Nitrilase xinit1 and its coding gene and application

A technology of nitrilase and gene, applied in nitrilase XiNit1 and its coding gene and application fields, to achieve the effects of high tolerance, wide reaction pH and high enzyme activity
CN110129304BInactive Publication Date: 2020-09-15CHINA UNIV OF PETROLEUM (EAST CHINA)
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHINA UNIV OF PETROLEUM (EAST CHINA)
Publication Date
2020-09-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses nitrilase XiNit1 and a coding gene and application thereof. Protein provided by the invention comes from Xinfangfangiasp, is nitrilase, is named as XiNit1 protein, is protein composed of an amino acid sequence shown as a sequence 1 in a sequence table and protein composed of an amino acid sequence shown as a sequence 3 in the sequence table. The invention further disclosesapplication of the XiNit1 protein as nitrilase and a preparation for degrading nitrile compounds. The XiNit1 protein serves as an active ingredient of the preparation. The nitrilase has great application prospect in the field of related chemical industry and the field of environment pollution control.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to nitrilase XiNit1 and its coding gene and application. Background technique

[0002] Nitrile compound is an important organic chemical raw material, but also a toxic substance with pungent smell. There are many different types of nitrile compounds in the sewage of synthetic rubber and petroleum refining process, and acrylonitrile is one of the important toxic components. Compared with the by-products of physical and chemical methods in the process of treating nitrile compounds, people prefer to use environmentally friendly biological methods to treat nitrile compounds in sewage. Compared with physical and chemical methods, biological methods have the characteristics of mild reaction conditions, stable operation and low cost. Since the 19th century, the use of biological methods to treat organic matter in wastewater has become the main method in all countries in the world...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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