Oriented conductive collagen hydrogel, biomimetic conductive nerve scaffold material and preparation method of oriented conductive collagen hydrogel and biomimetic conductive nerve scaffold material

A scaffold material and conductive glue technology, applied in the fields of biomedical materials and biomedical engineering, can solve the problems of limited cell adhesion sites, poor compatibility, and difficult to achieve conductive hydrogels, and achieve the promotion of elongation and nerve related problems. Functional expression, good biocompatibility, good application prospects

Active Publication Date: 2019-08-16
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method of preparing conductive hydrogel achieves three-dimensional encapsulation of cells, due to the limited cytocompatibility of HA, its limited cell adhesion sites, and the lack of oriented microstructure inside this conductive hydrogel, it cannot Well mimics the directional structures within native neural tissue, so its use in neural tissue engineering is limited
[0005] In summary, the currently reported conductive hydrogels are difficult to achieve three-dimensional encapsulation of cells, poor compatibility or lack of oriented microstructure, which makes them difficult to apply to neural tissue engineering research.

Method used

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  • Oriented conductive collagen hydrogel, biomimetic conductive nerve scaffold material and preparation method of oriented conductive collagen hydrogel and biomimetic conductive nerve scaffold material
  • Oriented conductive collagen hydrogel, biomimetic conductive nerve scaffold material and preparation method of oriented conductive collagen hydrogel and biomimetic conductive nerve scaffold material
  • Oriented conductive collagen hydrogel, biomimetic conductive nerve scaffold material and preparation method of oriented conductive collagen hydrogel and biomimetic conductive nerve scaffold material

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Embodiment 1

[0046] The steps of preparing the oriented conductive collagen hydrogel in this embodiment are as follows:

[0047] (1) Preparation of PPy nanoparticles

[0048] Disperse 1.5g of PVA (molecular weight: 31000) in 20mL of deionized water, and then place it in a 60°C oven and heat it for 30min to accelerate its dispersion to obtain the first dispersion, then take it out and cool it to room temperature. Then 1.2434g oxidant FeCl 3 ·6H 2 O was added to the first dispersion, stirred until the oxidant was fully dispersed, and then left to stand for 1h to obtain FeCl 3 A second dispersion with a concentration of 0.23 mol / L. Then, under stirring conditions at 5°C, 140 μL of the conductive monomer Py was added dropwise to the second dispersion (Py concentration was 0.1 mol / L), and then the stirring reaction was continued at 5°C for 4 hours, and then deionized water was used to remove the conductive monomer by centrifugal washing. For soluble impurities, determine the concentration o...

Embodiment 2

[0054] The steps of preparing the oriented conductive collagen hydrogel in this embodiment are as follows:

[0055] (1) Preparation of PPy nanoparticles

[0056] Disperse 1.5g of PVA (molecular weight: 31000) in 20mL of deionized water, and then place it in a 60°C oven and heat it for 30min to accelerate its dispersion to obtain the first dispersion, then take it out and cool it to room temperature. Then 1.2434g oxidant FeCl 3 ·6H 2 O was added to the first dispersion, stirred until the oxidant was fully dispersed, and then left to stand for 1h to obtain FeCl 3 A second dispersion with a concentration of 0.23 mol / L. Then, under stirring conditions at 5°C, 140 μL of the conductive monomer Py was added dropwise to the second dispersion (Py concentration was 0.1 mol / L), and then the stirring reaction was continued at 5°C for 4 hours, and then deionized water was used to remove the conductive monomer by centrifugal washing. For soluble impurities, determine the concentration o...

Embodiment 3

[0062] The steps of preparing the oriented conductive collagen hydrogel in this embodiment are as follows:

[0063] (1) Preparation of PPy nanoparticles

[0064] Disperse 1.5g of PVA (molecular weight: 31000) in 20mL of deionized water, and then place it in a 60°C oven and heat it for 30min to accelerate its dispersion to obtain the first dispersion, then take it out and cool it to room temperature. Then 1.2434g oxidant FeCl 3 ·6H 2 O was added to the first dispersion, stirred until the oxidant was fully dispersed, and then left to stand for 1h to obtain FeCl 3 A second dispersion with a concentration of 0.23 mol / L. Then, under stirring conditions at 5°C, 140 μL of the conductive monomer Py was added dropwise to the second dispersion (Py concentration was 0.1 mol / L), and then the stirring reaction was continued at 5°C for 4 hours, and then deionized water was used to remove the conductive monomer by centrifugal washing. For soluble impurities, determine the concentration o...

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Abstract

The invention discloses an oriented conductive collagen hydrogel, a biomimetic conductive nerve scaffold material and a preparation method of the oriented conductive collagen hydrogel and the biomimetic conductive nerve scaffold material. The mixture of natural biological macromolecular collagen and polymer nanoparticles with excellent biocompability is used as a raw material, and oriented conductive collagen hydrogel fiber is prepared by a coaxial microfluidic chip with a PEG (polyethylene glycol) buffer solution; the biomimetic conductive nerve scaffold material with in situ loading of cellsis obtained by adding the cells in the process of hydrogel preparation. The hydrogel fiber prepared has the advantages of electrical conductivity matching with natural nerve tissue, similar mechanical properties, good biocompatibility, and can be arranged along the direction of the hydrogel fiber on a micro and nano scale, and can simulate the directional structure in the natural nerve tissue; the biomimetic conductive nerve scaffold material prepared can stimulate the arrangement of neurous in the nerve tissue along the direction of the nerve fiber and conduction of electrical signals alongaxons, and shows a good application prospect in the field of neural tissue engineering.

Description

technical field [0001] The invention belongs to the technical fields of biomedical materials and biomedical engineering, and relates to hydrogels, in particular to an oriented conductive collagen hydrogel and a preparation method thereof. Background technique [0002] Nerve tissue is a special tissue with a multi-level directional structure. It is composed of nerve bundles with oriented structures. Directed growth along the myelin sheath. [0003] In nervous tissue, neurons communicate through electrical signals, which play a vital role in the survival, differentiation and functional expression of neurons. In addition to supporting cell growth, the extracellular matrix of neurons acts as a medium for the transmission of electrical signals between neurons and between neurons and substrates. Its electrical conductivity directly affects the efficiency of electrical signal transmission. Therefore, biocompatible conductive matrices have excellent application prospects in the fi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/52A61L27/16A61L27/38A61L27/50
CPCA61L27/16A61L27/383A61L27/50A61L27/52A61L2430/32C08L89/00C08L29/04
Inventor 范红松吴承恒陈素萍陈露孙静罗红蓉
Owner SICHUAN UNIV
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