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A high-throughput electrochemical sensor for microdroplets based on micropillar arrays

A microcolumn array, electrochemical technology, applied in the direction of material electrochemical variables, scientific instruments, material analysis through electromagnetic means, etc., can solve the problems of difficult detection implementation, lack of technical devices, etc., to solve the problem of large liquid volume and wide application foreground effect

Active Publication Date: 2020-09-18
UNIV OF SCI & TECH BEIJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention provides a micro-droplet high-throughput electrochemical sensor based on micro-column arrays for the problems of difficult implementation of micro-solution electrochemical detection and lack of technical devices for high-throughput electrochemical detection.

Method used

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  • A high-throughput electrochemical sensor for microdroplets based on micropillar arrays
  • A high-throughput electrochemical sensor for microdroplets based on micropillar arrays
  • A high-throughput electrochemical sensor for microdroplets based on micropillar arrays

Examples

Experimental program
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Effect test

preparation example Construction

[0033] Step 1. Preparation of the microcolumn platform: the present invention takes the 3D printing polylactic acid template as an example, uses 3D printing technology to print out a polylactic acid template with a diameter of 1-4mm and a thickness of 1.5mm, cleans it with ethanol, and uses adhesive tape Seal one side of the template and paste it on the petri dish, mix PDMS with curing agent and vacuumize to remove air bubbles, then pour it into the petri dish, and after curing in an oven at 50-60°C for 4-5 hours, carefully peel off the template to obtain a microcolumn array platform.

[0034] Step 2. Electrode wire integration: Soak the microcolumn platform obtained in step 1 and the purchased gold, silver and platinum electrode wires in ethanol for 10-15 minutes, take them out and dry them, and soak the silver wires in saturated sodium chloride After 1-2 hours in the solution, take it out as a reference electrode. Three kinds of electrodes are inserted into the microcolumn ...

Embodiment 1

[0038] A method for preparing a micro-droplet high-throughput electrochemical sensor based on a micro-column array, comprising:

[0039] Step 1. Preparation of microcolumn platform: The present invention takes 3D printing polylactic acid template as an example, designs a model with a cylindrical groove array through 3D Max, prints out the designed polylactic acid model by using 3D printing technology, and uses the model to Soak in ethanol and ultrasonic for 5-10 minutes to clean thoroughly, seal one side of the model with tape so that PDMS can only enter the hollow cylinder from one side, and then fix the template on the Petri dish with double-sided adhesive to avoid floating. Mix PDMS and curing agent evenly at a ratio of 15:1, place it in a vacuum oven at 60°C for 10-15 minutes to remove air bubbles in the mixture, and pour it evenly on the polylactic acid model, and let it stand for 4-5 minutes to remove the bubbles generated during pouring. bubbles, and then placed in a 60...

Embodiment 2

[0043] Analysis of micro-droplet carrying capacity of micro-column platform, including:

[0044] Step 1. The influence of the degree of inclination of the microcolumn on the fixation of the droplet: the droplet is added to the surface of the microcolumn, and the droplet will not fall off when the microcolumn platform is tilted to 45°, 90° and upside down, indicating that the microcolumn Droplets can be well fixed for detection.

[0045] Step 2. Add the droplet on the surface of the microcolumn, and moderately stretch, twist and bend the microcolumn platform, and the droplet can be stably maintained on the surface of the microcolumn, which shows that the microcolumn platform has good flexibility and Very well fixed droplets for detection.

[0046] Step 3. Analysis of the carrying capacity of the microcolumns on different types of solutions: water, PBS, serum, milk, coffee, dyes, sweat, 1M hydrochloric acid and 1M sodium hydroxide and other solutions were added dropwise to the ...

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Abstract

The invention provides a micro-droplet high-throughput electrochemical sensor based on a microcolumn array, and belongs to the technical field of electrochemical sensors. The sensor comprises a droplet fixing unit and an electric signal transmission unit, and the droplet fixing unit can fix micro-droplets on the surfaces of microcolumns; the electric signal transmission unit is composed of a three-electrode system and an integrated circuit board in each microcolumn. According to the sensor, a micro-droplet array platform is combined with an electrochemical technology, and thus the sensor has the advantages of high sensitivity and accuracy of electrochemical detection, also solves the problems that traditional electrochemical detection liquid is large in volume, and high-throughput detection is hard to achieve, and has a wide application prospect in the fields such as disease multi-marker detection, drug screening and high-throughput cell analysis.

Description

technical field [0001] The invention relates to the technical field of electrochemical sensors, in particular to a micro-droplet high-throughput electrochemical sensor based on a micro-column array. Background technique [0002] With the development of science and technology, traditional biochemical research tends to be miniaturized and simplified. Nowadays, the relatively mature method is to separate the micro-droplets through a porous plate, and use the characteristics of small droplet size, simple manipulation, each droplet can be an independent unit and easy automation to realize protein crystallization, gene recombination and DNA analysis, etc. Experiments have achieved simple and cheap high-throughput micro-droplet manipulation. However, this porous plate only has the ability to carry micro-droplets and does not have chemical sensing properties, which greatly limits its application in the analysis of body fluid disease markers, drug screening, and high-throughput cell...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/48G01N27/327
Inventor 许太林宋永超张学记
Owner UNIV OF SCI & TECH BEIJING
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