Kit and method for detecting epithelial-mesenchymal mixed circulating tumor cells

Abstract

The invention discloses a kit for detecting epithelial-mesenchymic mixed circulating tumor cells, and a method for enriching and detecting epithelial-mesenchymal mixed and PD-L1 positive circulating tumor cells. The kit comprises a kit body and a kit body cover, a heat-insulating and waterproofing layer closely clings to the inner wall of the kit body, a cold box is arranged at the bottom in the kit body, a base box is arranged on the cold box, a storage grooves and a plurality of storage holes are arranged in the bottom box, spiral sample chambers and micro-fluidic chips are arranged in the storage grooves, a plurality of reagent bottles are arranged in the plurality of storage holes, and the plurality of reagent bottles form a reagent bottle group. The kit has the advantages of reasonable design combination, use and storage saving, and convenience in transportation, can be directly used for use, storage and transportation in the normal temperature environment, and ensures the effectiveness of the reagent consumable combination of the kit for detecting epithelial-mesenchymic mixed circulating tumor cells in the process for enriching, capturing and detecting epithelial, epithelial-mesenchymal mixed and mesenchyma circulating tumor cells.

Description

technical field [0001] The invention relates to the field of detection kits and epithelial-mesenchymal mixed circulating tumor cells, in particular to a detection kit for epithelial-mesenchymal mixed circulating tumor cells and enrichment detection of epithelial-mesenchymal mixed and PD-L1 positive circulating tumor cells method. Background technique [0002] Circulating Tumor Cells (CTCs) are tumor cells that are released from solid tumors or metastases into the peripheral blood circulation spontaneously or due to diagnostic and therapeutic procedures, and are a general term for various types of tumor cells that exist in peripheral blood. CTCs are mainly a heterogeneous population composed of epithelial CTCs, mixed epithelial-mesenchymal CTCs and mesenchymal CTCs subpopulations. [0003] Metastatic spread of tumors is a major cause of cancer progression and associated death. CTCs play an important role in the process of distant metastasis of tumors. If a large number of ...

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Problems solved by technology

[0004] The number of CTCs in peripheral blood is very rare, accounting for only 1 / 10 of peripheral blood leukocytes 6 ~1 / 10 7 , so the effective enrichment and detection technology bottleneck for different types of CTCs is higher and more difficult, which has always affected the practical application of CTCs

At present, the enrichment detection of CTCs mainly adopts the following two forms: ① relying on anti-EpCAM to enrich and capture CTCs and anti-cytokeratin (CK8, CK18, CK19, etc.) This method is only aimed at the enrichment and detection of epithelial CTCs, and cannot detect CTCs with mesenchymal phenotype and epithelial-mesenchymal mixed type; However, the enrichment specificity of this method is not high, CTCs with special biophysical characteristics will be missed, and there are still CTCs that cannot detect mixed epithelial-mesenchymal types

Therefore, the enrichment and detection of conventional CTCs are often limited to the enrichment and detection of a single group of CTCs, and cannot detect mixed epithelial-mesenchymal CTCs. Due to the heterogeneity and dynamic evolution of CTCs, single-type enrichment detection The method greatly limits the clinical application effect of CTCs

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