate, and good substrate specificity

Inactive Publication Date: 2019-04-19
JINAN HANGCHEN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the method of extracting the active ingredients in the three substances of Cordyceps sinensis, Ganoderma lucidum and velvet antler at one time by enzymatic hydrolysis in a process flow

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The velvet antlers are depilated, washed, and dried separately at low temperature with the mycelium of Cordyceps fungus and the spore powder of Ganoderma lucidum. According to parts by weight, 5 parts of dried Cordyceps mycelia and 3 parts of velvet antler are respectively pulverized to less than 36 meshes, and fully mixed with 2 parts of Ganoderma lucidum spore powder. Mix the dry powder with an appropriate amount of water, and ultrasonically break the wall at 35°C for 60 minutes, and the wall breaking rate is 92%.

[0028] The broken wall mixture was replenished with water until the solid-to-liquid ratio was 1:10, mixed evenly, adjusted to pH 5.5 with malic acid, and stirred at 45°C at a constant temperature of 150 rpm for 45 minutes for enzymatic extraction. The non-protease used for enzymolysis is a mixture of the following three in parts by weight:

[0029] Cellulase 0.2 %

[0030] Pectinase 0.1 %

[0031] Laccase 0.1 %

[0032] After the above enzymatic hydrol...

Embodiment 2

[0040] The velvet antlers are depilated, washed, and dried separately at low temperature with the mycelium of Cordyceps fungus and the spore powder of Ganoderma lucidum. According to parts by weight, 10 parts of dried Cordyceps mycelium and 8 parts of velvet antler are respectively pulverized to less than 36 meshes, and fully mixed with 4 parts of Ganoderma lucidum spore powder. Mix the dry powder with an appropriate amount of water, and ultrasonically break the wall at 50°C for 80 minutes, and the wall breaking rate is 94%.

[0041] The broken wall mixture was replenished with water until the solid-to-liquid ratio was 1:20, mixed evenly, adjusted to pH 4.8 with malic acid, and stirred at 60°C at a constant temperature of 200 rpm for 60 minutes for enzymatic extraction. The non-proteases used for enzymolysis are the following mixtures in parts by weight:

[0042] Cellulase 0.4 %

[0043] Glycosidase 0.3 %

[0044] Mannanase 0.3 %

[0045] Xylanase 0.3 %

[0046] After the...

Embodiment 3

[0055] The velvet antlers are depilated, washed, and dried separately at low temperature with the mycelium of Cordyceps fungus and the spore powder of Ganoderma lucidum. According to parts by weight, 6 parts of dried Cordyceps mycelium and 3 parts of velvet antler are respectively pulverized to less than 36 meshes, and fully mixed with 4 parts of Ganoderma lucidum spore powder. Mix the dry powder with an appropriate amount of water, and ultrasonically break the wall at 45°C for 100 minutes, and the wall breaking rate is 94%.

[0056] The broken wall mixture was replenished with water until the solid-to-liquid ratio was 1:15, mixed evenly, adjusted to pH 5.5 with malic acid, stirred at 55°C at a constant temperature of 160 rpm for 50 minutes for enzymatic extraction. The non-proteases used for enzymolysis are the following mixtures in parts by weight:

[0057] Cellulase 0.4 %

[0058] Glycosidase 0.2 %

[0059] Pectinase 0.2 %

[0060] Laccase 0.3 %

[0061] After the abov...

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PUM

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Abstract

The invention belongs to the field of health care products, and particularly relates to an alcohol-free large-tonifying beverage extracted through medium-and-low-temperature composite enzymatic hydrolysis and a preparing method thereof. The alcohol-free large-tonifying beverage is formed by evenly mixing concentrated liquor of cordyceps sinensis, lucid ganoderma and corn cervi pantotrichum and nourishing-auxiliary-material extracting liquid according to a certain proportion. According to the alcohol-free large-tonifying beverage extracted through medium-and-low-temperature composite enzymatichydrolysis and the preparing method thereof, water serves as a solvent, three raw materials of cordyceps sinensis, lucid ganoderma and corn cervi pantotrichum are mixed and subjected to wall breaking,and then the alcohol-free large-tonifying beverage is extracted with the biological enzymolysis method with the mild and efficient technology condition in a same system. In the extracting process, dual-step compound enzymic hydrolysis through non-protease and protease is conducted, the obtained extracted does not contain alcohol substances, bioactive constituents in the raw materials are well reserved. The compound biological-activity health care beverage prepared with the method has high nourishing ingredients and a healthcare function, and is suitable for recuperating persons, old people and weakness-due-to-chronic-disease persons to take.

Description

technical field [0001] The invention belongs to the field of health care products, and in particular relates to an alcohol-free tonic drink extracted by compound enzymatic hydrolysis at a medium and low temperature and a preparation method thereof. Background technique [0002] Cordyceps, Ganoderma lucidum, and velvet antler are nourishing Chinese herbal medicines with high nutritional content. They have multiple biological activities and high nutritional value, and are common nourishing health products. However, for the above three precious medicinal materials, traditional extraction methods are often based on ultrasonic vibration, high-temperature cooking, acid hydrolysis and alcohol immersion. Some of the above reaction conditions are relatively harsh, resulting in a large loss of bioactive components such as amino acids and vitamins; some have a low extraction rate, and the nutritional components cannot be fully retained through a single method of extraction; some produc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L2/38A23L2/84A23L33/105A23L31/00A23L33/10
CPCA23L2/38A23L2/84A23L31/00A23L33/10A23L33/105A23V2002/00A23V2200/30A23V2250/21A23V2250/208A23V2250/204
Inventor 岳秋林赵林李昆仑李宝君孙欣
Owner JINAN HANGCHEN BIOTECHNOLOGY CO LTD
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