A multi-target magnetic immunochemiluminescence microfluidic chip detection platform and method
A microfluidic chip, chemiluminescence technology, applied in chemical instruments and methods, chemiluminescence/bioluminescence, analysis by chemical reaction of materials, etc., can solve the problem of excessive reagent consumption, insufficient mixing, and simultaneous detection of multiple targets Restricted and other problems, to achieve the effect of convenient operation, fast detection and low cost
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Embodiment 1
[0032] The chemiluminescent molecule in this example is HRP enzyme, and the luminescent system is luminol chemiluminescent system. See the attached schematic diagram for details. image 3 ;
[0033] (1) if image 3 As shown, there are three target molecules in the sample. When the sample is injected through the sample hole, it flows through the liquid flow connection channel to reconstitute the antibody in the coating area; the first target reacts with the corresponding magnetic-labeled antibody and enzyme-labeled antibody through the mixing channel. , forming the first target sandwich complex; the particle size of the magnetic beads is 10-1000nm, preferably 500nm;
[0034] (2) When the first target sandwich complex flows through the capture area, due to the magnetic force at the position of the capture area, the first target sandwich complex is captured and fixed in this area,
[0035] (3) The remaining samples continue to flow through the next antigen immobilization area, ...
Embodiment 2
[0041] A multi-target magnetic immunochemiluminescence microfluidic chip detection platform, its schematic diagram is as follows figure 1 As mentioned above, the detection platform includes a sampling hole 1, a liquid flow connection channel 2, a target detection area 3, a waste liquid port 4, a cleaning liquid pool 5, and a luminescent liquid pool 6; there are at least two target detection areas 3; The target detection area 3 includes a sequentially connected coating area 7, a mixing area 8 and a capture area 9; the liquid flow connection channel 2 is used to transport the sample injected in the sample hole 1 to the target detection area 3; different The coating area 7 in the target detection area 3 contains antibody A and antibody B that can specifically bind to different targets in the sample at the same time, and the antibody A is a chemiluminescence molecule or an enzyme-labeled antibody that can make the substrate emit light , the antibody B is a magnetic bead-labeled an...
Embodiment 3
[0043] A multi-target magnetic immunochemiluminescence microfluidic chip detection platform, its schematic diagram is as follows figure 2 As mentioned above, the detection platform includes a sampling hole 1, a liquid flow connection channel 2, a target detection area 3, a waste liquid port 4, a cleaning liquid pool 5, and a luminescent liquid pool 6; there are at least two target detection areas 3; The target detection area 3 includes a sequentially connected coating area 7, a mixing area 8 and a capture area 9; the liquid flow connection channel 2 is used to transport the sample injected in the sample hole 1 to the target detection area 3; different The coating area 7 in the target detection area 3 contains antibody A and antibody B that can specifically bind to different targets in the sample at the same time, and the antibody A is a chemiluminescence molecule or an enzyme-labeled antibody that can make the substrate emit light , the antibody B is a magnetic bead-labeled a...
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