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Pseudomonas Lebanon strain my and its application in remediation of heavy metal-contaminated saline soil

A technology of pseudomonas and heavy metals, which is applied to plant growth-promoting bacteria, Pseudomonas baneni strain MY and its application in the remediation of heavy metal-contaminated saline soil, can solve the problem of rare plant remediation of heavy metal-contaminated saline soil and other problems, to achieve the effect of improving extraction efficiency, promoting phytoremediation efficiency, and good application prospects

Active Publication Date: 2021-06-11
马莹 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are very few studies on phytoremediation of heavy metal-contaminated saline soils

Method used

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  • Pseudomonas Lebanon strain my and its application in remediation of heavy metal-contaminated saline soil
  • Pseudomonas Lebanon strain my and its application in remediation of heavy metal-contaminated saline soil
  • Pseudomonas Lebanon strain my and its application in remediation of heavy metal-contaminated saline soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Isolation, Identification and Characterization of Pseudomonas Lebanon MY (CGMCC No.15613)

[0026] 1.1 Test soil

[0027] Rhizosphere soil from clover growing on serpentinite-grown soil in the mountains of northeastern Portugal (41°46′30″N; 6°53′55″W). Its basic physical and chemical properties are: pH 7.4, organic matter 8.6 g kg -1 , total amount of copper 185 mg kg -1 , the total amount of cobalt 152 mg kg -1 , total amount of zinc 250 mg kg -1 , the total amount of chromium 2800 mg kg-1 , the total amount of nickel is 1945 mg kg -1 , the total amount of lead is 84 mgkg -1 . Fresh soil samples were passed through a 2 mm sieve and stored in the dark at 4 °C.

[0028] 1.2 Heavy metals tested

[0029] CdCl 2 , CrCl 2 、CuSO 4 、NiCl 2 , Pb(NO 3 ) 2 and ZnSO 4 All were purchased from Sigma Company (USA), and were of analytical grade.

[0030] 1.3 Culture medium type

[0031] 1) Luria-Bertani (LB) medium: containing 5 g yeast extract, 10 g pepton...

Embodiment 2

[0073] Example 2: The method of Pseudomonas Lebanon MY (CGMCC No. 15613) combined with phytoremediation of heavy metal polluted saline soil

[0074] 1.1 Test soil

[0075] Soil was collected from the Botanical Garden of the University of Coimbra, Portugal. The basic physical and chemical properties of the soil are: pH 7.4, organic matter 1.6%, cation exchange capacity (CEC) 1.5 meq (100g) -1 , electrical conductivity (EC) 0.3 dS m -1 , water-soluble nitrogen content 62.8mg kg -1 , water-soluble phosphorus content 18.2 mg kg -1 , water-soluble potassium content 70.5 mg kg -1 . The soil was sieved (2 mm) and sterilized at 100 °C for 1 h on three consecutive days. Add NiCl to the soil 2 solution to achieve a final concentration of 350 mg Ni kg -1 to prepare nickel-contaminated soil and place it in the greenhouse for two weeks (for heavy metal stabilization). Apply NaCl solution to the soil to achieve a final concentration of 4.6 g NaCl kg -1 for saline soil. To avoid o...

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Abstract

Pseudomonas Lebanon strain MY and its application in the remediation of heavy metal-contaminated saline soil, the strain has been preserved in the preservation unit designated by the State Intellectual Property Office, the preservation date is April 13, 2018, the name of the preservation unit: China Microbial Bacteria General Microbiology Center of Species Preservation Committee, deposit number: 15613. The rhizosphere growth-promoting bacteria have strong resistance to heavy metals and salt resistance, can significantly promote the growth of plants, reduce the toxicity of heavy metals and excessive salt ions to plants, and at the same time improve the absorption of heavy metals and salt ions in the soil by plants , to promote phytoremediation efficiency. Therefore, it can be directly used to improve the phytoremediation efficiency of saline soil or heavy metal-contaminated soil, and has a good application prospect in the bioremediation of heavy metal-contaminated saline soil.

Description

[0001] 1. Technical field [0002] The present invention relates to Pseudomonas Lebanon, in particular to a plant growth promoting bacterium (Plant growth promoting bacteria, PGPB) Pseudomonas Lebanon strain MY with strong heavy metal resistance and salt resistance and its application in heavy metal polluted saline soil Application in repair. [0003] 2. Background technology [0004] Soil heavy metal pollution is a major environmental problem facing the world. It not only seriously affects soil quality and soil fertility, but also endangers ecology, food safety, and human health. Compared with traditional restoration methods, phytoremediation has attracted widespread attention due to its advantages of environmental protection, high efficiency, and low cost. However, abiotic stress conditions induced by climatic factors are often encountered during plant growth, such as salinity, drought, and extreme temperature, which seriously hinder the growth of plants and their restoratio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/10B09C1/00
CPCB09C1/00B09C1/10
Inventor 马莹张长
Owner 马莹