Apocarya chromosomal karyotype analysis method

A technology of pecan shell and chromosome karyotype, which is applied in the direction of analyzing materials, material analysis by optical means, measuring devices, etc., can solve the problems of complicated operation, poor tableting effect, and poor mobility, etc.

Inactive Publication Date: 2019-08-23
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The tableting method is easy to operate and has good mobility, but the tableting effect is poor when dealing with some materials whose cell walls are hard and difficult to soften. The operation is cumbersome, the maneuverability is poor, and there are high requirements for film

Method used

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  • Apocarya chromosomal karyotype analysis method
  • Apocarya chromosomal karyotype analysis method
  • Apocarya chromosomal karyotype analysis method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] In this embodiment, the karyotype analysis of the chromosomes of 'Boni' thin-shell pecans is carried out, which specifically includes the following steps:

[0030] Step S1. material collection: in fine weather, at 8:00 in the morning, get the side buds of the 'Pony' shell pecan plant;

[0031] Step S2. Pretreatment: Treat the collected lateral buds with 0.01% colchicine at 4°C in a light-shielding environment for 4 hours;

[0032] Step S3. Fixing: put the pretreated lateral buds into Carnot's fixative (V 95％无水乙醇 :V 冰乙酸 =3:1) fixed for 24h;

[0033] Step S4. Dissociation: Pour off the fixative, and heat dissociation with 1mol / L hydrochloric acid in a 60°C water bath for 8 minutes;

[0034] Step S5. Staining: transfer the material to a glass slide, and stain with carbo fuchsin solution for 2 hours;

[0035] Step S6. Pressing: place the stained material and the glass slide on absorbent paper, cover the cover glass with tweezers, drive away the air bubbles, and disperse...

Embodiment 2

[0041] In this embodiment, the karyotype analysis of the 'Mahan' pecan chromosome is carried out, which specifically includes the following steps:

[0042] Step S1. taking materials: in fine weather, at 7:30 in the morning, get the side buds of the 'Mahan' thin shell pecan plant;

[0043] Step S2. Pretreatment: Treat the collected lateral buds with an equal-volume mixture of 0.01% colchicine + 2 mmol 8-hydroxyquinoline at 4°C in a light-shielding environment for 4 hours;

[0044] Step S3. Fixing: put the pretreated lateral buds into Carnot's fixative (V 95％无水乙醇 :V 冰乙酸 =3:1) fixed 26h;

[0045] Step S4. Dissociation: Pour off the fixative, and heat dissociation with 1mol / L hydrochloric acid in a 60°C water bath for 9 minutes;

[0046] Step S5. Staining: transfer the material to a glass slide, and stain with carbo fuchsin solution for 2 hours;

[0047] Step S6. Pressing: place the stained material and the glass slide on absorbent paper, cover the cover glass with tweezers, d...

Embodiment 3

[0053] In this embodiment, the karyotype analysis of the 'Jinhua' pecan chromosome is carried out, which specifically includes the following steps:

[0054] Step S1. taking materials: in fine weather, at 8:45 in the morning, get the top buds of 'Jinhua' thin-shell hickory;

[0055] Step S2. Pretreatment: Treat the collected terminal buds with an equal volume mixture of 0.01% colchicine + 2 mmol 8-hydroxyquinoline at 4°C in a light-shielding environment for 5 hours;

[0056] Step S3. fix: the terminal bud after pretreatment is put into Carnot's fixative (V 95％无水乙醇 :V 冰乙酸 =3:1) fixed for 24h;

[0057] Step S4. Dissociation: Pour off the fixative, and use 1mol / L hydrochloric acid in a 60°C water bath to dissociate for 7 minutes;

[0058] Step S5. Staining: transfer the material to a glass slide, and stain with carbo fuchsin solution for 2 hours;

[0059] Step S6. Pressing: place the stained material and the glass slide on absorbent paper, cover the cover glass with tweezers, ...

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Abstract

The invention relates to an apocarya chromosomal karyotype analysis method, comprising the following steps: in sunny whether, at 7:30-8:45 or 9:30 in the morning, taking lateral buds or terminal budsof a plant; treating the collected lateral buds or terminal buds for 4-6 hours with 0.01% colchicine, or treating for 4-6 hours with solution formed by mixing 0.01% colchicine and 2mmol 8-hydroxyquinoline in equal volume; putting the pretreated lateral buds or terminal buds into Carnoy fixative to be subjected to fixation for more than 24 hours; pouring away the fixative, and heating and dissociating for 7-9 minutes in a water bath at the temperature of 60 DEG C by using 1mol/L hydrochloric acid; transferring a material to a glass slide, and dyeing for 2 hours with carbol fuchsin solution; placing the material and the glass slide on absorbent paper, covering the glass slide, and driving away bubbles, so that cells are dispersed and flattened; and performing microscopic examination on a squash slide, and photographing. The apocarya chromosomal karyotype analysis method provided by the technical scheme of the invention performs karyotype analysis on a chromosome of apocarya by virtue ofa squashing method and provides a scientific basis for study on identification on a planting resource of the apocarya and genetic breeding of the apocarya.

Description

technical field [0001] The invention relates to the technical field of chromosome karyotype analysis, in particular to a method for analyzing the chromosome karyotype of pecan carya. Background technique [0002] Thin-shelled hickory (Carya illinoinensis), a plant of the genus Hickory in the family Juglandaceae, also known as American hickory, long hickory. Native to the United States and northern Mexico, it is one of the world's famous dried fruit tree species. Its nuts are large, with thin shells, high kernel yield, easy kernel extraction, and high yield; its nuts are delicious, fragrant, non-astringent, and nutritious, making them ideal health food. Thin-shelled pecan is also an important woody oil plant. The oil content of the seed kernel is as high as 70%, of which the unsaturated fatty acid content is as high as 97%. It is a high-quality edible oil that is resistant to storage. Thin-shelled hickory is also an excellent timber and greening tree species, integrating th...

Claims

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Application Information

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IPC IPC(8): G01N21/84
CPCG01N21/84G01N2021/8466
Inventor 谭鹏鹏彭方仁韩杰冯刚梁刚粱有旺朱凯凯
Owner NANJING FORESTRY UNIV
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