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Total bilirubin measuring kit

A technology of total bilirubin and kits, which can be used in biological testing, material inspection products, etc., and can solve problems such as false positives

Active Publication Date: 2019-05-28
HEBEI INST FOR DRUG & MEDICAL DEVICE CONTROL (HEBEI INST FOR COSMETICS CONTROL) +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the vanadate method and the nitrite method are interfered by interstitial gallbladders, such as free bilirubin, delta-bilirubin, etc., and are prone to false positives in clinical practice.

Method used

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Experimental program
Comparison scheme
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Embodiment

[0042]

[0043]

[0044] This test detects the method for total bilirubin assay kit, comprises the steps:

[0045] 1) The instrument used in the experiment is Hitachi 7600 automatic biochemical analyzer (sensitivity 0.001 and above, and the temperature range of its constant temperature device is within ±0.1 ℃), and the water shall not be lower than GB / T 6682- In 2008, the requirements for third-class water.

[0046] 2) Edit experimental parameters: Reaction method: two-point endpoint method; dominant wavelength: 450nm; reaction temperature: 37°C; sample volume: 8.0μl; reagent R1: 240μl;

[0047] 3) Load the total bilirubin assay kit optimized according to the above standards into the Hitachi 7600 Biochemical Analyzer.

[0048] 4) Calibrate with calibration serum.

[0049] 5) Detect the quality control serum to determine the accuracy of the detection.

[0050] 6) Take the clinical blood sample and centrifuge it, separate the serum and test the result with a total bilir...

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Abstract

The invention discloses a total bilirubin measuring kit. The total bilirubin measuring kit is a liquid composed of a reagent R1 and a reagent R2; citric acid is selected and can well ensure the pH value of a whole specimen preprocessing system; EDTA-Na2 is used for eliminating interference caused by divalent metal ions in a measurement process; four surfactants, namely lauryl betaine, polyethyleneglycol monooleyl ether, hexadecyl trimethyl ammonium bromide and tetradecyl trimethyl ammonium bromide are added, and hydrazine dihydrochloride is added into the reagents to further eliminate interference caused by non-bilirubin substances, so that false positives of measurement data are avoided; sodium nitrite is added to directly oxidize bilirubin into biliverdin, but the selected amount of sodium nitrite is quite small so that it can be ensured that in a whole reaction process, not only can bilirubin be completely converted, but also little residual sodium nitrite exists to avoid reactionbetween surplus sodium nitrite and the other substances; sodium chloride is added to adjust the ion concentration and control reaction speed; added Porlin 300 serves as a biological preservative and not only cannot form interference with the reaction substances but also can prevent bacteria from converting nitrite into nitrate.

Description

technical field [0001] The invention relates to a kit for measuring total bilirubin. Background technique [0002] Total bilirubin is the general term for conjugated and unconjugated bilirubin produced by heme metabolism in the human body. It is mainly used to diagnose whether there is liver disease or whether there is abnormality in the biliary tract. The reason for the low total bilirubin may be because Iron deficiency anemia. Therefore, the determination of total bilirubin in human serum, plasma and urine is one of the most routine items in medical testing. [0003] Determination of total bilirubin is an important test item in liver and gallbladder function tests. At present, the methods for measuring serum bilirubin mainly include diazo method and enzymatic method. The diazo method reagent is unstable and must be temporarily prepared by sodium nitrite and sulfamic acid, and the storage time is short; the second is that the specificity of the diazo reagent reacting wit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/72
Inventor 李挥王丽杨光刘若锦邵文亮邹志良庞刚吴松波
Owner HEBEI INST FOR DRUG & MEDICAL DEVICE CONTROL (HEBEI INST FOR COSMETICS CONTROL)
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