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Lincomycin rapid time resolution fluorescent immunity chromatography quantitative detection test strip

A time-resolved fluorescence, lincomycin technology, applied in analytical materials, measuring devices, instruments, etc., can solve the problems of low sensitivity, detection influence, large differences between batches, etc., and achieve the effect of high sensitivity

Inactive Publication Date: 2019-05-28
ZHENJIANG YITE BIOTECH DEV CO LTD
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

The rapid detection products developed based on colloidal gold labeling technology have problems such as qualitative or semi-quantitative, and large batch-to-batch differences; although the batch-to-batch difference of colored latex microspheres has improved, the sensitivity is still low and can only be qualitative or semi-quantitative ; The sensitivity of immunochromatography based on fluorescein labeling technology has been greatly improved, and quantitative detection can also be performed, but because the sample contains a high fluorescent background signal and the stock shift is small, it will have a greater impact on the detection

Method used

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  • Lincomycin rapid time resolution fluorescent immunity chromatography quantitative detection test strip
  • Lincomycin rapid time resolution fluorescent immunity chromatography quantitative detection test strip
  • Lincomycin rapid time resolution fluorescent immunity chromatography quantitative detection test strip

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0035] 1. Preparation of Components of Test Strips

[0036] 1.1 Preparation of quality control microspheres Preparation of quality control microspheres coated with biotinylated γ-globulin (BGG)

[0037] ⑴ Preparation of biotinylated BGG

[0038] with 0.1 M NaCNBH 3 BGG (purchased from Pel-Freez Biological) was prepared into a 10 mg / mL solution, and Biotin-X-X-NHS (N-hydroxysuccinimide modified biotin, manufacturer: SIGMA, Product number: B3295) solution to 16.2 mg / mL, add Biotin-X-X-NHS solution to BGG solution according to the amount of 1 mg BBG protein and 5.4 μl Biotin-X-X-NHS solution, mix well and place overnight at 4°C. Dialysis was used to remove free unreacted biotin, and the dialysate was biotin-labeled protein dialysate buffer (0.1 M Tris, 0.3 M Nacl, 0.005 MEDTA-Na-2H 2 0, pH8.0). After dialysis, the protein concentration was determined by BCA method.

[0039] ⑵ Use the above-mentioned labeled protein to coat the aldehyde-modified fluorescent microspheres

[0...

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Abstract

The invention relates to a preparation method and application of a lincomycin rapid time resolution fluorescent immunity chromatography quantitative detection test strip. The test strip is composed ofa Fusion 5 membrane, nitrocellulose and absorbent paper. On the basis of the competition law, time resolution fluorescent microballoon spheres are adopted as an immune marker, and accurate and rapidquantitative detection is performed on a to-be-detected antigen.

Description

technical field [0001] The invention belongs to the field of food safety detection, and in particular relates to a detection method for harmful residues in food, in particular to a time-resolved fluorescent immunochromatographic test strip for quantitative detection of lincomycin, a preparation method and application thereof. Background technique [0002] Lincomycin is a broad-spectrum antibiotic, commonly used in the treatment of various infectious diseases in animals, and has a strong inhibitory effect on a variety of pathogenic bacteria. Lincomycin has serious side effects, which can inhibit the hematopoietic function of human bone marrow and cause human diseases such as aplastic anemia. Therefore, lincomycin residues in animal foods pose a huge threat to human health. [0003] At present, the determination methods of lincomycin and its metabolites in food of animal origin are mainly physical and chemical detection methods. For example, chemical analysis is used to determ...

Claims

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Application Information

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IPC IPC(8): G01N33/533G01N33/558G01N33/577
Inventor 洪霞杜霞袁超
Owner ZHENJIANG YITE BIOTECH DEV CO LTD
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