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Extraction method of four-component decoction

A technology of Sijunzi decoction and extraction method, which is applied in the direction of medical formula, medical preparations containing active ingredients, plant raw materials, etc., can solve the problems of scientifically evaluating the pros and cons of the preparation process, the quality of the preparation, and the control of the extraction process, and achieve alcohol The sinking process is stable and feasible, with good repeatability and high paste yield

Pending Publication Date: 2020-07-10
CHONGQING ACAD OF CHINESE MATERIA MEDICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is difficult to control the extraction process of each medicinal material with a few index components, and it is even more difficult to scientifically evaluate the pros and cons of the preparation process and the quality of the preparation, and then obtain the optimal extraction process related to the functions and indications of the prescription

Method used

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  • Extraction method of four-component decoction
  • Extraction method of four-component decoction
  • Extraction method of four-component decoction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 water extraction process

[0030] Determination of Yield of Water Extraction Dry Cream

[0031] Take by weighing 9 parts of medicinal materials in the prescription amount, each containing 9 g of ginseng, 9 g of Atractylodes macrocephala, 9 g of poria cocos, and 6 g of licorice, decoct according to the conditions listed in the orthogonal design table, filter the medicinal liquid, and combine the filtrate (the volume is denoted as V 1 ). Accurately measure an appropriate amount of extract (volume is denoted as V 2 ) into an evaporating dish with a constant mass, evaporated to dryness in a water bath, baked at 105°C for 3 hours at normal pressure, taken out, cooled in a desiccator for 30 minutes, weighed accurately, dried at 100-105°C for 1 hour, cooled, and weighed until Until the difference between two consecutive weighings is no more than 5mg, the dry paste of the test product is obtained (the weight is denoted as M 1 ), according to the formula to calcu...

Embodiment 2

[0051] Alcohol Precipitation and Refining Process Optimization

[0052] Determination of Yield of Alcohol Precipitation Dry Paste

[0053] Get the preferred water extraction concentrate, then carry out alcohol precipitation according to the orthogonal test arrangement, suction filtration, and the filtrate is concentrated under reduced pressure (50 ° C) to an appropriate amount (volume is recorded as V 1 / ), precision measures an appropriate amount of alcohol precipitation solution (volume is recorded as V 2 / ) to an evaporating dish with constant mass, vacuum (0.08-0.10MPa, 105°C) to dry to constant mass, place in a desiccator to cool for 30min, weigh accurately, then dry at 100-105°C for 1h, cool, and weigh until Until the difference between two consecutive weighings is no more than 5mg, the dry paste of the test product is obtained (the weight is denoted as M 1 / ), according to the formula to calculate the ointment rate.

[0054]

[0055] Alcohol Precipitation Or...

Embodiment 3

[0073] Determination of Ginsenoside Rg1, Ginsenoside Rb1, Ginsenoside Re, Liquiritin and Glycyrrhizic Acid

[0074] Preparation of the test solution

[0075] Accurately weigh the dry paste powder after water extraction or water extraction and alcohol precipitation (through a 65-mesh sieve), put it in a 50mL conical flask with a stopper, accurately add 25mL of methanol, weigh it, and ultrasonically treat it for 20min (power 200W, frequency 40kHz) , let it cool, weigh again, make up the lost mass with methanol, shake well, and filter. Precisely measure 5mL of the continued filtrate, put it in a 25mL measuring bottle, add methanol to the mark, shake well, and you get it.

[0076] Preparation of reference solution

[0077] Precisely weigh an appropriate amount of ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, glycyrrhizin and glycyrrhizic acid reference substance, dissolve in methanol to obtain a mass concentration of 0.638, 0.6, 0.906, 0.597, and 0.603 mg·mL -1 mixed refere...

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Abstract

The invention provides an extraction method of a four-component decoction. Through control of a series of conditions such as soaking time, water addition amount, extraction time and extraction times,the process is stable and feasible, the repeatability is good, and the extract yield is relatively high while the contents of active pharmaceutical ingredients such as ginsenoside Rg1, Re, Rb1, glycyrrhizic acid and liquiritin are effectively guaranteed. Compared with a model group, the four-component decoction water extract group has the advantages that the sialidase activity and the serum gastrin content of rats are remarkably increased, and the small intestine xylose absorption function of rats with spleen deficiency can be improved; compared with the four-component decoction water extractgroup, the four-component decoction water extract alcohol precipitate group has remarkable difference in D-xylose excretion rate, but the sialidase activity and the serum gastrin content have no remarkable difference. The invention further provides a detection method of the four-component decoction. The method is good in precision, repeatability and accuracy, relatively high in system adaptabilityand specificity and high in practicability.

Description

technical field [0001] The invention belongs to the technical field of extraction of traditional Chinese medicines, and in particular relates to an extraction method of Sijunzi Decoction. Background technique [0002] Sijunzi Decoction originated from the "Taiping Huimin Heji Bureau Prescription" in the Song Dynasty. It is composed of four herbs: the monarch drug ginseng, the minister drug Atractylodes macrocephala, the adjuvant drug Poria cocos and the envoy drug roasted licorice. The four medicines complement each other and have the effect of nourishing qi and invigorating the spleen. It is mainly used for the treatment of spleen deficiency, weak stomach, weak speech, weakness of limbs, pale tongue, weak and thready pulse, sharp loss of appetite, full abdomen and borborygmus, accompanied by cold syndrome. Sijunzi Decoction is widely used clinically, and has been developed into ointments, mixtures, pills, tablets, capsules, granules and other prescription preparations. Amon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/484A61P1/00A61P1/14G01N30/74
CPCA61K36/076A61K36/258A61K36/284A61K36/484A61K2236/331A61K2236/39A61K2236/53A61P1/00A61P1/14G01N30/74A61K2300/00
Inventor 尚芳红阳勇宋旭红王计瑞谭均谭春斌
Owner CHONGQING ACAD OF CHINESE MATERIA MEDICA
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