Extracting method for lincomycin hydrochloride

A technology of lincomycin hydrochloride and lincomycin hydrochloride, applied in the field of lincomycin hydrochloride preparation, can solve the problems of long production cycle, low filtration precision, affecting resin adsorption efficiency and processing capacity, etc., to avoid thermal degradation, The effect of improving product quality

Active Publication Date: 2019-06-28
HEC PHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this process uses plate and frame filter press for front-end solid-liquid separation, and the filtration accuracy is low. The sample liquid contains some suspended solids and macromolecular impurities such as proteins and polypeptides, which affects the adsorp

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  • Extracting method for lincomycin hydrochloride

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A kind of extracting method of lincomycin hydrochloride, described method comprises the following steps:

[0035] 1.1 Fermentation broth microfiltration

[0036] Transfer 400Kg of fermented liquid into the intermediate tank, use 30% NaOH solution to adjust the pH to 7.1, and directly use ceramic membrane for microfiltration. When the filtrate obtained by filtering is 200Kg, continuously add cleaning water to the concentrated end of the fermented liquid for dialysis, and when the quality of the filtrate reaches 900Kg, stop the microfiltration (selecting a ceramic membrane with a membrane aperture of 500nm).

[0037] 1.2 Concentration of microfiltrate by nanofiltration

[0038] Mix the obtained filtrate evenly, perform nanofiltration, and concentrate to 1 / 6 of the volume of the microfiltrate to obtain 150Kg of the aqueous phase concentrate.

[0039] 1.3 Aqueous phase concentrated liquid alkalization treatment and filtration

[0040] Transfer the aqueous phase concentrate...

Embodiment 2

[0052] 2.1 Microfiltration of fermentation broth

[0053] Transfer 400Kg of fermented liquid into the intermediate tank, use 10% NaOH solution to adjust the pH to 7.4, and directly use the ceramic membrane for micro-processing. When the filtrate obtained by filtration is 200Kg, add cleaning water to the fermented broth concentration end continuously for dialysis, and when the filtrate quality reaches 900Kg, stop the microfiltration (select the 1200nm ceramic membrane with membrane aperture).

[0054] 2.2 Concentration of microfiltrate by nanofiltration

[0055] Mix the obtained filtrate evenly, perform nanofiltration, and concentrate to 1 / 6 of the volume of the microfiltrate to obtain 150Kg of the aqueous phase concentrate.

[0056] 2.3 Aqueous phase concentrated liquid alkalization treatment and filtration

[0057] Transfer the aqueous phase concentrate to the intermediate tank, start stirring, use 30% NaOH solution to adjust the pH to 11.0-11.5, add 5Kg of polyaluminum chl...

Embodiment 3

[0068] 3.1 Microfiltration of fermentation broth

[0069] Transfer 500Kg of fermented liquid into the intermediate tank, without adjusting the pH of the fermented liquid, and directly use the ceramic membrane for microfiltration. When the filtrate obtained by filtering is 200Kg, add cleaning water to the fermented broth concentration end continuously for dialysis, and when the filtrate quality reaches 1100Kg, stop the microfiltration (select the ceramic membrane with a membrane aperture of 500nm).

[0070] 3.2 Concentration of microfiltrate by nanofiltration

[0071] The obtained filtrate was mixed uniformly, then subjected to nanofiltration, and concentrated to 1 / 5 of the volume of the microfiltrate to obtain 220Kg of the aqueous phase concentrate.

[0072] 3.3 Aqueous phase concentrated liquid alkalization treatment and filtration

[0073] Transfer the aqueous phase concentrate to the intermediate tank, start stirring, use 30% NaOH solution to adjust the pH to 11.0-11.5, a...

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Abstract

The invention provides an extracting method for lincomycin hydrochloride. The method comprises the steps that S1, a lincomycin fermentation solution is subjected to micro-filtration and nano-filtration, and then a water phase concentration solution is obtained; S2, after the water phase concentration solution is subjected to alkalization treatment, comprehensive flocculation impurity removal is performed, a disc filter performs centrifuging, an alkalization clean solution is extracted by an extraction agent, and an extraction solution is obtained; S3, the concentration solution is concentratedand acidified to be subjected to a reaction for crystallization, and lincomycin hydrochlorid is obtained. Micro-filtration is adopted sequentially for performing solid-liquid separation and nano-filtration of the fermentation solution to obtain the water phase concentration solution, after comprehensive impurity removal, the extraction agent is adopted for extraction, and concentration, acidification and crystallization are performed to obtain lincomycin hydrochlorid. The production process is high in automatic degree, continuous and reliable, the production period is short, and meanwhile theproduct quality is improved.

Description

technical field [0001] The invention relates to the technical field of lincomycin hydrochloride preparation, in particular to a method for extracting lincomycin hydrochloride. Background technique [0002] Lincomycin (LCM for short), also known as lincomycin and lincomycin, belongs to the lincosamide antibiotics and is a spectrum antibiotic produced by Streptomyces linconinensis through aerobic fermentation. Lincomycin is mainly used clinically for various infections caused by sensitive bacteria, such as pneumonia, meningitis, endocarditis, cellulitis, tonsillitis, erysipelas and urinary system infections. Because this product has many drug routes, fewer side effects, quick control of infection, strong penetration of tissues and cells, can enter bone tissue, and has special affinity with bone, it is especially suitable for infections and diseases caused by anaerobic bacteria. Staphylococcus aureus osteomyelitis. [0003] The extraction process of lincomycin was first propo...

Claims

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Application Information

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IPC IPC(8): C07H15/16C07H1/06
Inventor 邹兵谢超余永洋
Owner HEC PHARM
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