In the present invention, by amplifying a segment of Rhodosporidium toruloides DNA sequence and performing biological function verification, the obtained genus Rhodosoporidium, Sporidiobolus, and Saccharomyces ( Sporobolomyces) and Rhodotorula (Rhodotorula), RNA polymerase type III promoters for non-coding RNA transcription, genetic engineering operations and strain improvement, the nucleotide sequence of which is SEQ ID NO:1. The present invention also relates to a method for constructing genetically engineered strains of the genus Rhodosporidium, Saccharomyces genus and Rhodotorula genus and the corresponding strains containing the DNA sequence expression cassette or recombinant vector, and using related elements.