Kit for detecting polymorphism of gene SCN1A related to levetiracetam drug administration by utilizing high resolution melting curve method and method of kit

A technology of high-resolution melting and detection method, which is applied in the field of detection of levetiracetam drug-related gene SCN1A polymorphism, can solve the problems of differences in the efficacy of LEV, and achieve the effect of simple operation and small sample volume

Pending Publication Date: 2019-08-09
SHANGHAI PASSION BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

SCN1A gene rs2298771 mutation leads to differences in the efficacy of LEV

Method used

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  • Kit for detecting polymorphism of gene SCN1A related to levetiracetam drug administration by utilizing high resolution melting curve method and method of kit
  • Kit for detecting polymorphism of gene SCN1A related to levetiracetam drug administration by utilizing high resolution melting curve method and method of kit
  • Kit for detecting polymorphism of gene SCN1A related to levetiracetam drug administration by utilizing high resolution melting curve method and method of kit

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Experimental program
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Embodiment 1

[0028] Upstream primer: 5'-CAA GAA AGA CAG TTG TAT GTC CAA TC-3' (SEQ ID NO.2);

[0029] Downstream primer: 5'-ACA CTG CTG CCA GTT CCT ATA CC-3' (SEQ ID NO.3);

[0030] 1. DNA extraction

[0031] 1.1 The preparation and inspection of reagent materials before the experiment are as follows:

[0032] (1) Check the shelf life of the kit and ensure that ethanol has been added to Wash Buffer 1 and 2, and tick the corresponding mark on the bottle; (2) Isopropanol (if not available, it can be replaced by absolute ethanol) and 75% ethanol ; (3) 1.5mL Eppendorf tubes and various pipette tips within the validity period of autoclaving.

[0033] 1.2 Take out the EDTA anticoagulant tube containing whole blood from the refrigerator at 4°C, and mix it upside down several times;

[0034] 1.3 Make a mark on the 1.5mL Eppendorf tube corresponding to the unique identification of the specimen;

[0035] 1.4 Pipette 900μl Cell Lysis Solution into a sterilized 1.5mL Eppendorf tube;

[0036] 1.5 ...

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Abstract

The invention discloses a kit for detecting polymorphism of a gene SCN1A related to levetiracetam drug administration by utilizing a high resolution melting curve method. The kit is characterized by comprising detection primers for a polymorphic site rs2298771 of the gene SCN1A, and nucleotide sequences of the primers are shown as below: the nucleotide sequence of the SCN1A upstream primer is 5'-CAA GAA AGA CAG TTG TAT GTC CAA TC-3'; the nucleotide sequence of the SCN1A downstream primer is 5'-ACA CTG CTG CCA GTT CCT ATA CC-3'; other parts contained in the kit are conventional reagents of an HRM analysis kit. The invention further discloses a detection method of the kit for detecting the polymorphism of the gene SCN1A related to the levetiracetam drug administration by utilizing the high resolution melting curve method. The kit is suitable for quickly detecting the individualized levetiracetam drug administration gene and can be widely applied to gene detection made for clinical individualized levetiracetam drug administration schemes.

Description

technical field [0001] The invention belongs to the field of gene detection, and in particular relates to a kit and a detection method for detecting the polymorphism of levetiracetam drug-related gene SCN1A by using a high-resolution melting curve method. Background technique [0002] Levetiracetam (Levetiracetam, Lev, trade name: Keppra, Kaiplan) was approved by the US FDA in 1999 for partial seizures in adults with epilepsy. Its oral tablet and solution were approved in 2005 for the adjunctive treatment of partial-onset seizures in children 4 years and older. The study found that the prominent vesicle protein SV2A in the brain is the site of action of the antiepileptic drug LEV. That is, LEV binding modifies the function of the SV2 protein to provide an anticonvulsant effect. [0003] Adjuvant therapy with LEVs has been shown to be both effective and well tolerated in the control of refractory partial-onset seizures in adults in extensive clinical trials. SCN1A gene rs2...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6858
CPCC12Q1/6858C12Q2531/113C12Q2527/107
Inventor 何小明钱怡孙子奎
Owner SHANGHAI PASSION BIOTECHNOLOGY CO LTD
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