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Method for extracting taxillus chinensis glycosides from rosa chinensis flowers

A technology of wide parasitic glycoside and Chinese rose, which is applied in the field of plant extraction and separation, can solve the problems of no extraction and separation of wide parasitic glycoside, and achieve the effects of beneficial multiple utilization, stable chemical properties, and easy elution and regeneration

Active Publication Date: 2019-07-12
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is no report about the extraction and isolation of broad parasitin from rose flowers

Method used

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  • Method for extracting taxillus chinensis glycosides from rosa chinensis flowers
  • Method for extracting taxillus chinensis glycosides from rosa chinensis flowers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for extracting broadparasitin from Chinese rose, comprising the steps of:

[0027] 1) Take 2kg of dried rose flowers, crush them, and degrease them with petroleum ether for 3 times at room temperature. Extraction 3 times, each cold soaking extraction time is 7 days, 3 days, 3 days respectively, the extracts are combined and concentrated to obtain 600 g of total ethanol extract; among them, the addition ratio of dried rose flower and petroleum ether is 1 kg: 5L, the addition ratio of residue and ethanol is 1kg:5L;

[0028] 2) Dissolve the total ethanol extract (600 g) with an appropriate amount of methanol and mix the sample with a D101 macroporous resin column (600 g), and perform elution with water, 20% ethanol, and 40% ethanol in sequence through D101 macroporous resin column chromatography , evaporate the solvent to obtain 40% ethanol elution fraction;

[0029] 3) The fraction eluted with 40% ethanol was eluted by atmospheric silica gel column chromatograp...

Embodiment 2

[0036] A method for extracting broadparasitin from Chinese rose, comprising the steps of:

[0037] 1) Take 2kg of dried rose flowers, crush them, and degrease them with petroleum ether for 4 times at room temperature. Extracted 2 times, each cold soaking extraction time was 8 days and 3 days respectively, the extracts were combined and concentrated to obtain 510 g of total ethanol extract; among them, the addition ratio of dried rose flower and petroleum ether was 1 kg: 6 L, and the residue The addition ratio with ethanol is 1 kg: 6 L;

[0038] 2) Dissolve the total ethanol extract (510 g) with an appropriate amount of methanol and mix the sample with a D101 macroporous resin column (510 g). After chromatography on a D101 macroporous resin column, wash with water, 20% ethanol, and 40% ethanol in sequence. Remove and evaporate the solvent to obtain 40% ethanol elution fraction;

[0039]3) The fraction eluted with 40% ethanol was eluted by atmospheric silica gel column chromat...

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Abstract

The invention belongs to the technical field of plant extraction and particularly relates to a method for extracting taxillus chinensis glycosides from rosa chinensis flowers. The method specificallycomprises the steps of 1, smashing the dried rosa chinensis flowers, performing cold leaching degreasing with petroleum ether at the room temperature, evaporating the petroleum ether in residues, thenadding ethanol, performing cold leaching extraction, and concentrating an extracted solution to obtain a final ethanol extract; 2, dissolving the final ethanol extract in methanol, stirring the sample, sequentially using water, 20% ethanol and 40% ethanol for elution through a D101 macro-porous resin column, and volatilizing the solvent to obtain 40% ethanol elution components; 3, detecting and combining the 40% ethanol elution components through normal pressure silica gel column chromatography and silica gel thin-layer chromatography to obtain 12 components, and sequentially marking the components from Fr.4-1 to Fr.4-12 according to an ascending order of the polarity of the obtained components; 4, purifying the fourth component Fr.4-4 through gel column chromatography and high performance liquid chromatography to obtain a pure component, namely the taxillus chinensis glycoside. By means of the method, the taxillus chinensis glycoside contained in the dried rosa chinensis flowers canbe quickly and effectively separated out, and the extraction yield of the taxillus chinensis glycoside is high.

Description

technical field [0001] The invention belongs to the technical field of plant extraction and separation, and in particular relates to a method for effectively and rapidly extracting wide parasitic glycosides from rose flowers. Background technique [0002] Chinese rose is Rosaceae rose plant rose Rosa chinensis Dried flowers of Jacq. The main components of rose flower are flavonoids such as quercetin and kaempferol; other steroid components such as oleanolic acid, β -Sitosterol, campesterol, cycloeucalyptol, etc., and volatile oil components such as gallic acid, etc. Rose has a variety of pharmacological effects, including inhibiting platelet aggregation, reducing vascular permeability, antifungal and antiviral, antioxidative, and enhancing the body's immunity. [0003] At present, there is no report on the extraction and isolation of broadparasitin from rose flowers. Contents of the invention [0004] The purpose of the present invention is to overcome the defects of ...

Claims

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Application Information

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IPC IPC(8): C07H17/07C07H1/08
CPCC07H17/07C07H1/08
Inventor 马常阳康文艺刘振花魏金凤李昌勤呼谧允牛云
Owner HENAN UNIVERSITY