Colloid dressings that promote wound healing
A wound healing and xanthan gum technology, applied in the biological field, can solve the problems of no obvious promotion of wound healing, heavy dressing change workload, and easy damage to new tissues.
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Embodiment 1
[0174] Embodiment 1, the acquisition of β-glucan
[0175] 1. Obtaining the fermentation broth of Schizophyllum strains
[0176] The fermented liquid in the following examples was fermented by Schizophyllum commune (Schizophyllum commune Fr-1003, catalog: 38548 TM , purchased from the Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences), the details are as follows:
[0177] (1) Activation of Schizophyllum strains: 200g / L of potatoes, 30g / L of glucose, 10g / L of sodium chloride, and 20g / L of agar were made into a plate medium, and the strains of Schizophyllum were inoculated into the plate medium Above, cultivated in a constant temperature incubator at 25°C for 7 days to obtain flat plate mycelium;
[0178] (2) Seed activation: put potato starch 100g / L, glucose 40g / L, yeast extract 2g / L, yeast extract powder 2g / L, and water into a shaker flask with a liquid volume of 1 / 3. Inoculate the shake flask with the plate mycelium obtained in step ...
Embodiment 2
[0221] Example 2 Dissolution experiment of xanthan gum and β-glucan composition
[0222] 1. Take five 500ml small beakers and add 100ml of the 0.5% Schizophyllum β-glucan aqueous solution prepared in Example 1 respectively, marked as ①, ②, ③, ④ and ⑤;
[0223] 2. Add 0.05, 0.1, 0.2, and 0.5 g of xanthan gum powder into ②, ③, ④ and ⑤ small beakers respectively, dissolve and stir evenly;
[0224] 3. Add 200ml of absolute ethanol to each beaker for alcohol precipitation;
[0225] 4. Take out the sediment and dry it at 60°C;
[0226] 5. After 24 hours, take out and crush;
[0227] 6. ①, ②, ③, ④, ⑤ redissolve each solid sample in 100ml water;
[0228] 7. Observe the water solubility of each sample.
[0229] The result is as figure 2 As shown, the combination of xanthan gum and Schizophyllum β-glucan has better water solubility and higher solubility than that of Schizophyllum β-glucan alone. As the concentration of xanthan gum increases, the water solubility of the compositio...
Embodiment 3
[0230] Example 3 Synergistic thickening experiment of xanthan gum and β-glucan
[0231] 1. Take six 500ml small beakers and add 100ml of the 0.5% Schizophyllum β-glucan aqueous solution prepared in Example 1 respectively, marked as ①, ②, ③, ④, ⑤ and ⑥;
[0232] 2. Add 0.05, 0.1, 0.15, 0.2, 0.3g of xanthan gum powder into ②, ③, ④, ⑤ and ⑥ small beakers respectively, dissolve and stir evenly;
[0233] 3. Check the viscosity and light transmittance of each sample.
[0234] The results are shown in Table 1 below. As the concentration of xanthan gum in 0.5% Schizophyllum β-glucan increases, the synergistic increase rate of viscosity of the composition also increases, for example, when the concentration of xanthan gum is 0.3%, the composition thickens synergistically The effect reached 89.7%. The above results are sufficient to prove that the combination of xanthan gum and Schizophyllum β-glucan has a synergistic thickening effect.
[0235] Table 1 Synergistic thickening effect ...
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