Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

High-efficiency single-cell capture and rapid single-cell secretory protein detection platform and method

A secreted protein detection platform technology, applied in chemical instruments and methods, biochemical equipment and methods, tissue cell/virus culture devices, etc., can solve the problem of low sensitivity of single cell secreted protein detection and large volume of secreted protein detection cavity , low single cell capture efficiency and other problems, to achieve the effect of small size, high detection sensitivity and fast capture

Active Publication Date: 2019-07-09
厦门德运芯准科技有限公司
View PDF10 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems of the existing single-cell secreted protein detection platform, such as low single-cell capture efficiency, large volume of secreted protein detection chamber, low detection sensitivity of single-cell secreted protein, serious waste of reagents, etc., the present invention proposes a single-cell high-efficiency capture Efficiency, small volume of secreted protein detection chamber, high sensitivity of secreted protein detection, rapid single-cell secreted protein capture, reagent-saving efficient single-cell capture and rapid single-cell secreted protein detection platform

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High-efficiency single-cell capture and rapid single-cell secretory protein detection platform and method
  • High-efficiency single-cell capture and rapid single-cell secretory protein detection platform and method
  • High-efficiency single-cell capture and rapid single-cell secretory protein detection platform and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] see Figure 1 to Figure 14 .High-efficiency single-cell capture and rapid single-cell secreted protein detection platform, including single-cell capture and droplet incubation microfluidic chip, secreted protein capture glass plate, chip fixture three parts; among them,

[0054] 1. See Figure 11 , the single cell capture and droplet incubation microfluidic chip includes: an isolation valve layer 23 on the upper layer and a single cell capture and droplet incubation layer 24 on the lower layer; the single cell capture and droplet incubation layer is connected end to end by multiple experimental units Each experimental unit includes a straight cell flow channel 7 located at the left and right ends of the cell flow channel. The two straight flow channels are connected by a U-shaped or arc-shaped cell flow channel 11. The left end of the cell capture chamber 6 is connected to the U Shaped or arc-shaped cell flow channel 11 is connected, the right end is connected with the...

Embodiment 2

[0076] (1) Thermal bonding of single cell capture and droplet incubation layer PDMS chip, isolation valve layer PDMS chip

[0077]First use an upright microscope to align the single cell capture and droplet incubation layer with the isolation valve layer and stick them together, then place the chip in a 60-degree oven to heat for 20 minutes, then peel it off from the silicon wafer template, and Holes are punched at the entrance and exit of the chip channel, and the microfluidic chip for single cell capture and droplet incubation is completed.

[0078] (2) Modification of single cell capture and droplet incubation microfluidic chip

[0079] Use electronic fluoride solution 1720 to modify the channel of the chip. After modification, heat it in an oven for 1 hour to ensure that the solvent is completely evaporated, and then place the chip in PBS for boiling. After boiling, soak the chip in PBS and wait for the experiment. take out.

[0080] (3) Modification of single-cell secre...

Embodiment 3

[0084] Example 3 Modification of single-cell secreted protein capture glass plate

[0085] Fabrication of modified channel microfluidic PDMS chips, such as Figure 5 As shown, and punch holes at its entrance and exit; put the chip in absolute ethanol for 10 minutes, then put the chip in ultrapure water for 10 minutes, take it out and use nitrogen to dry the surface moisture, and put the chip in a clean place. Put it in a 80-degree oven and heat it for 30 minutes to dry; use adhesive tape to remove the residual impurities on the surface of the modified chip channel, attach the chip to the poly-lysine glass plate naturally, and place it in an 80-degree oven for heating Thermal bonding was performed for 2 hours; after it cooled down, BSA-FITC, IL-8 capture antibody, MCP-1 capture antibody, TNF-a capture antibody, and MIP-1b capture antibody were respectively passed through its 5 modification channels, 1.5 microliters each were used to modify the secreted protein capture antibody...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Lengthaaaaaaaaaa
Login to View More

Abstract

The invention discloses a high-efficiency single-cell capture and rapid single-cell secretory protein detection platform and method. The detection platform is composed of three parts of a single-cellcapture and liquid drop incubation micro-fluidic control chip, a secretory protein capture glass plate and a chip clamp; and the single-cell capture and liquid drop incubation micro-fluidic control chip is provided with two layers of structures, namely a single-cell capture and liquid drop incubation layer and an isolation valve layer. The platform can perform single-cell capture with high efficiency and fastness, micro-droplets containing single cells are rapidly and stably generated through the addition of the isolation phase, and long-term liquid drop incubation can be carried out; and meanwhile, the high activity of the single cells is ensured, the chip is combined with a detection antibody array glass plate of secretory protein, and the high-flux, rapid and high-sensitivity detectionof the single-cell secretory protein can be realized.

Description

technical field [0001] The invention relates to a platform for high-efficiency single-cell capture and rapid single-cell secreted protein detection, and belongs to the technical field of microfluidic single-cell analysis methods. Background technique [0002] The traditional cell research method is based on the group research of a large number of cells, and the results obtained are often the average results of all cells. However, studies have shown that the gene expression and phenotype of different cell individuals have certain differences in the same type of cells (Zhang, Y et al. (2014) Journal of the American Chemical Society136:15257), which is called cell heterogeneity. qualitative. The experimental results obtained from group experiments will cover up the heterogeneity of cells, which will affect the treatment, prognosis and chance of recurrence of cancer. Single-cell research refers to dispersing and studying cells in independent experimental units to obtain indepe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/68G01N33/543C12M3/00B01L3/00
CPCB01L3/502761B01L2200/10B01L2300/0861B01L2300/0887B01L2400/0403B01L2400/06C12M23/16G01N33/543G01N33/6803
Inventor 杨朝勇黄培烽张明霞莫诗朱志周雷激
Owner 厦门德运芯准科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com