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Single cell protein from thermophilic fungi

A thermophilic fungus, protein technology, applied in the field of microbiology and fermentation, can solve problems such as inappropriate

Pending Publication Date: 2019-09-03
蛋白啤酒厂有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since Aspergillus fumigatus is a human pathogen, it is not suitable for producing SCP for food or feed

Method used

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  • Single cell protein from thermophilic fungi
  • Single cell protein from thermophilic fungi
  • Single cell protein from thermophilic fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Example 1. Production of protein-enriched animal feed from sugar beet pulp

[0071] Thaw 1 ml of frozen Rasamsonia composticola CBS 141695 mycelium (-80°C, glycerol stock solution) and inoculate into 35 ml of yeast extract / glucose medium (each 20 g / L), the medium was sterilized at 121° C. for 20 minutes, and incubated at 45° C. at 220 rpm with an eccentric distance of 25 mm for 48 hours.

[0072] 10 ml of this 48 hour preculture was transferred to the sugar beet pulp medium as follows:

[0073] Prepare 250ml of medium in a 2L baffled Erlenmeyer flask containing sufficient mineral solution of K, P, S, Ca, Mg, Zn, Fe, Mn, Cu (for good fungal growth (US20020039758)), 1.75 gr / L NaNO 3 , 1.75gr / L di-ammonium sulfate, 0.1gr / L yeast extract and 20gr / L Fibrex 500 (commercial beet pulp Nordic Sugar milled to a fine paste). The medium was supplemented with 10 drops of soybean oil to prevent foaming and sterilized at 121°C for 20 minutes.

[0074] After fermentation, the ferme...

Embodiment 2

[0077] Example 2. Production of SCP in a fed-batch process.

[0078] Beet pulp was liquefied at 45°C to 27% dry matter using 0.02g VisCO Reductase AC100 (Weiss Biotech) per gram of syrup from the silage process. The beet pulp was diluted with water to a dry matter concentration of 8% and shaken in a shaker incubator at 150 rpm with a 25 mm eccentric distance. At the beginning of liquefaction, the pH of the silage was 3.8, and after 3 hours, the liquefied beet pulp was added to the hydrolysis reactor, diluted with water to 5.2% dry matter, and stirred at 400 rpm in a 15 L stirred tank reactor, The temperature was set at 60° C. for another 6 hours and the pH was controlled to pH 4 using 8% ammonia as titrant. The hydrolyzate was then harvested and frozen until used as feed to the fermentation.

[0079]A 1 ml frozen vial (glycerol stock solution at -80°C) of strain Rasamsonia composticola CBS141695 was thawed and added to 35 mL of medium containing yeast extract 20 g / L and gluc...

Embodiment 3

[0082] Example 3. Production of fish from dried SCP

[0083] 525 g of the 33% dry matter biomass filter cake obtained in Example 2 were mixed with wheat gluten 12.4 gr, commercially available fish feed (27 gr) and 3.5 g of calcium carbonate, extruded through a 1 mm extruder and fluidized in Dry in a bed drier at 30°C. 145 g of dry SCP were obtained with about 30.8% protein on dry matter basis. 80% of all protein in this diet comes from SCP. The dried filter cake was ground and sieved on a 0.5mm sieve.

[0084] After feeding a total of 15.4 grams of fresh weight of 7 tilapias per day at 27-28° C. for 30 days, 58.7 grams of fish were obtained, whereas a total of 109.5 grams of feed was added, demonstrating 0.54 grams of fresh fish per gram of feed. The fish are very lively and healthy, love the food and have normal droppings.

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Abstract

The present invention relates to a process for producing single cell protein, wherein a thermophilic fungus is grown a fermentable carbon-rich feedstock at a high temperature and at an acidic pH. Thisallows for a cost effective fermentation process that can to be run under non-sterile conditions and without additional cooling requirements. The process can be used to convert byproducts or waste from agriculture or food production, or organic fractions of municipal solid waste into valuable single cell protein that can be applied as dietary source of protein or protein supplement in human foodor animal feed.

Description

[0001] Field of Invention [0002] The invention relates to the fields of microbiology and fermentation technology. In particular, the present invention relates to the production of single-cell proteins for food products and animal feed through the fermentation of carbon-rich and energy-rich feedstocks by thermophilic fungi. Background technique [0003] Increasing global population and wealth has led to a rapidly increasing demand for protein-rich foods such as meat, dairy products, insects and fish. Thus, in countries such as Brazil, the increase in soybean production has led to the loss of tropical rainforests in order to be able to produce soybeans on a large scale, which are exported to other parts of the world. Therefore, there is a need for more locally produced protein-rich animal feed. One approach to produce protein-rich animal feed is the production of "single-cell protein" (SPC) by fermentation (Suman et al., 2015, Int J.Curr.Microbiol.Appl.Sci., Vol 4., No 9. ,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23J1/00A23L31/00C12R1/845C12R1/645
CPCA23J1/008C12N1/14A23L31/00C12R2001/845C12N1/145C12R2001/645A23K20/147A23K10/16C12P21/00A23V2250/546A23V2002/00A23L33/195A23V2250/54
Inventor W·T·A·M·德拉特J·S·加莱戈穆里洛
Owner 蛋白啤酒厂有限公司
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