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Kit for detecting foot and mouth disease virus 3AB antibody, and detection method thereof

A foot-and-mouth disease virus and kit technology, applied in the field of enzyme-linked immunization, can solve problems such as hindering the development of aquaculture, unsuitable for grass-roots detection, economic losses of farmers, etc., achieving good sensitivity, specificity and repeatability, simple operation and low cost. Effect

Inactive Publication Date: 2019-05-17
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Foot-and-mouth disease virus is easy to spread through the air, highly contagious, and spreads quickly. Susceptible animals will also die when their resistance is weak, causing huge economic losses to farmers and seriously hindering the development of the breeding industry.
[0003] At present, the diagnostic method of FMD virus 3AB antibody and the evaluation method of vaccine immunity can only be completed in the laboratory, which is not suitable for grassroots detection. It is necessary to establish a more sensitive, fast and simple visual detection method

Method used

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  • Kit for detecting foot and mouth disease virus 3AB antibody, and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] 1. Preparation of sample diluent, washing solution and stop solution

[0052] Coating solution is 0.05M carbonate buffer: Na 2 CO 3 1.59g, NaHCO 3 2.93g, add distilled water to make up to 1000mL, pH is 9.6;

[0053] The sample diluent is a washing solution containing 50g / L skimmed milk powder;

[0054] The washing solution is 0.01M pH 7.2 phosphate buffer containing 0.05% Tween-20, and its formula is:

[0055] NaCl: 8.5g, NaH 2 PO 4 2H 2 O: 0.356g, NaH 2 PO 4 12H 2 O: 2.772g, after mixing it, dissolve it with distilled water and set the volume to 1000mL, then add 0.5mL Tween-20 to it to obtain the above washing solution;

[0056] The stop solution is: configure 1% SDS solution.

[0057] 2. Determination of the optimum coating concentration of antigen and the optimum dilution of serum

[0058] The results showed that when the dilution of the antigen was 2.0 μg / mL per well, the serum was diluted 1:40 times, and the enzyme-labeled secondary antibody was diluted...

Embodiment 2

[0064] Specificity test of the kit:

[0065] Detect known swine influenza virus, classical swine fever virus, Japanese encephalitis virus, porcine circovirus, porcine parvovirus, pseudorabies virus, PRRS virus and foot-and-mouth disease virus 3AB positive serum samples respectively by the method that embodiment 1 establishes, negative It is colorless, positive is blue. see results figure 1 .

[0066] according to figure 1 It can be seen that the kit provided by the invention can accurately detect foot-and-mouth disease, has no cross-reaction with other viruses, and has specificity.

[0067] As can be drawn from the above examples, the kit provided by the invention detects the antibody of foot-and-mouth disease virus 3AB, which has the advantages of high efficiency, sensitivity, specificity and good repeatability. The method is easy to operate, fast, and low in cost, and can be visualized at room temperature The detection is suitable for promotion in clinical application, a...

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Abstract

The invention provides a kit for detecting foot and mouth disease virus 3AB antibody, and a detection method thereof, and relates to the technical field of the enzyme linked immunosorbent assay (ELISA). The kit comprises an elisa plate and DON-HRP-Conjugated; the elisa plate is coated by the foot and disease virus 3AB protein. The kit provided by the invention detects the antibody of the foot andmouth disease virus 3AB, and has the advantages of being efficient, sensitive in specificity and good in repeatability. The method is simple and rapid in operation and low in cost, and the visual detection can be performed under room temperature; the kit and the detection method are suitable for popularizing in the clinical application, and a reliable technical measure is provided for the quick detection of the foot and mouth disease virus 3AB antibody.

Description

technical field [0001] The invention relates to the technical field of ELISA, in particular to a kit for detecting foot-and-mouth disease virus 3AB antibody and a detection method thereof. Background technique [0002] Foot-and-mouth disease is an acute, febrile and highly contagious infectious disease caused by foot-and-mouth disease virus (FMDV), which mainly affects artiodactyls and often spreads among sheep, cattle, pigs and other animals. During the onset, blisters appeared on the lips and hooves of susceptible animals, accompanied by symptoms such as fever and loss of appetite, and the body weight and milk production of affected animals dropped significantly. Foot-and-mouth disease virus is easy to spread through the air, highly contagious, and spreads rapidly. The susceptible animals will also die when their resistance is weak, causing huge economic losses to farmers and seriously hindering the development of aquaculture. [0003] At present, the diagnostic method of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/535G01N33/58G01N33/68G01N21/78
Inventor 陈豪泰张永光孙跃峰祁林林张杰潘丽
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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