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Ag/ZIF-8 anti-bacterial agent and preparation method thereof

A technology of ZIF-8 and antibacterial agent, which is applied in the field of Ag/ZIF-8 nano-bacteriostatic agent and its preparation, can solve problems such as easy aggregation of silver nanoparticles, poor stability, and limited antibacterial effect, and achieve good stability, The effect of mild synthetic conditions and broad antibacterial spectrum

Inactive Publication Date: 2019-05-17
DALIAN NATIONALITIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, silver nanoparticles have the disadvantages of easy aggregation and poor stability, which seriously limit their antibacterial effect in practical applications.

Method used

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  • Ag/ZIF-8 anti-bacterial agent and preparation method thereof
  • Ag/ZIF-8 anti-bacterial agent and preparation method thereof
  • Ag/ZIF-8 anti-bacterial agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 adopts impregnation-reduction method to prepare Ag / ZIF-8 nano antibacterial agent

[0031] S1. Synthesis of ZIF-8

[0032] Take 125mL deionized water and add it to the Erlenmeyer flask, add 1.88g Zn(NO 3 )·6H 2 O, 1.2665g CTAB, 2.1425g histidine were ultrasonically dissolved to obtain liquid A;

[0033] Take 125mL of deionized water and add it to another Erlenmeyer flask, add 4.074g of 2-methylimidazole and 6.8565ml of triethylamine for ultrasonic dissolution to obtain liquid B;

[0034] Pour liquid B into liquid A, stir and reflux for 7 hours at a temperature of 20-60°C. The volume ratio of methanol: deionized water = 1:1 was used to centrifuge and wash 3 times, and then refluxed 3 times with methanol, 40min each time, and centrifugally dried to obtain white powder ZIF-8.

[0035] Synthesis of S2.Ag / ZIF-8

[0036] Take 4ml of methanol and add to the Erlenmeyer flask, add 0.5g of ZIF-8, 0.01g of AgNO 3 React for 2 hours, then gradually add 89 mg of so...

Embodiment 2

[0038] Ag / ZIF-8 prepared in the embodiment 1 is evaluated by shake flask method to the bacteriostatic effect of Staphylococcus aureus (Gram-positive bacteria) and Escherichia coli (Gram-negative bacteria), test result is as follows image 3 shown.

[0039] Staphylococcus aureus and Escherichia coli were cultured in LB (Luria-Bertani) broth at 37°C for 12 hours. Disperse 0-12mgAg / ZIF-8 into 40ml LB liquid medium respectively, add 400μL concentration of 10 8 CFU / ml of Staphylococcus aureus and E. coli suspension. Bacterial growth rate was measured by monitoring the optical density at 600 nm while incubating at 37°C. image 3 (a) is the antibacterial effect of Staphylococcus aureus, image 3 (b) is the antibacterial effect of Escherichia coli; it can be clearly seen from the figure that after adding Ag / ZIF-8 to the liquid medium, the growth rate of bacteria can be significantly reduced.

Embodiment 3

[0041] The Ag / ZIF-8 nanometer bacteriostatic agent prepared in the plate method evaluation embodiment 1 is to the bacteriostasis of escherichia coli (gram-negative bacterium) and Bacillus subtilis (gram-positive bacterium), test result is as follows Figure 4 shown.

[0042] Disperse 0-12mgAg / ZIF-8 nanomaterials into 40ml agar medium, and then 50μL concentration of 10 5 The bacterial suspension of CFU / ml was evenly spread on the agar plate, and then cultured at 37° C. for 12 hours, and the number of bacterial colonies was observed. Figure 4 (a) is Escherichia coli plate antibacterial effect, Figure 4 (b) is the antibacterial effect of Bacillus subtilis plate. From Figure 4 It can be clearly seen that Ag / ZIF-8 has significant antibacterial effect on both Gram-positive bacteria and Gram-negative bacteria, and the antibacterial effect is more obvious with the increase of Ag / ZIF-8 concentration. It can be seen that Ag / ZIF-8 has broad-spectrum antibacterial activity.

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Abstract

The invention relates to an Ag / ZIF-8 antibacterial agent and a preparation method thereof, and belongs to the technical field of nano materials. The invention provides the Ag / ZIF-8 composite anti-bacterial agent and the preparation method. The Ag / ZIF-8 composite anti-bacterial agent is prepared by dispersing Ag nanoparticles onto ZIF-8 by impregnation and reduction. The used ZIF-8 material has a multi-stage pore structure and a large specific surface area, and has good stabilization and dispersion effects on Ag nanoparticles. According to the Ag / ZIF-8 anti-bacterial agent, the provided Ag / ZIF-8 nano anti-bacterial agent has small particles and a spherical or polyhedral structure with the particle diameter of 10-200nm, thereby being beneficial to increasing the adhesion of the Ag / ZIF-8 anti-bacterial agent to bacteria; the Ag nanoparticles and the ZIF-8 have a synergetic anti-bacterial effect, multiple indicating bacteria present efficient and board spectrum anti-bacterial effects; thepreparation method is simple in process, economical and feasible.

Description

technical field [0001] The invention belongs to the technical field of nanometer materials, and in particular relates to an Ag / ZIF-8 nanometer antibacterial agent and a preparation method thereof. Background technique [0002] Infectious diseases caused by pathogenic bacteria are an important public health problem. With the overuse of antibiotics and chemical antiseptics, drug-resistant bacteria are becoming more common, which greatly increases healthcare costs and mortality. The development of new and effective antimicrobial materials is the key to control the growth of pathogenic bacteria. As a bacteriostatic agent, metallic silver has the advantages of broad antibacterial spectrum, not easy to make bacteria resistant and no toxic side effects, and has been used to control infection for a long time. However, the traditional silver-based bacteriostatic agents have the disadvantages of too fast release of silver ions and poor antibacterial persistence. With the rapid devel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/38A61K9/14A61K47/22A61P31/04C08G83/00A61K31/787
Inventor 张艳梅张欣张祥金黎明权春善
Owner DALIAN NATIONALITIES UNIVERSITY
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