Method for preparing rich xylobiose to xylotetraose from eucalyptus hydrothermal pretreatment liquid

A technology of pretreatment liquid and xylotetraose, which is applied in the field of biomass refining, can solve the problems of low utilization rate of resources, achieve the effects of reducing production costs, improving accessibility, and convenient and simple preparation process

Pending Publication Date: 2019-04-26
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the hardwood hydrothermal pretreatment liquid contains a large amount of xylooligosaccharides, and some enterprises mix and burn the pretreatment liquid and black liquor to generate heat, and the resource utilization rate is low

Method used

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  • Method for preparing rich xylobiose to xylotetraose from eucalyptus hydrothermal pretreatment liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] After the eucalyptus hydrothermal pretreatment liquid is centrifuged, the supernatant is taken, and xylooligosaccharides rich in xylobiose-xylotetraose are prepared according to the following steps:

[0041] (1) The eucalyptus wood hydrothermal pretreatment solution was treated with calcium hydroxide at room temperature and with a pH value of 3.9. Wherein, the dosage of calcium hydroxide is 1.0wt%, and the treatment time is 15min.

[0042] (2) Centrifuge the pretreatment solution after the calcium hydroxide treatment, and take the supernatant. Centrifugation speed 4500rpm, centrifugation time 5min.

[0043] (3) The supernatant obtained in the step (2) is subjected to activated carbon treatment at normal temperature. Wherein, the dosage of activated carbon is 0.8wt%, and the treatment time is 10min.

[0044] (4) Centrifuge the pretreatment solution after activated carbon treatment, and take the supernatant. Centrifugation speed 4500rpm, centrifugation time 5min.

[...

Embodiment 2

[0053] After the eucalyptus hydrothermal pretreatment liquid is centrifuged, the supernatant is taken, and xylooligosaccharides rich in xylobiose-xylotetraose are prepared according to the following steps:

[0054] (1) The eucalyptus wood hydrothermal pretreatment solution was treated with calcium hydroxide at room temperature and with a pH value of 3.9. Wherein, the dosage of calcium hydroxide is 1.0wt%, and the treatment time is 30min.

[0055] (2) Centrifuge the pretreatment solution after the calcium hydroxide treatment, and take the supernatant. Centrifugation speed 4500rpm, centrifugation time 5min.

[0056] (3) The supernatant obtained in the step (2) is subjected to activated carbon treatment at normal temperature. Wherein, the dosage of activated carbon is 0.8wt%, and the treatment time is 5min.

[0057] (4) Centrifuge the pretreatment solution after activated carbon treatment, and take the supernatant. Centrifugation speed 4500rpm, centrifugation time 5min.

[0...

Embodiment 3

[0066] After the eucalyptus hydrothermal pretreatment liquid is centrifuged, the supernatant is taken, and xylooligosaccharides rich in xylobiose-xylotetraose are prepared according to the following steps:

[0067] (1) The eucalyptus wood hydrothermal pretreatment solution was treated with calcium hydroxide at room temperature and with a pH value of 3.9. Wherein, the dosage of calcium hydroxide is 1.0wt%, and the treatment time is 20min.

[0068] (2) Centrifuge the pretreatment solution after the calcium hydroxide treatment, and take the supernatant. Centrifugation speed 4500rpm, centrifugation time 5min.

[0069] (3) The supernatant obtained in the step (2) is treated with activated carbon under normal temperature conditions. Among them, the dosage of activated carbon is 0.8%, and the treatment time is 5min.

[0070] (4) Centrifuge the hydrolyzate after activated carbon treatment, and get the supernatant. Centrifugation speed 4500rpm, centrifugation time 5min.

[0071] (...

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Abstract

The invention discloses a method for preparing rich xylobiose to xylotetraose from eucalyptus hydrothermal pretreatment liquid. The eucalyptus hydrothermal pretreatment liquid is sequentially treatedwith calcium hydroxide, activated carbon adsorption and endo-xylanase to obtain xylooligosaccharide-rich sugar liquid. Then, the sugar liquid is treated with membrane dialysis and freeze-dried to obtain xylooligosaccharide powder rich in xylobiose to xylotetraose. By means of the method of the invention, the operating cost of equipment in the process of refining xylooligosaccharide can be effectively reduced, the environmental pollution load can be alleviated, the yield and purity of the xylooligosaccharide can be increased, the high-value utilization of the hydrothermal pretreatment liquid isrealized, and the market price of the xylooligosaccharide is reduced to a certain extent.

Description

technical field [0001] The invention belongs to the field of biomass refining, and relates to a preparation method of xylobiose to xylotetraose, in particular to a preparation method of eucalyptus hydrothermal pretreatment liquid xylooligosaccharides combined with physics, chemistry and biology Background technique [0002] The statements herein merely provide background information related to the present invention and may not necessarily constitute prior art. [0003] Xylo-oligosaccharides (Xylo-oligosaccharides, XOS), also known as xylooligosaccharides, is a functional oligosaccharide composed of 2 to 7 xylose molecules linked by β-1,4 glycosidic bonds. Sugar has good physical and chemical properties, such as selective proliferation of intestinal probiotics; lowering cholesterol, improving calcium bioavailability; anti-oxidation, antibacterial, enhancing immunity, improving cardiovascular system, etc., especially xylobiose, Xylotriose and xylotetraose worked best. In add...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/12A23L33/125
CPCA23L33/125C12P19/12C12P19/14C12P2201/00C12P2203/00
Inventor 陈嘉川杨桂花吉兴香董吉冉徐丰
Owner QILU UNIV OF TECH
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