Preparation method of probiotics for pregnant and lying-in woman and children
A technology of probiotics and pregnant women, applied in the direction of bacteria, applications, and lactobacilli used in food preparation, can solve the problems of probiotics losing colonization, reducing the intestinal regulation ability of pregnant women and children, and achieve the regulation of intestinal health , Improve the stability and the number of live bacteria, and the effect of scientific proportioning
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Embodiment 1
[0031] S1 Weighing: Weigh 300kg of raspberry juice, 100kg of maltodextrin, 50kg of fructose, 10kg of inulin, 50kg of fructooligosaccharide, 3kg of vitamin C, 1kg of Lactobacillus acidophilus, 3kg of Lactobacillus reuteri and 3kg of Lactobacillus helveticus ,spare;
[0032] S2 compound inoculation: add the above-mentioned maltodextrin, mogrosin, inulin, fructooligosaccharides and vitamin C to the raspberry juice and mix evenly. After sterilization and cooling, inoculate Lactobacillus acidophilus, Lactobacillus reuteri and Swiss milk Bacillus, to obtain a mixed system;
[0033] S3 fermentation: Stir the above mixed system at a constant speed, and ferment at 25°C for 72 hours to obtain a fermentation broth;
[0034] S4 Freeze-drying: Mix the concentrated fermented liquid with 3% skim milk powder in a volume ratio of 1:3 to make a bacterial suspension; pre-freeze the bacterial suspension at -60°C for 5h, and then freeze Freeze at ℃ for 4 hours, freeze at 0℃ for 2 hours, dry at 1...
Embodiment 2
[0039] S1 Weighing: Weigh 800kg of raspberry juice, 120kg of maltodextrin, 30kg of mogrosin, 20kg of inulin, 30kg of fructooligosaccharide, 7kg of vitamin C, 3kg of Lactobacillus acidophilus, 1kg of Lactobacillus reuteri and 1kg of Lactobacillus helveticus ,spare;
[0040] S2 compound inoculation: add the above-mentioned maltodextrin, mogrosin, inulin, fructooligosaccharides and vitamin C to the raspberry juice and mix evenly. After sterilization and cooling, inoculate Lactobacillus acidophilus, Lactobacillus reuteri and Swiss milk Bacillus, to obtain a mixed system;
[0041] S3 fermentation: Stir the above mixed system at a constant speed, and ferment at 40°C for 36 hours to obtain a fermentation broth;
[0042] S4 Freeze-drying: Mix the concentrated fermented liquid with 3% skim milk powder in a volume ratio of 1:3 to make a bacterial suspension; pre-freeze the bacterial suspension at -60°C for 5h, and then freeze Freeze at ℃ for 4 hours, freeze at 0℃ for 2 hours, dry at 1...
Embodiment 3
[0047]S1 Weighing: Weigh 500kg of raspberry juice, 110kg of maltodextrin, 40kg of fructose, 15kg of inulin, 40kg of fructooligosaccharide, 5kg of vitamin C, 2kg of Lactobacillus acidophilus, 2kg of Lactobacillus reuteri and 2kg of Lactobacillus helveticus ,spare;
[0048] S2 compound inoculation: add the above-mentioned maltodextrin, mogrosin, inulin, fructooligosaccharides and vitamin C to the raspberry juice and mix evenly. After sterilization and cooling, inoculate Lactobacillus acidophilus, Lactobacillus reuteri and Swiss milk Bacillus, to obtain a mixed system;
[0049] S3 fermentation: Stir the above mixed system at a constant speed, and ferment at 37°C for 48 hours to obtain a fermentation broth;
[0050] S4 Freeze-drying: Mix the concentrated fermented liquid with 3% skim milk powder in a volume ratio of 1:3 to make a bacterial suspension; pre-freeze the bacterial suspension at -60°C for 5h, and then freeze Freeze at ℃ for 4 hours, freeze at 0℃ for 2 hours, dry at 10...
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