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Preparation method of total saponins of panax stipuleanatus and application thereof

A technology of Panax notoginseng saponins and Panax notoginseng saponins, which is applied in the field of preparation of Panax notoginseng saponins, can solve the problems of limited efficacy and liver protection effect of Panax notoginseng in Pingbian, etc., achieves less toxic and side effects, and can be prepared The effect of simple method

Inactive Publication Date: 2019-09-06
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are few drugs clinically used to treat acute and chronic liver injuries, and their efficacy is limited. Therefore, it is urgent to develop new and safe drugs against various acute and chronic liver diseases. to report

Method used

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  • Preparation method of total saponins of panax stipuleanatus and application thereof
  • Preparation method of total saponins of panax stipuleanatus and application thereof
  • Preparation method of total saponins of panax stipuleanatus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Pingbian notoginsenoside R 1 and R 2 The extraction and separation method is as follows:

[0023] Pingbian Panax notoginseng root and rhizome powder (1kg), extracted with methanol 3 times (10L each time), concentrated under reduced pressure to obtain methanol extract (250.6g), added 2L of water to dissolve the methanol extract, and then extracted and degreased by petroleum ether , saturated n-butanol extraction to obtain n-butanol extract (30g), the n-butanol extract was separated by silica gel column chromatography, followed by dichloromethane:methanol (volume ratio of 95:5, 90:10, 80:20 , 60:40, 50:50) gradient elution to obtain the active component of Pingbian Sanqi (11.7g), the effective component of Pingbian Notoginseng (11.7g) was separated by ODS column chromatography (the solvent system is methanol by volume : water = 55:45, 75:25, 80:20, 90:10, 100:0) as mobile phase elution to obtain Pingbian notoginseng saponin R 1 (1.5g), Pingbian notoginseng saponin R 1...

Embodiment 2

[0026] The method of preparing Pingbian Panax notoginseng saponins by reflux extraction method is as follows:

[0027] Take Pingbian Panax notoginseng main root and rhizome powder (200g), add 6L ethanol aqueous solution, the ethanol aqueous solution is obtained by mixing ethanol and water according to the volume ratio of 70:100, heat and reflux at 60°C for 3 hours, filter and remove the residue The extraction was repeated three times, and the combined filtrates were concentrated and dried under reduced pressure to obtain the extract of Panax notoginseng extract (95.3 g).

[0028] Pingbian notoginseng extract (95.3 g) was separated by macroporous resin column chromatography (solvent system by volume ratio is ethanol: water = 0:100, 20:80, 40:60, 60:40, 70:30, 80:20, 90:10, 100:0 elution), collect the ethanol eluate with ethanol volume fraction of 70%~90% in the ethanol / water solvent system, and the eluate is conventionally concentrated and freeze-dried to obtain Pingbian 3 Sev...

Embodiment 3

[0031] The method for preparing Pingbian Panax notoginseng saponins by ultrasonic extraction is as follows:

[0032] Take Pingbian Panax notoginseng root and rhizome powder (200g), add 2L ethanol water solution, ethanol water solution is obtained by mixing ethanol and water according to the volume ratio of 70:100, at 50°C, ultrasonic extraction for 30min, power 250W, frequency 50KHz, repeat the extraction 3 times, filter, combine the filtrates, concentrate and dry under reduced pressure to obtain the extract of Panax notoginseng extract (82.8 g).

[0033] Pingbian notoginseng extract extract (82.8 g) was separated by macroporous resin column chromatography (solvent system by volume ratio is ethanol: water = 0:100, 20:80, 40:60, 60:40, 70:30, 80:20, 90:10, 100:0 elution), collect the ethanol eluate with ethanol volume fraction of 70%~90% in the ethanol / water solvent system, and the eluate is conventionally concentrated and freeze-dried to obtain Pingbian 3 Seven total saponins...

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Abstract

The invention discloses a preparation method of total saponin of panax stipuleanatus and an application thereof. The preparation method comprises the steps of extracting the panax stipuleanatus powderinto a solvent, and after filtering, extracting the residue for 2 to 3 times, combining the filtrates, performing vacuum concentration to obtain an extract, separating the extract by using macroporous resin, collecting an eluate by using an ethanol / water solution as a solvent system, and performing condensation and freeze drying to obtain the total saponin of panax stipuleanatus. The preparationmethod of the invention has simple operation and expands the application range of the panax stipuleanatus, and the extract can be applied in the medicine for treating and preventing various liver diseases.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a preparation method and application of Pingbian Panax notoginseng total saponins. Background technique [0002] Pingbian Panax notoginseng ( Panax stipuleanatus H. T. Tsai et K. M. Feng) is a plant of the genus Ginseng in the Araliaceae family. It is also known as wild notoginseng, xiangthorn, and soil notoginseng. At present, scholars at home and abroad are focusing on its chemical composition (Yang Chongren, et al. Yunnan Botanical Research, 1985, (01):103-108.), planting methods (invention patents CN201610036240.6, CN201610700439. 4), rapid identification method (invention patent CN201611218427.4), conservation method (invention patent CN201610702302.2), drying method (invention patent CN201510563209.3), anti-inflammatory activity (invention patent CN201711210126.1), anticancer activity (Liang C, et al.J Gins Res, 2013, 37(1): 74-79.) and blood lipid and bl...

Claims

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Application Information

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IPC IPC(8): A61K36/258A61P1/16A61K31/704
CPCA61K36/258A61P1/16A61K2236/331A61K2236/333A61K2236/55A61K2236/39A61K31/704
Inventor 曲媛杨鑫龙刘迪秋杨野朱敏崔秀明王承潇
Owner KUNMING UNIV OF SCI & TECH
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