Method for preparing fermented soybean milk powder through lactobacillus fermentum, prepared fermented soybean milk powder and application

A technology of fermenting Lactobacillus and fermenting soybean milk, which is applied in the preparation of fermented soybean milk powder, fermented soybean milk powder and application fields, can solve the problems of oral flora imbalance, lack of fermented products, and limited, etc., and achieve good stability and significant inhibitory effect Effect

Active Publication Date: 2019-08-09
BRIGHT DAIRY & FOOD
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  • Abstract
  • Description
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Problems solved by technology

[0004] At present, most of the research reports on dental caries treatment focus on controlling or reducing the number of Streptococcus mutans in the oral cavity. However, a healthy oral environment is generally in a state where harmful bacteria and beneficial bacteria reach a balance. Excessive reduction of harmful bacteria will Bring the imbalance of oral flora, and then cause other adverse reactions of the human body
In addition, some clinically used bactericidal drugs against Streptococcus mutans, such as silver ammonium nitrate and ot...

Method used

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  • Method for preparing fermented soybean milk powder through lactobacillus fermentum, prepared fermented soybean milk powder and application
  • Method for preparing fermented soybean milk powder through lactobacillus fermentum, prepared fermented soybean milk powder and application
  • Method for preparing fermented soybean milk powder through lactobacillus fermentum, prepared fermented soybean milk powder and application

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preparation example Construction

[0027] In a specific embodiment, a method for preparing fermented soybean milk powder is provided, comprising the following steps: inoculating Lactobacillus fermentum (Lactobacillus fermentum) CGMCC No.17321 in soybean milk for fermentation, and obtaining fermented soybean milk powder after freeze-drying .

[0028]The preparation method in the above-mentioned technical scheme adopts Lactobacillus fermentum (Lactobacillus fermentum) CGMCC No.17321 for the first time, ferments with soybean milk as a medium, and prepares fermented soybean milk powder with Streptococcus mutans biofilm inhibitory activity, which discloses Lactobacillus fermentum ( Lactobacillus fermentum) CGMCC No.17321 fermented soybean milk powder has a new application of anti-caries. Compared with other strains that can produce fermented soybean milk powder, the fermented soybean milk powder prepared by CGMCC No.17321 has a more significant inhibitory effect on the formation of Streptococcus mutans biofilm, and ...

Embodiment 1

[0044] 1. Materials and methods

[0045] (a) Preparation of Lactobacillus fermentum seeds (fermentation strains): Dissolve the lyophilized powder of Lactobacillus fermentum CGMCC No.17321 with a small amount of sterile distilled water, and use an inoculation loop to take a ring and streak it on the MRS solid medium (Merck Co. Germany), take out after anaerobic culture at 37°C for 24 hours, pick a single colony with an inoculation loop and put it into 1mL MRS liquid (Merck Co. Germany), use a vortex shaker to evenly disperse the colony in the liquid medium, Take it out after 24 hours of anaerobic culture at 37°C, inoculate 50 mL of MRS liquid with 2% (v / v) inoculum, cultivate at 37°C for 24 hours, centrifuge the culture at 9,000 rpm for 10 minutes, discard the supernatant, and wash the bacteria with sterile distilled water After 2 times, suspend with sterile distilled water of the original culture volume to obtain seeds for fermentation, and the bacterial concentration of the s...

Embodiment 2

[0058] 1. Materials and methods

[0059] (a) Preparation of Lactobacillus fermentum seed (fermentation strain) and Streptococcus mutans bacterial suspension: Same as Example 1.

[0060] (b) Preparation of soymilk: After weighing the dried red bean, add five times the mass of water and soak at 37°C for 8 hours, discard the water, add a certain proportion of wet beans and water to the soymilk machine to obtain the desired Soybean milk with solid content was sterilized at 121°C for 20 minutes to obtain aseptic red bean soymilk with specified solid content.

[0061] (c) Determination of the inhibitory rate and IC50 of fermented soybean milk powder to Streptococcus mutans biofilm formation: Same as Example 1.

[0062] 2. Preparation of fermented soybean milk powder

[0063] Lactobacillus fermentum (Lactobacillus fermentum) CGMCC No.17321 was aseptically inoculated into red bean milk with a solid content of 9% (w / w) at an inoculum size of 1% (v / v), cultured at 15°C for 48 hours, a...

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Abstract

The invention specifically discloses a method for preparing fermented soybean milk powder through lactobacillus fermentum, the prepared fermented soybean milk powder and application. The method comprises the following steps that the lactobacillus fermentum CGMCC No.17321 is inoculated in soybean milk for fermentation, and freezing drying is conducted to obtain the fermented soybean milk powder. The method for preparing the fermented soybean milk powder through the lactobacillus fermentum, the prepared fermented soybean milk powder and the application have the advantages that the obtained fermented soybean milk powder can significantly reduce the formation quantity of biofilms of streptococcus mutans, and it is disclosed that the fermented soybean milk powder of lactobacillus fermentum CGMCC No.17321/lactobacillus fermentum CGMCC No.17321 and lactobacillus plantarum ATCC 14917 (purchased from ATCC) has new application in dental caries resistance; compared with other strains capable of preparing soybean milk powder and other fermentation culture mediums, the obtained fermented soybean milk powder has a more remarkable effect of inhibiting the formation of the biofilms of the streptococcus mutans, and the stability is good.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of fermented soybean milk powder. In addition, the present invention also relates to a method for preparing fermented soybean milk powder from Lactobacillus fermentum, and the prepared fermented soybean milk powder and its application. Background technique [0002] Dental caries, commonly known as caries, tooth decay, is a bacterial disease, can be secondary to pulpitis and periodontitis, and even cause alveolar bone and jaw inflammation. If it is not treated in time, the lesion will continue to develop, forming cavities, and eventually the crown will be completely destroyed and disappeared. The final result of its development is tooth loss. Dental caries is characterized by high incidence and wide distribution. The disease is the main common disease of the oral cavity and one of the most common human diseases. The World Health Organization has list...

Claims

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Application Information

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IPC IPC(8): A23C11/10C12N1/20C12R1/225A23L11/50A23L11/65
CPCA23C11/106C12R2001/225C12N1/205
Inventor 韩瑨吴正钧王晓花冯华峰
Owner BRIGHT DAIRY & FOOD
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