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Extruded papain time and temperature indicator

A technology of papain, time and temperature, which is applied in biochemical instruments, enzymology/microbiology devices, bioreactor/fermentation tank combinations, etc., can solve the problems of cold chain logistics chain breakage and lack, and achieve cost-friendly, enzymatic activity The effect of enhancing and speeding up the speed of irreversible color change

Active Publication Date: 2019-05-03
SHANGHAI OCEAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is a phenomenon of "broken chain" in cold chain logistics, so consumers lack sufficient trust in the food of cold chain logistics. The development of a TTI that can visualize and truly reflect the shelf life of food can greatly enhance consumers' confidence in cold chain food

Method used

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  • Extruded papain time and temperature indicator
  • Extruded papain time and temperature indicator
  • Extruded papain time and temperature indicator

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Embodiment Construction

[0032] In order to fully disclose a piercing neutral protease type time temperature indicator of the present invention, it will be described in conjunction with the accompanying drawings.

[0033] In the papain-type TTI reaction system, the amino acids (tryptophan and tyrosine, etc.) produced by papain decomposing the substrate soybean protein reduce the sodium tungstate and sodium molybdate in the Foline phenol-reagent solution B, resulting in a dark blue color of the system The color of the TTI system changes from light yellow to dark blue. Therefore, the color difference value b* is mainly used as the consideration index to determine the formula of each component, and the TTI formula is obtained by processing and analyzing the data obtained from the experiment. At the same time, the kinetic model is reasonably selected according to different food sciences, and combined with the reaction of TTI, the system It can reflect the law of the quality of food changing with time duri...

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Abstract

The invention relates to an extruded papain time and temperature indicator, which is composed of a combined container containing a color developing reagent, a substrate and enzyme; the color developing reagent is a Folin phenol reagent liquid B, and the concentration is 2 mg / L; the substrate is a mixture of 20 g / L of a soy protein phosphate buffer solution, 0.1 mol / L of a sodium hydroxide solutionand distilled water; the enzyme is a papain solution having a concentration of 2 mg / L. A chromogenic system of an enzyme-substrate reaction designed according to the method for determining the protein concentration of Folin-phenol, irreversible color change can be carried out under the passage of time or temperature fluctuation, so that a visible color change is generated, and the purpose of indicating the time experienced by the chilled food or the change in quality after the temperature change is achieved.

Description

technical field [0001] The invention belongs to the technical field of cold chain logistics, and relates to a time-temperature indicator capable of monitoring the quality state and freshness of food in the process of cold chain logistics in real time. Background technique [0002] Fresh aquatic food requires a low temperature environment during storage and transportation, and fluctuations in storage temperature will have an important impact on the safety of this type of food. In order to ensure the safety of food, it is necessary to effectively monitor the storage temperature of food. In this way, the principle of least-shelf-life / first-out LSFO can be implemented based on the actual quality of the food. [0003] Time-temperature indicator (Time-Temperature Indicator, TTI) is an indicator that reflects the temperature change process experienced by food through the cumulative effect of time and temperature, usually manifested as mechanical deformation or color change, which ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12Q1/37
Inventor 谢晶黄文博王金锋
Owner SHANGHAI OCEAN UNIV
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