Desalting and decoloring method for cistanche total oligosaccharide

Abstract

The invention discloses a desalting and decoloring method for cistanche total oligosaccharide. The method is characterized in that an ion exchange macroporous adsorption resin column is used for desalting and decoloring the cistanche total oligosaccharide part, wherein the cistanche total oligosaccharide part is an active extract containing cistanche total oligosaccharides after extracting, separating and purifying of cistanche medicinal materials, the ion exchange macroporous adsorption resin column comprises a cation exchange macroporous adsorption resin column and an anion exchange macroporous adsorption resin column which are connected in series. According to the invention, macroporous resin has the advantages of strong adsorption capacity, easy elution, high mechanical strength and the like, and the macroporous resin and the solution are subjected to anion-cation exchange to remove salts and pigments in the solution, so that the desalting and decolorizing capacity under dynamic adsorption elution is high, and the obtained cistanche total oligosaccharide has good application prospect and market value.

Description

technical field

[0001] The invention relates to a method for separating oligosaccharides from Cistanche deserticola, in particular to a method for desalting and decolorizing total oligosaccharides from Cistanche deserticola by using resin. Background technique

[0002] Cistanche is the dried succulent stem with scaly leaves of Cistanche deserticola Y.C.Ma or Cistanche tubulosa (Schenk) R.Wight, a plant of the family Danaceae. It is often used for symptoms such as deficiency of kidney yang, deficiency of essence and blood, impotence and infertility, weakness of waist and knees, weakness of muscles and bones, dryness of the intestine and constipation. With the aging of the Chinese population and the increasing pressure of modern life, the prevalence of constipation is gradually increasing. As a traditional laxative in China, Cistanche has also been paid more and more attention. Previous studies have shown that the main pharmacodynamic substance of Cistanche's laxative effect ...

Examples

Embodiment 1

[0041] The screening test of embodiment 1 resin

[0042] 1) Extraction: crush Cistanche deserticola, add 10 times the weight of pure water, heat and reflux for extraction 3 times, each time for 1.0 h, and combine the filtrates.

[0043] 2) Concentration: Concentrate under reduced pressure until the relative density reaches 1.15 (measured at 60°C), add 95% (v / v) ethanol solution to elute statically until the alcohol content reaches 50%, stand overnight, filter, and concentrate the filtrate under reduced pressure to 1.15 (measured at 50°C).

[0044] 3) Pretreatment of macroporous resin: Soak AB-8 macroporous resin with 95% (v / v) ethanol solution for 12 hours, first rinse with 95% (v / v) ethanol until the ethanol flowing out is free of turbidity, and then Wash with distilled water until there is no alcohol smell; then soak in 4% (v / v) HCl solution for 4 hours, wash with distilled water until neutral, then soak in 4% (v / v) NaOH solution for 4 hours, wash with distilled water until...

Embodiment 2

[0057] Example 2 Ionic resin desalination and decolorization dynamic adsorption test of Cistanche total oligosaccharides

[0058] Pack LS-840 acidic cation exchange resin and LS-850 basic anion exchange resin into columns according to the volume ratio of 2:1, 1.5:1, 1.2:1, 1:1, 0.8:1 respectively, according to LS-840 resin LS Sequential tandem of -850 resins. Take 100ml of Cistanche oligosaccharides prepared by the method in Example 1 (diluted with pure water to a concentration of 15%) and put it on the column, control the flow rate at 2BV / h, wait until the sugar content of the effluent is greater than 0, collect the eluate, and calculate the desalination rate and decolorization rate.

[0059] Depend on figure 1 It can be seen that the desalination effect is the best when the ratio of anion to cation resin is 1.2:1, and it also shows that the salts in the total oligosaccharides of Cistanche deserticola mainly exist in the form of weakly basic anions. The resin ratio has lit...

technical field

[0001] The invention relates to a method for separating oligosaccharides from Cistanche deserticola, in particular to a method for desalting and decolorizing total oligosaccharides from Cistanche deserticola by using resin. Background technique

[0002] Cistanche is the dried succulent stem with scaly leaves of Cistanche deserticola Y.C.Ma or Cistanche tubulosa (Schenk) R.Wight, a plant of the family Danaceae. It is often used for symptoms such as deficiency of kidney yang, deficiency of essence and blood, impotence and infertility, weakness of waist and knees, weakness of muscles and bones, dryness of the intestine and constipation. With the aging of the Chinese population and the increasing pressure of modern life, the prevalence of constipation is gradually increasing. As a traditional laxative in China, Cistanche has also been paid more and more attention. Previous studies have shown that the main pharmacodynamic substance of Cistanche's laxative effect ...

Examples

Embodiment 1

[0041] The screening test of embodiment 1 resin

[0042] 1) Extraction: crush Cistanche deserticola, add 10 times the weight of pure water, heat and reflux for extraction 3 times, each time for 1.0 h, and combine the filtrates.

[0043] 2) Concentration: Concentrate under reduced pressure until the relative density reaches 1.15 (measured at 60°C), add 95% (v / v) ethanol solution to elute statically until the alcohol content reaches 50%, stand overnight, filter, and concentrate the filtrate under reduced pressure to 1.15 (measured at 50°C).

[0044] 3) Pretreatment of macroporous resin: Soak AB-8 macroporous resin with 95% (v / v) ethanol solution for 12 hours, first rinse with 95% (v / v) ethanol until the ethanol flowing out is free of turbidity, and then Wash with distilled water until there is no alcohol smell; then soak in 4% (v / v) HCl solution for 4 hours, wash with distilled water until neutral, then soak in 4% (v / v) NaOH solution for 4 hours, wash with distilled water until...

Embodiment 2

[0057] Example 2 Ionic resin desalination and decolorization dynamic adsorption test of Cistanche total oligosaccharides

[0058] Pack LS-840 acidic cation exchange resin and LS-850 basic anion exchange resin into columns according to the volume ratio of 2:1, 1.5:1, 1.2:1, 1:1, 0.8:1 respectively, according to LS-840 resin LS Sequential tandem of -850 resins. Take 100ml of Cistanche oligosaccharides prepared by the method in Example 1 (diluted with pure water to a concentration of 15%) and put it on the column, control the flow rate at 2BV / h, wait until the sugar content of the effluent is greater than 0, collect the eluate, and calculate the desalination rate and decolorization rate.

[0059] Depend on figure 1 It can be seen that the desalination effect is the best when the ratio of anion to cation resin is 1.2:1, and it also shows that the salts in the total oligosaccharides of Cistanche deserticola mainly exist in the form of weakly basic anions. The resin ratio has lit...

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