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Novel hyaluronic acid flowing gel and preparation method and application thereof

A technology of hyaluronic acid and gel, which is applied in pharmaceutical formulations, medical preparations containing active ingredients, medical science, etc., can solve the problems of anti-adhesion and poor wound healing, achieve good bioadhesion, and promote wound healing , Excellent biocompatibility effect

Active Publication Date: 2019-07-23
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In view of the above-mentioned defects of the prior art, the technical problem to be solved by the present invention is how to overcome the easy degradability of hyaluronic acid, and improve the injectability and biological safety of hyaluronic acid gel, thereby solving the problem of hyaluronic acid in the prior art. Hyaluronic acid is used for bone and joint treatment, postoperative anti-adhesion, wound healing and cosmetic filling, etc.

Method used

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  • Novel hyaluronic acid flowing gel and preparation method and application thereof
  • Novel hyaluronic acid flowing gel and preparation method and application thereof
  • Novel hyaluronic acid flowing gel and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Step 1: Dissolve 5g of hyaluronic acid powder (molecular weight: 500-2000kDa) in 500mL of water, stir until completely dissolved at room temperature, and prepare a hyaluronic acid solution;

[0041] Step 2: Use a homogenizer to disperse hyaluronic acid evenly in water, then slowly add 1M HCl to adjust the pH of the solution to pH<5. Divide the hyaluronic acid solution into centrifuge bottles and centrifuge to remove air bubbles in the solution. The hyaluronic acid solution after removing the air bubbles is divided into weighing bottles, and the lid is closed and sealed with a parafilm;

[0042] Step 3: Put the subpackaged solution obtained in Step 2 into a -20°C refrigerator and freeze for 3 days, then take out the weighing bottle and thaw for 6 hours, repeat the freezing and thawing process 3 times, and obtain an acidic freeze-thawed hyaluronic acid gel ;

[0043] Step 4: Transfer the acidic frozen-thawed hyaluronic acid gel obtained in step 3 to a dialysis bag and d...

Embodiment 2

[0047] Step 1: Dissolve 5g of hyaluronic acid powder (molecular weight: 500-2000kDa) in 500mL of water, stir until completely dissolved at room temperature, and prepare a hyaluronic acid solution;

[0048] Step 2: Use a homogenizer to uniformly disperse hyaluronic acid in water, and then slowly add 1M HCl to adjust the pH of the solution to be the same as that in Example 1. Divide the hyaluronic acid solution into centrifuge bottles and centrifuge to remove air bubbles in the solution. The hyaluronic acid solution after removing the air bubbles is divided into weighing bottles, and the lid is closed and sealed with a parafilm;

[0049] Step 3: Put the subpackaged solution obtained in Step 2 into a -20°C refrigerator and freeze for 6 days, then take out the weighing bottle and thaw for 6 hours, repeat the freezing and thawing process 3 times, and obtain an acidic freeze-thawed hyaluronic acid gel ;

[0050] Step 4: Transfer the acidic frozen-thawed hyaluronic acid gel obtaine...

Embodiment 3

[0054] Step 1: Dissolve 5g of hyaluronic acid powder (molecular weight: 500-2000kDa) in 500mL of water, stir until completely dissolved at room temperature, and prepare a hyaluronic acid solution;

[0055] Step 2: Use a homogenizer to uniformly disperse hyaluronic acid in water, and then slowly add 1M HCl to adjust the pH of the solution to be the same as that in Example 1. Divide the hyaluronic acid solution into centrifuge bottles and centrifuge to remove air bubbles in the solution. The hyaluronic acid solution after removing the air bubbles is divided into weighing bottles, and the lid is closed and sealed with a parafilm;

[0056] Step 3: Put the aliquoted solution obtained in Step 2 into a -20°C refrigerator and freeze for 12 days, then take out the weighing bottle and thaw for 6 hours, repeat the freezing and thawing process 3 times, and obtain an acidic freeze-thawed hyaluronic acid gel ;

[0057] Step 4: Transfer the acidic frozen-thawed hyaluronic acid gel obtained...

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Abstract

The invention discloses novel hyaluronic acid flowing gel and a preparation method and application thereof. The preparation method comprises the following steps of preparing acidic frozen and unfrozenhyaluronic acid gel; preparing neutral frozen and unfrozen hyaluronic acid gel; and performing freeze drying and breaking treatment on the neutral frozen and unfrozen hyaluronic acid gel and preparing the hyaluronic acid flowing gel. The prepared hyaluronic acid flowing gel is physical gel, is free from a cross-linking agent, cannot cause chemical substance residues, and cannot cause potential cytotoxicity. The novel hyaluronic acid flowing gel has the advantages of being favorable in biocompatibility, high in degradability resistance, injectable and the like, and can be applied to degenerative arthritis treatment, postoperative anti-adhesion, wound healing, cosmetology stuffing and the like.

Description

technical field [0001] The invention relates to the technical field of biomaterials, in particular to a preparation method and application of a novel hyaluronic acid flow gel. Background technique [0002] Hyaluronic acid (Hyaluronic acid, HA) is a linear polyanionic polysaccharide linked by D-glucuronic acid and N-acetylglucosamine through β-(1,4) and β-(1,3) glycosidic bonds. Hyaluronic acid is a biodegradable polysaccharide that can be degraded by hyaluronidase, which is widely present in mammalian tissues. In addition, hyaluronic acid can specifically bind to the CD44 receptor on the surface of chondrocytes, which is closely related to the functions of cell metastasis, proliferation, and differentiation. Therefore, hyaluronic acid is often used as a biomaterial for tissue engineering. However, the biggest disadvantage of hyaluronic acid is that it degrades rapidly in tissues. In order to reduce the degradation rate, cross-linking hyaluronic acid molecules to delay deg...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/50A61L27/52A61L15/28A61L15/42A61L15/44A61L31/04A61L31/14A61K31/728A61K9/06A61P19/02A61P19/08A61P29/00A61P17/02
CPCA61K9/06A61K31/728A61L15/28A61L15/42A61L15/44A61L27/20A61L27/50A61L27/52A61L31/042A61L31/14A61L31/145A61L2300/236A61L2300/412A61L2400/06A61P17/02A61P19/02A61P19/08A61P29/00C08L5/08
Inventor 张洪斌蔡志祥
Owner SHANGHAI JIAO TONG UNIV
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