Regenerated tissue matrix composition for micro-surgery, preparation and application

A matrix composition and technology for regenerating tissue, applied in the field of regenerative medicine, can solve the problems of easily producing antigenicity, and achieve the effect of easy cell adhesion and migration, and excellent filling effect

Active Publication Date: 2019-04-19
北京颢美细胞基因生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Heterogeneous collagen, easy to produce antigenicity and other disadvantages

Method used

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  • Regenerated tissue matrix composition for micro-surgery, preparation and application
  • Regenerated tissue matrix composition for micro-surgery, preparation and application
  • Regenerated tissue matrix composition for micro-surgery, preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] 1) The raw material of allograft skin is put into 0.5-5% alkali glycerin solution, soaked at 35°C for 10 hours and shaken to obtain semi-finished product A;

[0052] 2) After removing the epidermis of the semi-finished product A, wash it with sterile pure water for 5 minutes, put it into a 0.3g / L SDS surfactant solution, ultrasonicate for 5 minutes at an ultrasonic frequency of 45KHz, and an ultrasonic temperature of 20°C, soak for 3 hours, and replace it with a new one Surfactant solution, repeat the treatment 3 times to get the semi-finished product B.

[0053] 3) Rinse the semi-finished product B with sterile pure water for 5 minutes, put it into 0.6% genipin water solution with a pH of 6.5 for cross-linking for 24 hours, and rinse it with sterile pure water for 5 minutes.

[0054] 4) Cut the semi-finished product B after step 3) to a size of (0.3~1)×(0.3~1)cm 2, immersed in liquid nitrogen for 10 min in an immersion manner to obtain a semi-finished product C.

[0...

Embodiment 2-5

[0060] The steps are the same as in Example 1, and the specific parameters are shown in Table 1.

[0061] Each parameter in table 1 embodiment 1-5 test procedure

[0062]

[0063]

[0064] Explanation: The particle size of the finished product that can pass through No. 14 and No. 20 sieves.

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Abstract

The invention provides a regenerated tissue matrix composition for micro-surgery, preparation and application. The reconstituted tissue matrix composition comprises regenerated tissue matrix microparticles, hydroxyethyl starch and carboxymethylcellulose; the regenerated tissue matrix microparticles contain an extracellular matrix in a microparticle state, and can pass through a 100-mesh screen, but cannot pass through a 400-mesh sieve. The regenerated tissue matrix composition has a three-dimensional structure and good biocompatibility, thereby being capable of serving as a stent for tissue supporting and cell growth and serving as a filler for facial micro-surgery. A minimally invasive injection product comprising the composition causes no immunological rejection in clinical use, is suitable for being injected into the dermis layer to repair facial wrinkles caused by the reduction of dermal collagen and elastic fiber or modify defective skin, and has a good tissue repair effect.

Description

technical field [0001] The invention belongs to the field of regenerative medicine, and specifically relates to a composition containing a regenerative tissue matrix, a preparation method and application thereof. Background technique [0002] Micro-surgery is a high-tech medical technology. It does not require surgery and has the characteristics of becoming beautiful and younger in a short time. It has gradually replaced the plastic surgery in the past. It is safe, without wounds, and recovers Advantages of short term. The emergence of micro-plastic surgery makes plastic surgery as simple as cosmetic skin care, and the plastic surgery effect is exactly the same as traditional plastic surgery. Micro-plastic surgery includes many types of items, and its main implementation methods include Botox type A wrinkle removal and face-lifting, filler injection, autologous fat grafting, facial liposuction, laser treatment, injection of hyaluronic acid and whitening injections, etc. The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/20A61L27/50A61L27/56
CPCA61L27/20A61L27/3633A61L27/3691A61L27/50A61L27/56A61L2400/06C08L1/286C08L3/08
Inventor 莫丽影孙善凤石卫华王姣王正元
Owner 北京颢美细胞基因生物技术有限公司
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