Medicine for treating diabetic peripheral neuropathy, preparation method and use thereof
A technology for peripheral neuropathy and diabetes, applied in nervous system diseases, drug combinations, pharmaceutical formulas, etc., can solve the problems of increasing the risk of side effects, increasing the cost of medication, etc., to achieve changes in microcirculation, wide application, and increase conduction velocity Effect
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Embodiment 1
[0022] Embodiment 1 is used for the preparation of the medicine of diabetic peripheral neuropathy
[0023] Weigh 10 kg of cassia medicinal material, soak for 24 hours, ethanol concentration 80% (volume fraction), flow rate 3ml / (min·kg), and ethanol consumption 8 times the percolation method to obtain cassia to obtain ethanol extract. The cassia seeds are soaked in 2-8 times the volume of cold water first, and extracted by any method of warm soaking extraction, decoction extraction and reflux extraction to obtain a water extract. All the obtained water extracts and ethanol extracts were combined to obtain a drug for the treatment of DPN.
Embodiment 2
[0024] Embodiment 2 to the treatment of diabetic peripheral neuropathy
[0025] 1 material
[0026] 1.1 Animals 6-week-old male SPF-grade obese type 2 diabetic C57BL / 6ob / ob mice and C57BL / 6 wild-type mice were provided by the Experimental Animal Center of Zhejiang University of Traditional Chinese Medicine. The breeding room is kept well ventilated, the room temperature is controlled at (21±1)°C, the humidity is 63%, the noise is ≦55dB, free to eat and drink, and the time of light and dark alternates every 12h.
[0027] 1.2 Reagents and instruments Han's analgesic instrument (Nanjing Jisheng Medical Technology Co., Ltd., HANS-100 type). The drug used to treat diabetic peripheral neuropathy is extracted from the School of Medicine of Zhengzhou Institute of Technology, and is prepared with distilled water to a concentration of 20mg·mL before gavage -1 suspension; α-lipoic acid capsules, specification: 0.3g per capsule, batch number: 170313, produced by Penglai Nuokang Pharmace...
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