Streptomyces with double functions of preventing diseases and killing pests, application and culture method thereof, and biocontrol agent

A dual-action, cultivation method technology, applied in biocides, fungicides, insecticides, etc., can solve problems such as inability to inhibit plant pests, and achieve strong antibacterial activity

Active Publication Date: 2019-07-19
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although some actinomycetes that have antagonistic effects on plant pathogenic bacteria have been reported in the above-mentio

Method used

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  • Streptomyces with double functions of preventing diseases and killing pests, application and culture method thereof, and biocontrol agent
  • Streptomyces with double functions of preventing diseases and killing pests, application and culture method thereof, and biocontrol agent
  • Streptomyces with double functions of preventing diseases and killing pests, application and culture method thereof, and biocontrol agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Streptomyces with dual effects of disease prevention and insecticide in this example, the strain is Streptomyces (Streptomycessp.) MS001, the preservation number is CCTCC NO: M 2019266; preservation date: April 17, 2019, preservation unit: Chinese typical Culture Collection Center, deposit address: Wuhan, China. Wuhan University (Wuhan University Collection Center, Luojia Mountain, Wuchang, Wuhan City, Hubei Province).

[0041] Example 1 of the application of Streptomyces with dual effects of disease prevention and insecticide in antagonizing plant pathogenic bacteria

[0042] The strain used in this example is Streptomyces sp. MS001, and the deposit number is CCTCCNO:M2019266. The phytopathogens are Botrytis botrytis, Glososporum anthracnose, and / or Phomopsis appleus.

Embodiment 2

[0044] The strain used in this example is Streptomyces sp. MS001, and the deposit number is CCTCCNO:M2019266. The plant pathogenic bacteria are wheat sheath blight, wheat take-all, tomato cinerea, wheat scab, Clivia stem rot, and / or Nandina anthracnose.

[0045] Example 1 of the application of Streptomyces with dual effects of disease prevention and insecticide in suppressing plant pests

[0046] The strain used in this example is Streptomyces sp. MS001, and the deposit number is CCTCCNO:M2019266. Plant pests are cotton bollworm and / or monochamus larvae.

[0047] Embodiment 1 of the culture method of Streptomyces with dual effects of disease prevention and insecticide

[0048] In the method for cultivating Streptomyces with dual functions of disease prevention and insecticide in this embodiment, the culture medium used is Gao's No. 1 medium; the culture conditions are: temperature 20-32° C., pH=5-9. More preferably, the culture temperature is 23°C, pH=7.

[0049] From the ...

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Abstract

The invention relates to streptomyces with double functions of preventing diseases and killing pests, an application and a culture method thereof, and a biocontrol agent thereof, and belongs to the technical field of plant pest control. In the invention, an endophytic streptomyces MS001 with bacteriostatic activity is separated and obtained. The fermentation liquor has stronger bacteriostatic activity on Botryosphaeria dothidea, Colletotrichum gloeosporioides, Phomopsis mali, Rhizoctonia cerealis, Gaeumannomyces graminis, Botrytis cinerea, Fusarium graminearum, Fusarium oxysporum and Colletotrichum karstii, and the bacteriostatic objects have broad-spectrum property; and the fermentation liquor has the function of inhibiting the growth of plant pests such as helicoverpa armigera and monochamus alternatus larvae. Therefore, the streptomyces MS001 can prevent and control some diseases and pests on apple trees, garden plants, vegetables and food crops.

Description

technical field [0001] The invention relates to a streptomycete with dual functions of disease prevention and insecticide, its application, a cultivation method and a biocontrol agent, and belongs to the technical field of plant disease and insect pest control. Background technique [0002] Endophytic actinomycetes are widely distributed in the roots, stems, leaves, fruits and seeds of plants. A large number of studies have shown that actinomycetes isolated from plants have great application value. [0003] The Australian research team isolated more than 60 strains of actinomycetes from the roots of wheat, and screened out the strains for preventing and controlling wheat take-all disease (Gaeumannomyces graminis var. CM.Isolation and identification of actinobacteria from surface-sterilized wheat roots[J].Applied and Environmental Microbiology,2003,69(9):5603~5608.). Liang Yaping and others isolated 29 strains of endophytic actinomycetes from wild plants, among which the con...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00A01P7/04C12R1/465
CPCC12N1/20A01N63/00C12R2001/465C12N1/205A01N63/10
Inventor 周洲李永丽曲良建王亚红张梦珂常乐
Owner HENAN UNIV OF SCI & TECH
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