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Research method of allelopathic mechanism of blue-green algae population competition based on surface-enhanced Raman scattering spectroscopy

A surface-enhanced Raman and allelopathic technology, applied in Raman scattering, material analysis by optical means, material excitation analysis, etc., can solve the problems of uncontrollable electromagnetic enhancement performance of biosynthetic nanoparticles and biotoxicity of nanoparticles

Active Publication Date: 2021-06-15
CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in situ surface-enhanced Raman scattering studies of intracellular molecules face problems such as the uncontrollable electromagnetic enhancement properties of biosynthesized nanoparticles, and the biotoxicity of nanoparticles during the synthesis process.

Method used

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  • Research method of allelopathic mechanism of blue-green algae population competition based on surface-enhanced Raman scattering spectroscopy
  • Research method of allelopathic mechanism of blue-green algae population competition based on surface-enhanced Raman scattering spectroscopy
  • Research method of allelopathic mechanism of blue-green algae population competition based on surface-enhanced Raman scattering spectroscopy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Preparation of a Bimetallic Crater Surface Enhanced Raman Scattering Substrate

[0033] Such as figure 1 The process flow for the preparation of the enhanced Raman scattering substrate on the surface of the bimetallic crater is shown. On the surface of hard substrates such as silicon and glass cleaned by plasma or chemical methods, the magnetron sputtering method is used to plate gold and silver films in sequence. , the thickness of the gold film is 50 nanometers, and the thickness of the silver film is 20 nanometers; wherein, in order to improve the adsorption of the gold film on the substrate surface, 2 nanometers of chromium need to be plated before the gold plating film;

[0034] Based on the focused ion beam etching system, using a helium source, a 10um aperture, and a beam size of about 2pA, using its precision processing performance, a nanohole array is prepared on a hard substrate coated with a precious metal film; among them, the focused ion beam dose About...

Embodiment 2

[0046] 1. Preparation of a Bimetallic Crater Surface Enhanced Raman Scattering Substrate

[0047] Such as figure 1 The process flow for the preparation of the enhanced Raman scattering substrate on the surface of the bimetallic crater is shown. On the surface of hard substrates such as silicon and glass cleaned by plasma or chemical methods, the magnetron sputtering method is used to plate gold and silver films in sequence. , the thickness of the gold film is 100 nanometers, and the thickness of the silver film is 50 nanometers; wherein, in order to improve the adsorption of the gold film on the substrate surface, 3 nanometers of chromium need to be plated before the gold plating film;

[0048] Based on the focused ion beam etching system, using a helium source, a 10um aperture, and a beam size of about 2pA, using its precision processing performance, a nanohole array is prepared on a hard substrate coated with a precious metal film; among them, the focused ion beam dose Abou...

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Abstract

The invention relates to a method for researching the allelopathic action mechanism of blue-green algae population competition based on surface-enhanced Raman scattering spectroscopy, and belongs to the technical field of population research. The method comprises the following steps: S1: preparation of bimetallic crater surface-enhanced Raman scattering substrate; S2: mixed culture of blue-green algae cells; S3: surface-enhanced Raman scattering imaging characterization of algal cells; S4: establishment of algae cell species level Discriminant analysis model of surface-enhanced Raman scattering spectroscopy; S5: Research on allelopathy of blue-green algae population competition based on surface-enhanced Raman scattering spectroscopy. The double-metal crater nanopore array surface-enhanced Raman scattering substrate disclosed by the present invention can directly enhance the fingerprint characteristics of algae cells, avoid the toxicity of traditional metal nanoparticles mixed culture or intracellular synthetic nanoparticles to cells, and reduce the Influence of substrates on the study of the mechanism of allelopathic action of algal toxins.

Description

technical field [0001] The invention belongs to the technical field of population competition mechanism research, and relates to a research method for the allelopathic mechanism of blue-green algae population competition based on surface-enhanced Raman scattering spectroscopy. Background technique [0002] At present, at home and abroad, it is mainly through the statistical analysis of natural water physiological parameters and dominant algal species to study the competitive relationship between different algal species in the algae community, or through experiments to simulate the influence of different physiological environments in the water body on the growth state of the algal group cells, or to study the algae cell growth status. The promotion mechanism of secretion of toxins / polysaccharides, etc. on algal population size, algal species interaction and community dominance, and the mechanism of planktonic algae community succession and water bloom are described. Among the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 何石轩王德强谢婉谊方绍熙周大明
Owner CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI