Rubber tree U6 gene promoter proHbU6.1 and clone and applications thereof

A technology of prohbu6.1-sk-5g, prohbu6.1-sgRNA-163cas9m, applied in the field of rubber tree RNA polymerase III type promoter, rubber tree U6 gene promoter proHbU6.1, can solve problems such as limited application, achieve high efficiency The effect of precise varieties and high transcriptional activity
CN110144355AActive Publication Date: 2019-08-20RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
Publication Date
2019-08-20

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Abstract

The invention belongs to the technical field of gene engineering, particularly relates to a rubber tree RNA polymerase III-type promoter, more specifically relates to a rubber tree U6 gene promoter proHbU6.1, and further discloses a cloning method and applications thereof. The invention clones and obtains the rubber tree RNA polymerase III-type promoter-rubber tree endogenous U6 promoter proHbU6.1in hevea brasiliensis for the first time, the promoter is rubber tree endogenous RNA polymerase III-type promoter, the promoter has high-efficiency transcription activity and can drive downstream sgRNA expression, the activity and the feasibility of the promoter for application in rubber tree CRISPR / Cas9 gene editing system are verified by transient transformation of rubber tree protoplasts, andCRISPR / Cas9 mediated rubber tree genome targeted editing is realized.
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Description

technical field

[0001] The invention belongs to the technical field of genetic engineering, specifically relates to a Hevea RNA polymerase type III promoter, more specifically relates to a Hevea U6 gene promoter proHbU6.1, and further discloses its cloning method and application. Background technique

[0002] Natural rubber has always been an important strategic material and reserve resource in my country. At present, the breeding of new varieties of rubber trees in my country is still based on traditional hybrid breeding. Due to the slow growth of rubber trees, conventional breeding has the problems of low efficiency and long cycle, which seriously hinders the progress of rubber tree breeding. With the development of biotechnology, the application of molecular breeding technology in rubber trees has accelerated the cultivation of new varieties of rubber trees. The introduction of exogenous genes through Agrobacterium or gene gun has become an important way for the genetic i...

Claims

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