Method for inducing pumpkin haplobiont and culture medium using induction method

Abstract

The invention discloses a method for inducing a pumpkin haplobiont. The method comprises the following steps that surface disinfection is carried out on an unfertilized ovary of a pumpkin, the parts,without ovule, at two longitudinal ends of the ovary and skin of the ovary are cut off and then the remaining ovary is transversely cut into ovary slices with the thickness of 1-2 mm, and the ovary slices are inoculated into an induction culture medium for induction culture after being sterilized; when an obtained embryo has an obvious morphological upper end and a morphological lower end, the embryo is transplanted into a seedling culture medium for seedling culture until a regenerated plant is obtained; the pumpkin haplobiont is screened out through ploidy identification. The method is easyto operate, the haplobiont can be obtained within 60 days, the embryo yield and the plant regeneration rate are relatively high, the breeding period is shortened, the breeding efficiency is greatly improved, and the haploid rate is high. The invention also provides the induction culture medium. Only one hormone is added on the basis of an MS culture medium, preparation is convenient, the price islow, and the induction culture medium can be effectively applied to unfertilized ovary isolated culture of the pumpkin.

Description

technical field [0001] The invention belongs to the technical field of in vitro regeneration of plants, and in particular relates to a method for inducing pumpkin haploid plants and a culture medium used in the inducing method. Background technique [0002] Pumpkin (Cucurbita spp.) is resistant to barrenness, easy to cultivate, strong adaptability and stress resistance, high yield, and good economic benefits. Today, my country's pumpkin sowing area and output rank first in the world. my country has a vast territory and rich resources, and has abundant pumpkin germplasm resources. Since the 1950s, three major surveys and collections of crop variety resources have been carried out across the country, and about 1,100 pumpkin germplasm resources have been obtained, but pumpkin resources are innovative. The foundation of breeding research is very weak, the breeding technology is relatively backward, and there is a serious shortage of high-quality and special-purpose varieties with...

AI Technical Summary

Problems solved by technology

[0004] Deng Ying (2017) disclosed a medium for in vitro culture of pumpkin unfertilized ovaries to induce gynogenesis and its application. The invention successfully obtained regenerated plants after three months of induction by adding a certain concentration of TDZ to the medium. However, there are problems such as low embryonic rate, small number of regenerated plants and unidentified ploidy.

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