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Pesticide content detection method for wine-making

A detection method and wine technology, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as the pollution of dead grape leaves and achieve the effect of eliminating interference

Inactive Publication Date: 2019-08-02
安徽年丰珍盛农业发展股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a pesticide content detection method for wine brewing that can solve the problem of grape dead leaf pollution, easily crush grapes, extract and eliminate interference, and accurately detect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for detecting pesticide content in wine brewing, comprising the following steps:

[0034] Step 1. Soak the grapes in liquid nitrogen for 5 minutes, take them out, put them into a ball mill, and inject an appropriate amount of liquid nitrogen into the ball mill, then grind the grape balls into fine powder, and use a 50-mesh sieve to screen the grapes below 50 mesh Grape powder, and weigh 5g of grape powder mass below 50 mesh, put 5g of grape powder into the beaker;

[0035] Step 2: Add 50mL of saturated sodium chloride solution to the above beaker, stir with a magnetic stirrer at 500r / min for 15 minutes, and then put it in a refrigerator at 0°C for 30 minutes;

[0036] Step 3, take out the sodium chloride grape solution from the refrigerator, and filter through the built-in two-layer medium-speed filter paper of the Buchner funnel to obtain the filtrate;

[0037] Step 4: Add 5 mL of chloroform to the above filtrate, then perform magnetic stirring at 1000r / min f...

Embodiment 2

[0042] A method for detecting pesticide content in wine brewing, comprising the following steps:

[0043] Step 1. Soak the grapes in liquid nitrogen for 10 minutes, take them out, put them into a ball mill, and inject an appropriate amount of liquid nitrogen into the ball mill, then grind the grape balls into fine powder, and use a 100-mesh sieve to screen the grapes below 100 mesh Grape powder, and weigh 5g of grape powder below 100 mesh, put 5g of grape powder into a beaker;

[0044] Step 2: Add 60mL of saturated sodium chloride solution to the above beaker, stir with a magnetic stirrer at 1000r / min for 25 minutes, and then put it in a refrigerator at 5°C for 40 minutes;

[0045] Step 3, take out the sodium chloride grape solution from the refrigerator, and filter through the built-in two-layer medium-speed filter paper of the Buchner funnel to obtain the filtrate;

[0046] Step 4: Add 8 mL of acetone to the above filtrate, then perform magnetic stirring at 1500r / min for 15...

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PUM

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Abstract

The invention discloses a pesticide content detection method for wine-making. The pesticide content detection method for wine-making comprises the steps of: (1), immersing by putting grapes into liquid nitrogen, after taking out, putting into a ball mill, then, grinding grape balls into fine powder, and, after screening, putting 5 g of grape powder into a beaker; (2), adding saturated sodium chloride solution into the beaker, and putting into a refrigerator; (3), performing suction filtration by putting two layers of medium-speed filter papers in a cloth type hopper, so that filtrate is obtained; (4), adding an organic solvent into the filtrate, then, centrifuging by pouring the mixed solution into a centrifugal tube, and taking out supernate; (5), putting the supernate into a freeze dryer, and freezing and drying at -20 to -10 DEG C for 2-8 h, so that a solute is obtained; (6), adding 25 mL of methanol into the solute, and performing ultrasonic oscillation, so that transparent solution is formed; and (7), detecting by injecting into a sampling valve of a liquid chromatograph by using a pipette. The pesticide content detection method for wine-making disclosed by the invention has the characteristics of solving the grape withered leave pollution problem, easily crushing grapes, eliminating interference in extraction and performing accurate detection.

Description

technical field [0001] The invention relates to a pesticide content detection method for wine brewing, in particular to a pesticide content detection method for wine brewing which can solve the pollution problem of grape dead leaves, easily crush grapes, extract and eliminate interference, and accurately detect the pesticide content. Background technique [0002] Selenium-enriched grapes are not only delicious, but also have high nutritional value. The sugar content in mature berries is as high as 10% to 30%, mainly glucose, which can be directly absorbed by the human body, and also contains minerals such as calcium, potassium, phosphorus, and iron As well as a variety of vitamins, as well as a variety of essential amino acids, it is one of the fruits that people love; from the perspective of Chinese medicine, grapes are flat in nature, sweet and sour, and non-toxic. Chinese medicine believes that grapes can nourish blood, strengthen bones and muscles, strengthen the stomach ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 朱平
Owner 安徽年丰珍盛农业发展股份有限公司