Skin repair preparation and preparation method and application thereof

A preparation and skin surface technology, applied in the fields of medical science, bandages, absorbent pads, etc., can solve the problems of limited clinical use and cumbersome preparation, and achieve the effect of promoting epithelial cell growth, accelerating wound healing, and promoting wound healing.

Active Publication Date: 2019-05-17
北京方诣生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention solves the problems in the prior art that the pr...

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  • Skin repair preparation and preparation method and application thereof
  • Skin repair preparation and preparation method and application thereof
  • Skin repair preparation and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0072] (1) Dissolve 50 g of medical grade pullulan with a molecular weight of 200 KDa, 200 g of succinic anhydride, and 10 g of 4-dimethylaminopyridine in 2500 mL of dimethyl sulfoxide, react at 50°C for 24 hours, and dialyze the reaction solution for 48 hours. The dialysate was pre-frozen at -60°C for 12h, and the obtained pre-frozen product was freeze-dried at -60°C and a vacuum of 0.1Pa for 24h to obtain succinyl pullulan;

[0073] (2) Weigh 50 g of succinyl pullulan prepared in step (1), 10 g of 4-dimethylaminopyridine, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride Dissolve 25 g in 3000 mL of dimethyl sulfoxide and stir magnetically at room temperature to obtain a succinyl pullulan solution;

[0074] (3) Dissolve 50 g of medical grade hyaluronic acid with a molecular weight of 5KDa in 2500 mL of dimethyl sulfoxide, stir evenly, and obtain a hyaluronic acid solution;

[0075] (4) 2500 mL of the hyaluronic acid solution prepared in step (3) was added dropwise ...

Embodiment 2

[0080] (1) Weigh 50g of medical grade chitosan with a molecular weight of 50KDa and 150g of succinic anhydride dispersed in methanol solution, react at 50°C for 24h, dialyze the reaction solution for 48h, and pre-freeze the dialysate at -60°C for 12h. The frozen product was freeze-dried at -60°C and 0.1Pa vacuum for 24h to obtain succinyl chitosan;

[0081](2) Weigh 50 g of succinyl chitosan prepared in step (1), 10 g of 4-dimethylaminopyridine, 25 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride Dissolve in 2500 mL of dimethyl sulfoxide and stir magnetically at room temperature to obtain a succinyl chitosan solution;

[0082] (3) Weigh 25 g of medical grade pullulan with a molecular weight of 200KDa and dissolve it in 200 mL of dimethyl sulfoxide, stir evenly to obtain a pullulan solution;

[0083] (4) Dissolve 25 g of medical grade hyaluronic acid with a molecular weight of 5KDa in 200 mL of dimethyl sulfoxide, stir evenly to obtain a hyaluronic acid solutio...

Embodiment 3

[0086] (1) Dissolve 50 g of medical grade pullulan with a molecular weight of 200 KDa, 50 g of succinic anhydride, and 5 g of 4-dimethylaminopyridine in 2500 mL of dimethyl sulfoxide, react at 60°C for 36 hours, and dialyze the reaction solution for 48 hours. The dialysate was pre-frozen at -60°C for 12h, and the obtained pre-frozen product was freeze-dried at -60°C and a vacuum of 0.1Pa for 24h to obtain succinyl pullulan;

[0087] (2) Weigh 50 g of succinyl pullulan prepared in step (1), 5 g of 4-dimethylaminopyridine, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride 50 g was dissolved in 3000 mL of dimethyl sulfoxide, and magnetically stirred at room temperature to obtain a succinyl pullulan solution;

[0088] (3) Weigh 50 g of medical grade hyaluronic acid with a molecular weight of 5KDa and dissolve it in 2000 mL of dimethyl sulfoxide, stir evenly to obtain a hyaluronic acid solution;

[0089] (4) 2000 mL of the hyaluronic acid solution prepared in step (3) wa...

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Abstract

The invention discloses a skin surface wound repair preparation and a preparation method and application thereof. Natural high-molecular polysaccharides such as chitosan, hyaluronic acid, pullulan andthe like are used as raw materials, succinic anhydride is used as a connecting arm, 4-dimethylaminopyridine is used as a catalyst, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is takenas a dehydrating agent, a product is dialyzed to remove a catalyst and the dehydrating agent, freeze-drying is executed to obtain hyaluronic acid-based pullulan, and the hyaluronic acid-based pullulan is physically mixed with chitosan to prepare a membrane, and a skin wound repair membrane preparation is obtained; or hyaluronic acid and pullulan are linked by adopting succinylated chitosan to obtain the skin surface wound repair preparation prepared from the chemically grafted chitosan-hyaluronic acid-pullulan high molecular material. The skin surface wound repair preparation has good biocompatibility, can effectively stop bleeding, resist bacteria, absorb wound area liquid, keep wounds moist, promote epithelial cell growth and accelerate wound healing, and has the advantages of wide rawmaterial source, low cost, mild preparation conditions, simple production route and easy control of product quality.

Description

technical field [0001] The invention relates to the field of medical materials, in particular to a skin repair preparation and a preparation method and application thereof. Background technique [0002] The skin is an essential interface between the body and the environment, and the key factors in death from skin trauma are primarily uncontrolled bleeding and infection of the wound. Conventional wound dressings in clinical applications are natural or synthetic bandages, such as cotton balls, gauze, etc., which have poor hemostatic effect, cannot prevent infection, nor promote wound healing, and even adhere to the dry wound surface to cause secondary trauma. Many clinical hemostatic preparations use hemostatically active proteins such as fibrin, collagen and thrombin, which are difficult to store and use outside the hospital, although their hemostatic effect is remarkable. For wound infection, the main reason is bacterial infection caused by Staphylococcus aureus, Escherichi...

Claims

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Application Information

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IPC IPC(8): A61L15/28A61L15/42A61L15/44A61L15/46
Inventor 杨文智付国亮李凯悦李海鹰
Owner 北京方诣生物医药有限公司
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