Detection method of procymidone in vegetables

Abstract

The invention provides a detection method of procymidone in vegetables. The detection method comprises the following steps of (1) extracting articles to be tested to obtain extraction liquid; (5) adding decoloring agents into extraction liquid obtained in the step (1) for decoloring; obtaining a solution obtained after the decoloring; (3) performing ring opening reaction on the obtained decoloredsolution under the effect of alkali to obtain ring opening compounds; performing condensation reaction on the ring opening compounds under the effects of hydroxylamine to obtain hydroxamic acid compounds; (4) acidifying the hydroxamic acid compounds obtained in the step (3); adding a buffer solution for regulating pH; then, adding color developing agents for color development. The procymidone in the vegetables is detected by a visual colorimetric method. The detection method has the advantages that the detection method is simple; the analysis period is short; the cost is low; the application range is wide; the data accuracy is high; the method can be used for procymidone detection, and meets the production requirements of enterprises.

Description

technical field [0001] The invention belongs to the field of analytical chemistry and relates to a method for detecting procymidone in vegetables. Background technique [0002] Procymidone is a systemic fungicide with protective and therapeutic effects. It is used for vegetable crops such as rapeseed, radish, eggplant, and leek to prevent gray mold and sclerotinia. The effect is obvious under low temperature and high humidity conditions. According to GB2763-2014 "National Food Safety Standard Maximum Residue Limits in Foods", the maximum residue limit of procymidone in leek is 0.2mg / kg. According to the survey, the main reason for the excessive residue of procymidone in chives is that the growers use this agent to prevent the occurrence of Botrytis cinerea. Chive gray mold is a disease that infects many times in one growing season. It mainly damages the leaves of leek. Typical symptoms include white spots, dry tips and wet diseases. A small amount of pesticide residues wil...

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Problems solved by technology

The substance to be tested was extracted with acetonitrile, homogenized at a high speed on a homogenizer, filtered, 5-7g of sodium chloride was salted out, 10.0mL of the filtrate was extracted and dried, dissolved in n-hexane, rinsed with Florisil column, and collected The lotion was dried to a constant volume and then analyzed by gas chromatography to realize the determination of procymidone residues; this method has the advantages of high sensitivity and low detection limit, but as a relatively expensive instrument, gas chromatography is not applicable in industrial production Screening of a large number of samples

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